Endogenous levels of insect juvenile hormones in larval, pupal and adult stages of the tomato moth, Lacanobia oleracea

Edwards, John; Corbitt, T. S.; McArdle, H.F.; Short, J. E.; Weaver, Rob

doi:10.1016/0022-1910(95)00031-o

Cited by 54 publications

(46 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shu et al (1998) showed 5-15-fold increase in JH titer in mated female H. virescens compared with virgins. Similarly, in another noctuid, Lacanobia olaracea, mated females exhibited approximately 8-to 10-fold increase in total JH titer compared with virgin females (Edwards et al, 1995). In vitro, 3-day-old virgin female CA synthesized and released JH II as the predominant homologue, followed by JH III and much less JH I (Fig.…”

Section: Discussionmentioning

confidence: 92%

Mating inHeliothis virescens: Transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone

Park

Shu

Ramaswamy

et al. 1998

Arch. Insect Biochem. Physiol.

View full text Add to dashboard Cite

Studies were undertaken to determine whether adult males of Heliothis virescens transfer juvenile hormone (JH) to females during copulation, and an in vitro radiochemical assay was used to determine whether mating causes an allatotropic effect, i.e., stimulation of JH biosynthesis by corpora allata (CA). In vitro, CA from 3‐day‐old mated females synthesized and released approximately 2.5 times total JH as that of CA from comparably aged virgin females. Of the homologues, JH II exhibited significant increase in mated females; JH I also increased but not significantly. JH III remained similar to that of virgin females. This is the first demonstration of an allatotropic effect of mating in moths. In contrast to the female, CA of virgin males did not produce any JH, but accessory sex glands (ASG) in 3‐day‐old males synthesized small amounts of JH. Immediately after adult emergence, male ASG contained approximately 1.5 ng JH I and II, which increased by 12 h after emergence and remained at this high level up to 54 h after emergence. JH III was barely detected in ASG. JH in ASG of mated male immediately after uncoupling was depleted almost completely, and 24 h later recovered to levels comparable to that of 54‐h‐old virgin male. Virgin female bursa copulatrix did not contain any JH, but mated female bursa, immediately after uncoupling, had JH at levels comparable to that observed in virgin male ASG. By 6 h after uncoupling, JH levels decreased dramatically in mated female bursa. These data suggest the transfer of JH to females by the male. Arch. Insect Biochem. Physiol. 38:100–107, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 92%

Mating inHeliothis virescens: Transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone

Park

Shu

Ramaswamy

et al. 1998

Arch. Insect Biochem. Physiol.

View full text Add to dashboard Cite

show abstract

“…Venkatesh et al (1988) suggested that mating in Trichoplusia ni resulted in an increase in JH titer owing to significant suppression of JH-esterase levels in mated females. Edwards et al (1995) also reported that JH I and II levels increased in mated Lacanobia oleracea female adults. They found JH I titers in mated females were approximately two-, seven-, ten-, and fourteenfold higher than those in virgin females on the second, third, fourth, and fifth days after emergence.…”

Section: Discussionmentioning

confidence: 91%

Vitellogenin and egg production in the moth,Heliothis virescens

Zeng

Shu

Park

et al. 1997

Arch. Insect Biochem. Physiol.

View full text Add to dashboard Cite

The characteristics of vitellogenin (Vg) and the relationship between Vg production and egg production in the tobacco budworm, Heliothis virescens, were studied. The relationship between Vg production and juvenile hormone (JH) and the impact of mating on Vg and egg production were also investigated. Vg appears in the hemolymph of H. virescens about 6 h after moth eclosion. Vg may be separated into two apoproteins (ApoVg‐I and ApoVg‐II) by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). The molecular weights were calculated to be 156,065 ± 800 for ApoVg‐I and 39,887 ± 323 for ApoVg‐II. SDS‐PAGE analysis revealed that the female hemolymph Vg polypeptides appear to be identical to those from eggs but are absent in male hemolymph. Vg concentration was significantly higher in mated females than in virgin females of the same age at 48 h after emergence. Rates of egg production increased as Vg production increased; rates of egg production in mated females were significantly higher than those of virgin females at 48, 72, 96, and 120 h postemergence. Vg production is dependent on JH, because hemolymph from decapitated females lacked Vg while that of decapitated females treated with synthetic JH had Vg at levels comparable to similarly aged, normal H. virescens females. Hemolymph JH titers in mated females were significantly higher compared with those in virgin females at all sampling periods. The high JH level in mated females may explain the high Vg and egg production in mated H. virescens. Arch. Insect Biochem. Physiol. 34:287–300, 1997. © 1997 Wiley‐Liss, Inc.

