Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation

Perrin, Yolande; Doumas, Patrick; Lardets, Ludovic; Carrons, Marc-Philippe

doi:10.1007/bf02318978

Cited by 33 publications

(14 citation statements)

References 19 publications

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“…Part of the earliness could be explained by heterosis, but also it may have been due to the propagation method used for these hybrids. Indeed, somatic embryogenesis causes some secondary effects that have yet to be clarified and properly measured but which seem to play a role in tissue rejuvenation (Hackett Wesley and Murray John 1993;Perrin et al 1997). When working with the same genotype, we found that, for nursery coffee plants of the same height, plants derived from somatic embryos were significantly more vigorous than those derived from seeds (Menéndez-Yuffá et al 2010).…”

Section: Discussionmentioning

confidence: 79%

Performance of Coffea arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars

et al. 2011

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Coffea arabica F1 hybrids derived from crosses between wild Sudan-Ethiopian and American cultivars and propagated by somatic embryogenesis have been obtained in Central America. These new hybrids considerably enhanced the genetic diversity of coffee in the region. We conducted 15 trials to assess whether using hybrids represents substantial genetic progress in terms of productivity in agroforestry and full-sun cropping systems. The new germplasm was grown in the same conditions as the best American cultivar (homozygous pure lines). The results showed that yields of hybrids were earlier and superior to those of American cultivars. The hybrids were also more stable than the American cultivars in all environments.In the agroforestry system, the mean yield of hybrids was 58% higher than that of the American cultivars, while the mean yield of hybrids in the full-sun system was 34% higher. Coffee-based agroforestry systems (AS) are considered effective in protecting the environment in the volcanic cordilleras of Central America. We found that introducing hybrids in coffee-based AS can considerably increase productivity. This finding could be a convincing argument to encourage coffee growers who have adopted the full-sun cropping system to return to agroforestry cropping systems. Finally, the conditions for large-scale dissemination of those new hybrids-which represent a major innovation for C. arabica cropping-was analysed.

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Section: Discussionmentioning

confidence: 79%

Performance of Coffea arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars

et al. 2011

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“…This finding is in keeping with those of Werner et al (2001), who concluded that the growth of leaves in cytokinindeficient tobacco plants not only required cytokinins, but also the fine adjustment of natural cytokinin levels. Similarly, Perrin et al (1997) have also reported that the recovery of capacity for axillary shoot organogenesis by in vitro rubber tree shoots is related to an increase in endogenous zeatin levels.…”

Section: Discussionmentioning

confidence: 93%

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Viéitez

Corredoira

Ballester

et al. 2009

Plant Cell Tiss Organ Cult

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North American oak species, with their characteristic strong episodic seasonal shoot growth, are highly problematic for clonal micropropagation, resulting in the inability to achieve a stabilized shoot multiplication stage. The potential for initiating and proliferating shoot cultures derived from Quercus alba, Q. bicolor and Q. rubra explants was investigated, and a micropropagation method for these species was developed. Branch segments from 6 to 7-year-old trees were forced-flushed and the forced shoots were used as source of explants for culture initiation. A consistent shoot multiplication stage was achieved, in 13 of the 15 genotypes established in vitro, although marked differences occurred in explants from different genotypes/species. The control of efficient shoot multiplication involved the culture of decapitated shoots in a stressful horizontal position on cytokinin-containing medium with a sequence of transfers within a 6-week subculture cycle, which was beneficial to overcoming the episodic character of shoot growth. During each subculture cycle, the horizontally placed explants were cultured on media containing 0.2 mg l -1 benzyladenine (BA) for 2 weeks with two successive transfers (2 weeks each) to fresh medium with 0.1 mg l -1 BA, giving a 6-week subculture cycle. The general appearance and vigor of Q. alba and Q. bicolor shoot cultures were improved by the inclusion of both 0.1 mg l -1 BA and 0.5 mg l -1 zeatin in the medium used for the second transfer within the 6-week subculture cycle. Addition of AgNO 3 (3 mg l -1 ) to the shoot proliferation medium of Q. rubra had a significant positive effect on shoot development pattern by reducing deleterious symptoms, including shoot tip necrosis and early senescence of leaves. The three species showed acceptable in vitro rooting rates by culturing microcuttings in medium containing 25 mg l -1 indolebutyric acid for 48 h with subsequent transfer to auxin-free medium supplemented with 0.4% activated charcoal. Although an initial 5-day dark period generally improved the rooting response, it was detrimental to the quality of regenerated plantlets. However, activated charcoal stimulated not only the rooting frequencies, but it also enhanced plant quality, as evidenced by root, shoot and leaf growth.

