Endogenous hydrogen sulfide as a mediator of vas deferens smooth muscle relaxation

Li, Jingxin; Li, Yan; Du, Yahui; Mou, Kun; Sun, Hui; Zang, Yuanwei; Liu, Chuanyong

doi:10.1016/j.fertnstert.2010.11.001

Cited by 19 publications

(18 citation statements)

References 8 publications

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“…KCl, adrenergic and cholinergic agonists would usually be chosen to precontract smooth muscle in not only LUT but also cardiovascular vessels, gastrointestinal tract, and corpus cavernosum tissues. 2,5,6,7,23,24 Taken together, we proposed that the effect of H 2 S on human and rat bladder smooth muscle tone might play various roles, depending on different pretreatments. 20,21 In our study, exogenous and endogenous H 2 S relaxed smooth muscle that was pre-contracted with acetylcholine chloride.…”

Section: Discussionmentioning

confidence: 82%

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Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Gai

Wahafu²,

Guo³

et al. 2013

Asian J Androl

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We investigated the expression of hydrogen sulphide (H 2 S) in human and rat lower urinary tract (including bladder, prostate and urethra) tissues, and we sought to determine whether H 2 S induces relaxation of human and Sprague-Dawley (SD) rat bladder strips. Human normal lower urinary tract tissue was obtained for the evaluation of endogenous H 2 S productivity using a sulphide-sensitive electrode and for the analysis of the expression levels of all three synthases of endogenous H 2 S, cystathionine b-synthase (CBS), cystathionine c lyase (CSE) and 3-mercaptopyruvate sulphur transferase (MPST, as known as 3-MST) by Western blot assay. CBS, CSE and MPST were located in human sample slides by immunohistochemistry. Human and male adult SD rat bladder strips were tested for H 2 S function with a transducer and recorded. All experiments were repeated six times. The endogenous H 2 S productivity and the H 2 S synthases had various distributions in the human and rat lower urinary tract tissues and were located in both epithelial and stromal sections. L-cysteine (L-Cys, a substrate of CBS, CSE and MPST) elicited relaxation in a dose-dependent manner on human bladder strips pre-contracted by acetylcholine chloride. This effect could be diminished by the ATP-sensitive potassium ion (K ATP ) channel blocker glibenclamide (GLB), the CSE inhibitor DL-propargylglycine (PPG) and the CBS inhibitor hydroxylamine (HA). H 2 S and its three synthases were present in the human and rat lower urinary tract tissues and relaxed human and rat bladder strips, which implied that endogenous H 2 S might play a role in physiological function and pathological disorders of the lower urinary tract symptoms (LUTS) or overactive bladder (OAB).

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Section: Discussionmentioning

confidence: 82%

“…[4][5][6][7] Bucci et al 8 even noted that H 2 S was involved in testosterone vascular effects, which made H 2 S more attractive for urological researchers. Our previous study had also revealed the expression and characterisation of H 2 S in human prostatic tissue and cell lines.…”

Section: Introductionmentioning

confidence: 99%

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Gai

Wahafu²,

Guo³

et al. 2013

Asian J Androl

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“…Both CBS and CSE are functionally expressed in the vas deferens of rat, mice, and human. The endogenous H 2 S causes a smooth muscle relaxation of vas deferens (Li et al 2011). The same group has also demonstrated that NaHS-induced effect is mediated by BK Ca channel.…”

Section: Hydrogen Sulfide and Vas Deferensmentioning

confidence: 89%

Hydrogen Sulfide and Urogenital Tract

Bianca

Cirino

Sorrentino

2015

Chemistry, Biochemistry and Pharmacology of Hydrogen Sulfide

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In this chapter the role played by H2S in the physiopathology of urogenital tract revising animal and human data available in the current relevant literature is discussed. H2S pathway has been demonstrated to be involved in the mechanism underlying penile erection in human and experimental animal. Both cystathionine-β synthase (CBS) and cystathionine-γ lyase (CSE) are expressed in the human corpus cavernosum and exogenous H2S relaxes isolated human corpus cavernosum strips in an endothelium-independent manner. Hydrogen sulfide pathway also accounts for the direct vasodilatory effect operated by testosterone on isolated vessels. Convincing evidence suggests that H2S can influence the cGMP pathway by inhibiting the phosphodiesterase 5 (PDE-5) activity. All these findings taken together suggest an important role for the H2S pathway in human corpus cavernosum homeostasis. However, H2S effect is not confined to human corpus cavernosum but also plays an important role in human bladder. Human bladder expresses mainly CBS and generates in vitro detectable amount of H2S. In addition the bladder relaxant effect of the PDE-5 inhibitor sildenafil involves H2S as mediator. In conclusion the H2S pathway is not only involved in penile erection but also plays a role in bladder homeostasis. In addition the finding that it involved in the mechanism of action of PDE-5 inhibitors strongly suggests that modulation of this pathway can represent a therapeutic target for the treatment of erectile dysfunction and bladder diseases.

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“…In the present study, we demonstrated that both CSE and CBS were expressed primarily in the human oviduct epithelium. H 2 S has been shown to cause relaxation of arterial blood vessels 2,21,22 , small bronchial rings [23][24][25] , human corpus cavernosum 7 , rat vas deferens 11 , and gastrointestinal and urogenital smooth muscle 26 . The human fallopian tube is capable of synthesizing H 2 S. In our in vitro experiments, the production of H 2 S also relaxed the spontaneous contraction of the human oviduct, which suggests that an endogenous H 2 S relaxation system functionally exists in the fallopian tube.…”

Section: Discussionmentioning

confidence: 99%

“…H 2 S produced by 3MST regulates mitochondrial reactive oxygen species (ROS) production 3 . Intriguingly, the expression of H 2 S-generating enzymes and the endogenous production of H 2 S have recently been identified in male and female reproductive systems in several mammalian species [6][7][8][9][10][11] . In the male reproductive duct, endogenously produced H 2 S mediates erectile function 7,[12][13][14] , spermatogonial proliferation 6 and vas deferens smooth-muscle relaxation.…”

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confidence: 99%

Dysregulation of hydrogen sulphide metabolism impairs oviductal transport of embryos

Ning

Zhu

et al. 2014

Nat Commun

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Embryo retention in the fallopian tube is thought to lead to ectopic pregnancy, which is a significant cause of morbidity. Hydrogen sulphide (H 2 S) is a gaseotransmitter produced mainly by cystathionine-g-lyase and cystathionine-b-synthase. Here we show that cystathionine-g-lyase and cystathionine -b-synthase are ubiquitously distributed in human fallopian tube epithelium and that H 2 S signalling relaxes the spontaneous contraction of the human oviduct. Furthermore, an aberration in H 2 S signalling, either silenced or enhanced activity induced by pharmacologic or genetic methods, causes embryo retention and developmental delay in the mouse oviduct, which is partly reversed by administration of either GYY4137, a slow-releasing H 2 S donor, or NaHS. Our findings reveal a new regulatory mechanism for oviductal embryo transport.

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Endogenous hydrogen sulfide as a mediator of vas deferens smooth muscle relaxation

Cited by 19 publications

References 8 publications

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Hydrogen Sulfide and Urogenital Tract

Dysregulation of hydrogen sulphide metabolism impairs oviductal transport of embryos

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