Endogenous glycosides in critically ill patients

Berendes, Elmar; Cullen, Paul; Aken, Hugo Van; Zidek, Walter; Erren, Michael; Hübschen, Michael; Weber, Thomas; Wirtz, Stefan; Tepel, Martín; Walter, Michael

doi:10.1097/01.ccm.0000059721.57219.c3

Cited by 40 publications

(26 citation statements)

References 33 publications

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“…(1) The heterogeneity of the ouabain antibody used in different laboratories [7,23,[37][38][39]45,53,[56][57][58][59][60][61][62][63][64][65][66][67][68][69]73,74,76,78,98,99], some of which have marked differences in affinities and specificities. Nicholls et al [8] note the lack of a commercial endogenous ouabain assay.…”

Section: Detectability Of Endogenous Ouabain In Biological Fluids or mentioning

confidence: 99%

“…Other polar cardiotonic steroids of unknown structure are present in bovine tissue [25] and human plasma (Hamlyn et al, unpublished observation). (3) The immunoassay format, that is, radioimmunoassay [37,45,53,[56][57][58]62,[65][66][67][68][69]76,79,100] vs. ELISA [7,23,46,60,61,64,75,77,98], EIA [59,99] or DELFIA [63] used. Different formats have certain advantages but also tend to report method-specific values and differ in susceptibility to interferants.…”

Section: Detectability Of Endogenous Ouabain In Biological Fluids or mentioning

confidence: 99%

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Endogenous ouabain in cardiovascular function and disease

Manunta

Ferrandi²,

Bianchi

et al. 2009

Journal of Hypertension

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An endogenous ouabain has been isolated and conclusively identified from several mammalian tissues, including human plasma, by a number of independent laboratories. Substantial evidence from independent laboratories in several continents is consistent with an adrenal source for most if not all of the circulating endogenous ouabain. Accumulating evidence suggests that circulating levels of endogenous ouabain in humans are modulated by dietary salt and chronic volume status. Endogenous ouabain is linked significantly with vascular function in hypertension and likely impacts the pathogenesis of heart and renal failure. Moreover, the molecular mechanism of endogenous ouabain-linked hypertension involves the sodium pump/sodium-calcium exchange duet. The outstanding analytical issues include the elucidation of distal events in the biosynthetic pathway for endogenous ouabain and identification of molecular mechanisms that regulate its secretion and clearance.

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Section: Detectability Of Endogenous Ouabain In Biological Fluids or mentioning

confidence: 99%

Section: Detectability Of Endogenous Ouabain In Biological Fluids or mentioning

confidence: 99%

Endogenous ouabain in cardiovascular function and disease

Manunta

Ferrandi²,

Bianchi

et al. 2009

Journal of Hypertension

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“…By itself, the observation of a J-shaped relation of endogenous ouabain with salt balance is interesting but hardly noteworthy. However, in virtually all clinical studies in which endogenous ouabain is chronically elevated, outcome is universally poor [15,56,57 ■■ ]. This raises the possibility that high endogenous ouabain per se either is a biomarker and/or a functional mediator of adverse cardiovascular outcomes.…”

Section: Salt Blood Pressure Endogenous Ouabain and The J Curvementioning

confidence: 99%

Salt sensitivity, endogenous ouabain and hypertension

Hamlyn¹,

Blaustein²

2012

Current Opinion in Nephrology and Hypertension

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Purpose of review Endogenous cardiotonic steroids (CTS) exert long-term effects on salt and blood pressure homeostasis. Here we discuss recent observations on mechanisms of salt sensitivity that involve endogenous ouabain and novel pathways in the brain and discuss their possible relationship to arterial and renal function in hypertension. Recent findings Chronic elevation of brain sodium promotes sustained hypertension mediated by central endogenous ouabain and the Na+ pump α-2 catalytic subunit. The intermediary pressor mechanism in the brain involves aldosterone biosynthesis, activation of mineralocorticoid receptors and increased epithelial sodium channel activity. In the periphery, elevated plasma CTS raise contractility and blood pressure by augmentation of sympathetic nerve responses, increasing arterial Ca2+ signaling and blunting nitric oxide production in the renal medulla and collecting ducts. Summary Endogenous ouabain in the brain appears to play a critical role in salt sensitivity and hypertension. In the periphery, the J-shaped relationship of plasma endogenous ouabain in response to short-term changes in salt balance in humans raises the possibility that endogenous ouabain contributes to the increased risk of adverse cardiovascular events associated with both low and high salt intakes.

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“…We have suggested that digoxin should not be given to patients in whom the ambient concentrations of endogenous CGs are increased (15 ). This highlights a need for the development of analytical methods that use clinically relevant volumes of blood for the routine determination of endogenous CGs so that the appropriate information concerning optimal therapy can be obtained (12 ).…”

mentioning

confidence: 99%

Biosynthesis of Endogenous Cardiac Glycosides by Mammalian Adrenocortical Cells: Three Steps Forward

Hamlyn

2004

Clinical Chemistry

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Endogenous glycosides in critically ill patients

Cited by 40 publications

References 33 publications

Endogenous ouabain in cardiovascular function and disease

Endogenous ouabain in cardiovascular function and disease

Salt sensitivity, endogenous ouabain and hypertension

Biosynthesis of Endogenous Cardiac Glycosides by Mammalian Adrenocortical Cells: Three Steps Forward

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