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“…An estimate of the level of rejuvenation achieved after certain treatment should not be based only on the in vitro micropropagation criteria but also on studies of biochemical (Perrin et al 1997), and genetic and/or epigenetic markers of juvenility. In a study on biochemical markers to differentiate mature (Sainza-C) and juvenile (Sainza-BS) shoot lines, Vidal et al (2003) detected higher amounts of endogenous indoleacetic acid (IAA) in C shoots than in BS shoots during the rooting process.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, data demonstrating rejuvenation in plants derived from germinated somatic embryos of mature tree origin are scarce. SE has been induced in mature specimens of Hevea brasiliensis and rejuvenation was reflected in the re-acquisition of the micropropagation capacity (Carron et al 1995;Perrin et al 1997). The ability of somatic plants and SE-derived clones to undergo organogenesis in vitro has become of great interest in Theobroma cacao (Traore et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Improved organogenic capacity of shoot cultures from mature pedunculate oak trees through somatic embryogenesis as rejuvenation technique

Martínez

Vidal

Ballester

et al. 2011

Trees

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Theoretically, complete rejuvenation of mature trees should occur through somatic embryogenesis, however, this has not been extensively studied. The main objective of the present study was to increase the efficiency of in vitro clonal propagation for mature Quercus robur (100-300 years old), by induction of somatic embryogenesis as rejuvenation step prior to establishment of shoot culture through micropropagation of somatic embryo-derived plantlets. Shoot culture lines of ''mature'' origin were established from epicormic shoots of two centenarian oak genotypes (Sainza and CR-0) and maintained by axillary shoot proliferation. Embryogenic lines were also initiated from epicormic leaf explants of the same genotypes and maintained by secondary somatic embryogenesis. Although the frequency of somatic embryo conversion into plantlets was low in pedunculate oak, shoot culture lines could be established and maintained by axillary branching from several germinated somatic embryos. For each genotype and shoot culture line of the two origins (mature tree and somatic plantlets), shoot multiplication rate and elongation as well as rooting ability parameters were compared. Compared with ''mature-origin'' shoot cultures and after more than one year propagation in vitro, shoot lines established from somatic plantlets produced a significantly higher proportion of elongated, rootable shoots (from 26.0-31.6 to 36.8-40.5%) with increased rooting ability (from 3.3-45.6% to 23.2-89.8%). In the case of 300-year-old Sainza genotype such a high organogenic capacity was similar to shoot cultures initiated from basal sprouts. Basal sprouts are considered as ''mature'' material that retains juvenile characteristics compared with epicormic shoots forced from crown branches. Somatic embryogenesis only slightly improved plant regeneration of shoot cultures from basal sprouts, thus validating their use as ''juvenile control''. The present results provide evidence that some rejuvenation occurred during the process of somatic embryogenesis and resulted in improved shoot growth and rooting of somatic embryo-derived culture compared with ''mature'' shoot culture. The results reported in this study might be useful in embryogenic systems with low plant conversion rates. The proposed experimental model might also be useful in finding molecular markers of plant ontogeny.

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Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation

Cited by 33 publications

References 19 publications

Performance of Coffea arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars

Performance of Coffea arabica F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars

In vitro regeneration of the important North American oak species Quercus alba, Quercus bicolor and Quercus rubra

Improved organogenic capacity of shoot cultures from mature pedunculate oak trees through somatic embryogenesis as rejuvenation technique

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