Endogenous Opiates and Behavior: 2016

Bodnar, Richard J.

doi:10.1016/j.peptides.2018.01.011

Cited by 54 publications

(32 citation statements)

References 1,076 publications

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“…The Oprm1 transcript was present at GV stage and granulosa cells, whereas at MI and MII stages no signal was detected. This fact has been described previously for other transcripts (Agirregoitia et al, 2012;López-Cardona et al, 2017, 2016 since mRNA is selective degraded during oocyte maturation and transcription becomes silent from the resumption of meiosis until two-cell stage in mice (Gosden & Lee, 2010;Picton, Briggs, & Gosden, 1998). Mu opioid receptor transcript has also been detected in oocytes and granulosa cells from other species, such as bovine, equine, human, or porcine (Agirregoitia et al, 2012;Dang-Nguyen et al, 2013;Dell'Aquila et al, 2008Lunger et al, 2016).…”

Section: Effect Of Opioid Agonist Morphine During Oocyte Maturationsupporting

confidence: 73%

“…Relative results to control group (0 nM) are shown and results are the means ± SEM of five independent experiments. The different combinations of letters indicate significant differences between groups; p < .05 in all cases cannabinoids, very related to opioids due to their similar action mechanism, was able to accelerate the AKT activation both in granulosa cells and oocytes during maturation in bovine and mice (López-Cardona et al, 2017, 2016.…”

Section: Effect Of Opioid Antagonist Naloxone During Oocyte Maturatmentioning

confidence: 96%

“…When those receptors are activated, among other effects, the adenylyl cyclase is inhibited, thus reducing cyclic AMP and blocking PKA (Madishetti et al, 2014;Mehlmann, 2005). Moreover, opioids are also able to activate both PI3K/Akt and MAPK signaling pathways (Bodnar, 2013).…”

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confidence: 99%

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Implication of mu opioid receptor in the in vitro maturation of oocytes and its effects on subsequent fertilization and embryo development in mice

Olabarrieta

Totorikaguena

Agirregoitia

et al. 2019

Molecular Reproduction Devel

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Oocyte maturation is the process by which immature oocytes acquire all the necessary characteristics for successful fertilization. The endogenous opioid peptides have been suggested to have a role modulating this process. However, little is known about its implication and the effect of exposing oocyte maturation to opioids on the subsequent fertilization and embryo development. Hence, in the present work, we focused on elucidating the function of the mu opioid receptor (OPRM1) in the modulation of the oocyte maturation. We analyzed the expression and localization of OPRM1 in mice oocytes and granulosa cells by reverse‐transcription polymerase chain reaction (RT‐PCR) and immunocytochemistry. To observe the activity of the OPRM1, immature oocytes were incubated with morphine agonist and/or naloxone antagonist and we evaluated the PI3K/Akt and MAPK pathways, as well as the effect on the subsequent fertilization and embryo development. OPRM1 was present in mice oocytes and granulosa cells, changing its expression pattern depending on the maturation stage. Moreover, morphine, modulating PI3K/Akt and MAPK pathways, helped oocytes to reach blastocyst stage, which was reverted by naloxone. These results propose the OPRM1 as a possible therapeutic target for in vitro maturation culture medium, as it could improve the blastocyst rates obtained in the actual reproduction assisted techniques.

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Section: Effect Of Opioid Agonist Morphine During Oocyte Maturationsupporting

confidence: 73%

Section: Effect Of Opioid Antagonist Naloxone During Oocyte Maturatmentioning

confidence: 96%

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Implication of mu opioid receptor in the in vitro maturation of oocytes and its effects on subsequent fertilization and embryo development in mice

Olabarrieta

Totorikaguena

Agirregoitia

et al. 2019

Molecular Reproduction Devel

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“…From the human brain, 70‐fold higher contents of these peptides were purified, and it was reported that differences in concentration between the bovine and human peptides were attributed to a variety of factors . After the isolation of endomorphin peptides, a variety of biochemical, immunohistochemical, pharmacological, and behavioral research was performed by using many different approaches . Endomorphin‐1 and ‐2 activate both G αi1 ‐ and G αoA ‐type G α proteins through the μ‐opioid receptor .…”

Section: Initial Discovery Of Endomorphins In Bovine Brainsmentioning

confidence: 99%

“…[5] After the isolation of endomorphin peptides, av ariety of biochemical, immunohistochemical, pharmacological, and behavioral research was performed by using many differenta pproaches. [12][13][14] Endomorphin-1a nd -2 activate both G ai1 -a nd G aoA -type G a proteins through the m-opioid receptor. [15] The distributions of endomorphin-1 and -2 have been analyzed in detail by using anti-endomorphin antibodies.…”

Section: Initial Discovery Of Endomorphins In Bovine Brainsmentioning

confidence: 99%

Biosynthetic Short Neuropeptides: A Rational Theory Based on Experimental Results for the Missing Pain‐Relief Opioid Endomorphin Precursor Gene

2019

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Endomorphins are neuropeptides that bind strongly to μ‐opioid receptors and are considered to play important roles in pain modulation and other biological functions. Two endomorphins have been identified, to date, endomorphine‐1 and ‐2; both are tetrapeptides and differ by only a single amino acid in the third position. Both peptides were isolated from bovine brains; however, their precursor genes have not been identified. In this study, a nucleotide sequence corresponding to the endomorphin‐1 peptide in an expressed sequence tag database has been found and a preproendomorphin‐like precursor peptide from human brain complementary DNA (cDNA) has been cloned. The cDNA consists of nucleotide sequences of two already annotated predicted genes, and the putative peptide differs by one amino acid from the isolated endomorphin peptides. It is proposed herein that there is the possibility of unknown short proteins or peptide precursors being missed by automated gene prediction programs based on similarities of known protein sequences. A novel concept of how to produce endomorphins from a similar peptide is described. The oxidatively modified base might provide a clue for understanding discrepancies between nucleotide sequences on the genome and those on cDNAs.

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