Endogenous cytokinin levels in embryogenic and non-embryogenic calli of Medicago arborea L.

Pintos, Beatríz; Martín, Juan José Molina; Centeno, Marı́a Luz; Villalobos, Nieves; Guerra, Hilario; Martı́n, Luisa

doi:10.1016/s0168-9452(02)00244-3

Cited by 23 publications

(16 citation statements)

References 30 publications

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“…Third part of acquisition phase and an early stage of SE expression were also correlated with a greater concentration of cytokinins Z-type compared with iPA. This result resembles previous data indicating that the ratio of these two types of cytokinins is a good index of both the explant and callus embryogenic capacity (Centeno et al 1997;Pintos et al 2002). Among Z-type cytokinins, t-ZR appears to be particularly associated with frequent cell divisions leading to the production of linear stage somatic embryos in C. delgadii.…”

Section: Summary Of Physiological Events During Early Se Of C Delgadiisupporting

confidence: 89%

“…However, most of them are limited to the comparisons of the non-embryogenic and embryogenic callus (Jiménez and Bangerth 2000;Pintos et al 2002;Jiménez et al 2005), the non-embryogenic explants and those with somatic embryos developed on the explant surface (Pérez-Jimé-nez et al 2013), or the genotypes/explants with different embryogenic capability (Wenck et al 1988;Centeno et al 1997;Limanton-Grevet et al 2000;Jiménez and Bangerth 2001a). Only a few reports on the progressive hormonal changes that occur during SE are available, but they differ in the type of explant, method (plant growth regulators or other stress factors) and manner of somatic embryo induction (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Because the ratio of endogenous hormones controls a mode of morphogenetic response, the ratio of IAA or ABA to cytokinins (Żur et al 2015), or the ratio of ABA to IAA (Centeno et al 1997), or of various types of cytokinins (e.g. zeatin (Z)-type and isopentenyl (iP)-type; Grieb et al 1997;Pintos et al 2002) tend to be good indicators of the embryogenic competence of explants.…”

Section: Introductionmentioning

confidence: 99%

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The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

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of explant excision, a dramatic reduction in concentration of all phytohormones studied was observed, but hormonal balance and sugar content remained almost unchanged. During the 14-day-long culture, the ABA/CKs and ABA/ IAA ratios remained constant, whereas the greatest differences were detected for the IAA/CKs and Z-type/iPA cytokinin ratios. Excluding day 6 of culture, cytokinins were found to be the predominant phytohormones over IAA. An almost 12-fold increase in soluble sucrose concentration at day 6 of culture might be the switch to the SE expression phase. Frequent cell divisions leading to somatic embryo formation are clearly associated with increase in trans-zeatin riboside content. Keywords

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Section: Summary Of Physiological Events During Early Se Of C Delgadiisupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

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“…This alters the hormonal balance in the tissue of plants and prompts various physiological events, including the differentiation of the roots. The level of endogenous of 2ip was higher in zeatin in the callus of Medicago arborea and had inversion tendency with the time (Pintos et al, 2002). This was due to the 2ip obtained during the synthesis of zeatin (Haberer and Kieber, 2002;Mok and Mok, 2001).…”

Section: Resultsmentioning

confidence: 92%

Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA) to detect the cytokinins in Gerbera jamesonii plantlets

Sousa

Miranda

Freire

2011

Braz. arch. biol. technol.

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“…Altas concentrações de KIN exógena em culturas de calos, após três meses de cultivo, podem aumentar o conteúdo total de citocininas endógenas (Pintos et al, 2002). O TDZ pode atuar na proliferação de calos de duas maneiras: com efeito de citocinina, na proliferação de diferenciação celular ou com ação similar à auxina, atuando na indução da competência embriogênica (Singh et al, 2003).…”

Section: Associação De Diferentes Citocininas No Processo Calogênicounclassified

Efeito de diferentes meios nutritivos e fitorreguladores visando à otimização da calogênese de Annona mucosa (Jacq.)

Barboza

Lage

Moss

et al. 2014

Rev. bras. plantas med.

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Effect of different culture media and plant growth regulators for the optimization of callus induction of Annona mucosa (Jacq.). The Annona mucosa is a fruit tree of the Annonaceae family that produces a range of secondary metabolites of medicinal interest, such as lignans, acetogenins and alkaloids. The callus culture represents a renewable source of valuable medicinal compounds and controlled supply of material for biological and phytochemical studies. Therefore, this study was carried out to investigate the effects of three nutrient media, different concentrations of picloram and cytokinin types, in order to optimize the biomass yield and friability of calluses of A. mucosa. Leaf and hypocotyl segments from seedlings produced from in vivo seed germination were used as explants, which were inoculated in MS, WPM and B5 culture media supplemented with picloram (2-20µM) only or in addition to the cytokinins BAP, KIN or TDZ (0,2 -1μM ). Cultures were maintained at 26±2°C in the dark, with monthly subcultures. After 90 days, biomass production was evaluated. In all treatments, hypocotyl explants provided the highest percentage of callus formation, particularly in WPM. The association with TDZ produced highly proliferative friable callus, with no oxidation, reaching higher values than the previous works with this species. The results enable the use of the calluses produced in cell suspensions and the subsequent phytochemical characterization, in order to explore the in vitro production of metabolites of the species.

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Endogenous cytokinin levels in embryogenic and non-embryogenic calli of Medicago arborea L.

Cited by 23 publications

References 30 publications

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA) to detect the cytokinins in Gerbera jamesonii plantlets

Efeito de diferentes meios nutritivos e fitorreguladores visando à otimização da calogênese de Annona mucosa (Jacq.)

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