Endogenous Fluorescence Dissimilarity Assessment of Four Potential Biomarkers of Early Liver Fibrosis by Preservation Media Effect

The technological advancements made in optics and semiconductors (e.g., cameras and laser diodes) working with infrared have brought interest in optical bioimaging back to the forefront of research investigating in vivo medical imaging techniques. The definition of the near-infrared transparency windows has turned optical imaging into more than just a method for topical imaging applications. Moreover, this has focused attention back to tissue fluorescence, emissions by tissues and organs that occur when excited by external illumination sources. Most endogenous fluorophores emit in the blue to green range of the electromagnetic spectrum and the resulting tissue fluorescence can be employed in studies from cells to tissue metabolism or avoided by shifting to the red if seen as unwanted autofluorescence. With the more recent move to infrared, it was discovered that autofluorescence is not limited to the visible but also strongly affects in vivo imaging in the infrared. In this Tutorial, we give an overview on tissue fluorescence and tissue interactions with excitation light as well as their effect on in vivo imaging. Furthermore, potential sources of tissue fluorescence in the near-infrared are identified and we describe approaches for successful biomedical imaging in the biological windows, taking into consideration infrared autofluorescence and summarizing techniques for avoiding it in in vivo imaging experiments.

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The role of tissue fluorescence in in vivo optical bioimaging

Lifante

Shen

Ximendes

et al. 2020

Journal of Applied Physics

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Method for measuring the extinction coefficient of fluorescing media within the emission band

Reyes¹,

Garcı́a-Valenzuela²,

Gutierrez-Herrera³

2023

Appl. Opt.

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We propose and test a method for determining a fluorescent medium’s absorption or extinction index while it is fluorescing. The method uses an optical arrangement that records changes in fluorescence intensity at a fixed viewing angle as a function of the angle of incidence of an excitation light beam. We tested the proposed method on polymeric films doped with Rhodamine 6G (R6G). We found a strong anisotropy in the fluorescence emission and, thus, limited the method to TE-polarized excitation light. The method proposed is model dependent, and we provide a simplified model for its use in this work. We report the extinction index of the fluorescing samples at a selected wavelength within the emission band of the fluorophore R6G. We found that the extinction index at the emission wavelengths in our samples is appreciably larger than the extinction index at the excitation wavelength, which is the opposite of what one might expect from measuring the absorption spectrum of the medium with a spectrofluorometer. The proposed method could be applied to fluorescent media with additional absorption other than by the fluorophore.

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Endogenous Fluorescence Dissimilarity Assessment of Four Potential Biomarkers of Early Liver Fibrosis by Preservation Media Effect

Cited by 2 publications

References 40 publications

The role of tissue fluorescence in in vivo optical bioimaging

The role of tissue fluorescence in in vivo optical bioimaging

Method for measuring the extinction coefficient of fluorescing media within the emission band

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