Endogenous Fluorescence Lifetime Imaging (FLIM) Endoscopy For Early Detection Of Oral Cancer And Dysplasia

Jo, Javier A.; Cheng, Shannon; Cuenca-Martinez, Rodrigo; Duran-Sierra, Elvis; Malik, Bilal; Ahmed, Beena; Maitland, Kristen C.; Cheng, Yi-Shing Lisa; Wright, John M.; Reese, Terry

doi:10.1109/embc.2018.8513027

Cited by 27 publications

(30 citation statements)

References 18 publications

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“…With the development of ms-FLIM, it was applied to differentiate low-risk oral lesions, high-risk oral lesions, and oral carcinoma with high sensitivity and specificity in the hamster cheek pouch model [ 65 ]. The feasibility of ms-FLIM for early oral cancerous tissue detection was further testified by clinical trials [ 66 , 67 ]. Besides, ms-FLIM dermoscopy system has been successfully constructed for discriminating basal cell carcinoma (BCC) from other dermatoses [ 68 ].…”

Section: Flim Combined With Nad(p)h For Cancer Metabolism Monitoringmentioning

confidence: 99%

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

et al. 2021

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Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence. Because fluorescence lifetime is sensitive to microenvironments and molecule alterations, FLIM is promising for the detection of pathological conditions. Current cancer-related FLIM applications can be divided into three main categories: (i) FLIM with autofluorescence molecules in or out of a cell, especially with reduced form of nicotinamide adenine dinucleotide, and flavin adenine dinucleotide for cellular metabolism research; (ii) FLIM with Förster resonance energy transfer for monitoring protein interactions; and (iii) FLIM with fluorophore-dyed probes for specific aberration detection. Advancements in nanomaterial production and efficient calculation systems, as well as novel cancer biomarker discoveries, have promoted FLIM optimization, offering more opportunities for medical research and applications to cancer diagnosis and treatment monitoring. This review summarizes cutting-edge researches from 2015 to 2020 on cancer-related FLIM applications and the potential of FLIM for future cancer diagnosis methods and anti-cancer therapy development. We also highlight current challenges and provide perspectives for further investigation.

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Section: Flim Combined With Nad(p)h For Cancer Metabolism Monitoringmentioning

confidence: 99%

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

et al. 2021

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“…Moreover, macroscopic deeper imaging approaches are suitable for non-invasive imaging, allowing for imaging across the intact skin, which maintains the undisrupted native physiology and environment of organs and tissues, albeit at the cost of resolution. The flexibility of FLI has allowed its use in endoscopy for multiple in vivo applications via a fiber-optic bundle (Duran-Sierra et al, 2020;Hage et al, 2019;Jo et al, 2018;Won et al, 2016). FMT is a non-invasive and non-ionizing optical imaging modality that retrieves the biomarkers of interest in 3D volumes.…”

Section: Contrast Enhancement For High-performance 3d Imaging and Super-resolution Microscopymentioning

confidence: 99%

Luminescence lifetime imaging of three-dimensional biological objects

Dmitriev

Intes

Barroso

2021

Journal of Cell Science

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A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs and organisms in the context of their complex spatiotemporal biological and molecular features. In particular, the modalities of luminescence lifetime imaging, comprising fluorescence lifetime imaging (FLI) and phosphorescence lifetime imaging microscopy (PLIM), in synergy with Förster resonance energy transfer (FRET) assays, provide a wealth of information. On the application side, the luminescence lifetime of endogenous molecules inside cells and tissues, overexpressed fluorescent protein fusion biosensor constructs or probes delivered externally provide molecular insights at multiple scales into protein–protein interaction networks, cellular metabolism, dynamics of molecular oxygen and hypoxia, physiologically important ions, and other physical and physiological parameters. Luminescence lifetime imaging offers a unique window into the physiological and structural environment of cells and tissues, enabling a new level of functional and molecular analysis in addition to providing 3D spatially resolved and longitudinal measurements that can range from microscopic to macroscopic scale. We provide an overview of luminescence lifetime imaging and summarize key biological applications from cells and tissues to organisms.

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“…Included studies (Table 1) were published between 2006 and 2020, and they were focused on the Autofluorescence technique (n:14) [28][29][30][31][32][33][34][35][36][37][38][39][40][41], HRME (n:5) [42][43][44][45][46], Optical spectroscopy (n:7) [10, [47][48][49][50][51][52], NBI (n:2) [53,54], and Vital Stain Colorants (n:6) [55][56][57][58][59][60]. In total, 34 studies were performed in adults (18 years of age or older).…”

Section: Study Characteristicsmentioning

confidence: 99%

In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

Mazur

Ndokaj

Venugopal

et al. 2021

IJERPH

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Objectives: Oral potentially malignant disorders (OPMDs) are lesions that may undergo malignant transformation to oral cancer. The early diagnosis and surveillance of OPMDs reduce the morbidity and mortality of patients. Diagnostic techniques based on medical images analysis have been developed to diagnose clinical conditions. This systematic review and meta-analysis aimed to evaluate the efficacy of imaging-based techniques compared to the gold standard of histopathology to assess their ability to correctly identify the presence of OPMDs. Design: Literature searches of free text and MeSH terms were performed using MedLine (PubMed), Scopus, Google Scholar, and the Cochrane Library (from 2000 to 30 June 2020). The keywords used in the search strategy were: (“oral screening devices” or “autofluorescence” or “chemiluminescence” or “optical imaging” or “imaging technique”) and (“oral dysplasia” or “oral malignant lesions” or “oral precancerosis”). Results: The search strategy identified 1282 potential articles. After analyzing the results and applying the eligibility criteria, the remaining 43 papers were included in the qualitative synthesis, and 34 of these were included in the meta-analysis. Conclusions: None of the analyzed techniques based on assessing oral images can replace the biopsy. Further studies are needed to explore the role of techniques-based imaging analysis to identify an early noninvasive screening method.

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Endogenous Fluorescence Lifetime Imaging (FLIM) Endoscopy For Early Detection Of Oral Cancer And Dysplasia

Cited by 27 publications

References 18 publications

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

Luminescence lifetime imaging of three-dimensional biological objects

In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

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