Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells

Cen, Sheng-Dan; Yu, Wen-Bin; Ren, Manman; Chen, Lijiao; Sun, Chaofan; Ye, Zhi-Li; Deng, Hui; Hu, Rongdang

doi:10.1016/j.archoralbio.2016.03.009

Cited by 14 publications

(11 citation statements)

References 23 publications

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“…Bae and coworkers determined through in vivo experiments that BMP-2 containing thiolated chitosan scaffolds induced more ectopic bone formation in mouse osteoblast cells than the BMP-2 containing collagen gels [52]. We presume that the influence of these thiol groups is related to the release of hydrogen sulfide, an intracellular gaseous signaling molecule involved in osteogenic differentiation [53, 54]. To clarify this hypothesis, we performed a parallel investigation of spontaneous and induced osteogenic differentiation of the PDLCs on thiolated PASP based hydrogels.…”

Section: Resultsmentioning

confidence: 99%

Free thiol groups on poly(aspartamide) based hydrogels facilitate tooth-derived progenitor cell proliferation and differentiation

et al. 2019

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Cell-based tissue reconstruction is an important field of regenerative medicine. Stem and progenitor cells derived from tooth-associated tissues have strong regeneration potential. However, their in vivo application requires the development of novel scaffolds that will provide a suitable three-dimensional (3D) environment allowing not only the survival of the cells but eliciting their proliferation and differentiation. Our aim was to study the viability and differentiation capacity of periodontal ligament cells (PDLCs) cultured on recently developed biocompatible and biodegradable poly(aspartamide) (PASP)-based hydrogels. Viability and behavior of PDLCs were investigated on PASP-based hydrogels possessing different chemical, physical and mechanical properties. Based on our previous results, the effect of thiol group density in the polymer matrix on cell viability, morphology and differentiation ability is in the focus of our article. The chemical composition and 3D structures of the hydrogels were determined by FT Raman spectroscopy and Scanning Electron Microscopy. Morphology of the cells was examined by phase contrast microscopy. To visualize cell growth and migration patterns through the hydrogels, two-photon microscopy were utilized. Cell viability analysis was performed according to a standardized protocol using WST-1 reagent. PDLCs were able to attach and grow on PASP-based hydrogels. An increase in gel stiffness enhanced adhesion and proliferation of the cells. However, the highest population of viable cells was observed on the PASP gels containing free thiol groups. The presence of thiol groups does not only enhance viability but also facilitates the osteogenic direction of the differentiating cells. These cell-gel structures seem to be highly promising for cell-based tissue reconstruction purposes in the field of regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Free thiol groups on poly(aspartamide) based hydrogels facilitate tooth-derived progenitor cell proliferation and differentiation

et al. 2019

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“…Impaired H 2 S synthesis due to a deficiency of CBS is linked to impaired osteogenesis and bone loss in mice [ 28 ]. The WNT pathway [ 27 , 58 ], BMP pathway [ 59 ], and p38-MAPK and ERK signaling pathways [ 57 , 60 ], as well as S-sulfhydration of Runx-2 [ 54 ] and Ca 2+ TRP channels [ 28 ], have been implicated in H 2 S-dependent induction of osteogenesis. H 2 S has been found to reverse nicotine- and lipopolysaccharide-induced osteoclastic differentiation [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Association of Hydrogen Sulfide with Femoral Bone Mineral Density in Osteoporosis Patients: A Preliminary Study