show abstract

“…The second effect has not been reported upon previously and may be caused by the factor responsible of the increased levels of juvenile hormone that are observed in hosts parasitized at the start of the final larval stadium [4]. The malformed pupae observed following the injection of certain fractions are highly reminiscent of larvae that have been exposed to abnormally high levels of JH during their final instar, a time when JH is virtually absent in larval Lepidoptera [12,21]. It is, therefore, likely that this second biological activity results from a JH-modulating factor that targets either JH biosynthesis or its metabolism by juvenile hormone esterase, both effects that have been recorded from L. oleracea parasitized by E. pennicornis [4] and a number of other parasitoid-host associations [6,22].…”

Section: Discussionmentioning

confidence: 97%

The Biological Effects of Venom Derived from the Ectoparasitic Wasp Eulophus pennicornis (Nees) (Hymenoptera: Eulophidae): Evidence for Dual Endocrine Regulation~!2008-08-08~!2009-05-30~!2010-02-11~!

Bell¹,

Powell²,

Price³

et al. 2010

TOENTOJ

View full text Add to dashboard Cite

Abstract:The venom of the ectoparasitoid Eulophus pennicornis is known to induce developmental arrest in parasitized hosts through the prevention of moulting. However, little work has explored how the venom affects different host stages, whether the observed effects vary with different doses, and whether the venom has activity in non-host species.Injections of venom doses of 0.5 g protein prevented the development of >90% of both fifth and sixth instar Lacanobia oleracea larvae. In fifth instars, developmental arrest at doses of 1.0 g occurred prior to ecdysis to the sixth stadium, whilst the 0.5 and 0.25 g treatments allowed 36% and 77% of injected larvae, respectively, to moult although most subsequently failed to pupate. Although naturally parasitized hosts always showed reduced growth, lower doses of venom often prevented pupation without affecting this parameter, particularly in sixth instar larvae where only the highest dose (2.0 g) reduced the mean maximum weights achieved. Two factitious lepidopteran hosts, Manduca sexta and Spodoptera littoralis, were also affected by injection of the venom at w/w doses equivalent to those used for L. oleracea although larvallarval moulting was only prevented in the former.Fractionation of venom using gel-filtration FPLC indicated the presence of at least two factors that markedly affected host development. One series of contiguous fractions, on injection, prevented larval-larval ecdysis in a similar way to whole venom. A second series of fractions had no effect on larval-larval ecdysis but resulted in the formation of deformed pupae, an effect that closely resembled the application of a juvenile hormone (JH) analogue, which may indicate the presence of a factor within the venom that modulates host levels of this hormone.

show abstract

Endogenous levels of insect juvenile hormones in larval, pupal and adult stages of the tomato moth, Lacanobia oleracea

Cited by 54 publications

References 51 publications

Mating inHeliothis virescens: Transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone

Mating inHeliothis virescens: Transfer of juvenile hormone during copulation by male to female and stimulation of biosynthesis of endogenous juvenile hormone

Vitellogenin and egg production in the moth,Heliothis virescens

The Biological Effects of Venom Derived from the Ectoparasitic Wasp Eulophus pennicornis (Nees) (Hymenoptera: Eulophidae): Evidence for Dual Endocrine Regulation~!2008-08-08~!2009-05-30~!2010-02-11~!

Contact Info

Product

Resources

About