Hao

Gao

et al. 2021

Med Sci Monit

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Background Accumulated evidence has suggested that hydrogen sulfide (H 2 S) has a role in bone formation and bone tissue regeneration. However, it is unknown whether the H 2 S content is associated with bone mineral density (BMD) in patients with osteopenia/osteoporosis. Material/Methods In the present study, we aimed to explore the changes of serum H 2 S in osteopenia and osteoporosis patients. We analyzed femur expression of cystathionine β synthase (CBS), cystathionine γ lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST), which are key enzymes for generating H 2 S. Results Sixteen (16%) patients had osteopenia, 9 (9%) had osteoporosis, and 75 (75%) had normal BMD. In comparison with patients with normal BMD (controls), the serum levels of H 2 S were unexpectedly increased in patients with osteopenia and osteoporosis. This increase was much higher in patients with osteoporosis than in those with osteopenia. Serum H 2 S levels were negatively correlated with femoral BMD, but not lumbar BMD. Interestingly, the expression of CBS and CSE were downregulated in femur tissues in patients with osteoporosis, whereas the expression of 3-MST remained unchanged. Serum phosphorus levels, alkaline phosphatase, hemoglobin, and triglycerides were found to be closely associated with CBS and CSE scores in femur tissues. Conclusions Serum H 2 S levels and femur CBS and CSE expression may be involved in osteoporosis pathogenesis.

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“…Notably, H 2 S-deficiency due to CBS knock-down was linked to impaired osteogenesis and bone loss in mice [15]. Several pathways are implicated in H 2 S-dependent induction of osteogenesis, summarized in Figure 2: the WNT pathway [14,88]; BMP pathway [77]; and p38-MAPK and ERK signaling pathways [84,89]. Moreover, S-sulfhydration of cysteine residues in critical proteins involved in osteogenesis, such as Runx-2 [81] and Ca 2+ TRP channels [15], has been shown to be a mechanism of H 2 S-dependent induction of osteogenesis.…”

Section: Bone Tissue Repair: H2s As a Suitable Biological Cue For mentioning

confidence: 99%

“…Schematic representation of the main mechanisms of action of H 2 S in mesenchymal stromal cells, osteoblasts, and osteoclasts. H 2 S modulates osteogenic differentiation in osteoprogenitor cells by inducing S-sulfhydration in TRP channels leading to increased Ca 2+ influx in MSC [15]; at the transcriptional level, H 2 S donors induce the expression of osteogenic signaling through stimulation of multiple signaling pathway [14,77,88]. In the context of inflammation, H 2 S triggers an antioxidant signaling in OC which involves the increased nuclear translocations of NRF-2 and results in the inhibition of OC differentiation [39,87].…”

Section: Figurementioning

confidence: 99%

Hydrogen Sulfide in Bone Tissue Regeneration and Repair: State of the Art and New Perspectives

Gambari

Grigolo

Grassi

2019

IJMS

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The importance of hydrogen sulfide (H2S) in the regulation of multiple physiological functions has been clearly recognized in the over 20 years since it was first identified as a novel gasotransmitter. In bone tissue H2S exerts a cytoprotective effect and promotes bone formation. Just recently, the scientific community has begun to appreciate its role as a therapeutic agent in bone pathologies. Pharmacological administration of H2S achieved encouraging results in preclinical studies in the treatment of systemic bone diseases, such as osteoporosis; however, a local delivery of H2S at sites of bone damage may provide additional opportunities of treatment. Here, we highlight how H2S stimulates multiple signaling pathways involved in various stages of the processes of bone repair. Moreover, we discuss how material science and chemistry have recently developed biomaterials and H2S-donors with improved features, laying the ground for the development of H2S-releasing devices for bone regenerative medicine. This review is intended to give a state-of-the-art description of the pro-regenerative properties of H2S, with a focus on bone tissue, and to discuss the potential of H2S-releasing scaffolds as a support for bone repair.

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Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells

Cited by 14 publications

References 23 publications

Free thiol groups on poly(aspartamide) based hydrogels facilitate tooth-derived progenitor cell proliferation and differentiation

Free thiol groups on poly(aspartamide) based hydrogels facilitate tooth-derived progenitor cell proliferation and differentiation

Association of Hydrogen Sulfide with Femoral Bone Mineral Density in Osteoporosis Patients: A Preliminary Study

Hydrogen Sulfide in Bone Tissue Regeneration and Repair: State of the Art and New Perspectives

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