Endogenous Cell Therapy Improves Bone Healing

Layliev, John; Marchac, A; Tanaka, Rica; Szapalski, Caroline; Henderson, Raven; Rubin, Marcie S.; Saadeh, Pierre B.

doi:10.1097/scs.0000000000001306

Cited by 2 publications

(8 citation statements)

References 31 publications

(55 reference statements)

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“…In the present in vivo study using a rat tibial model, it was examined whether substances that enhance the recruitment of progenitor cells could be used as an approach to promote osseointegration of bone‐anchoring implants. Plerixafor is known to act as a CXCR4 antagonist, and hence mediate progenitor cell mobilization . Therapeutic treatments with plerixafor rely on the ability of the drug to mobilize stem cells and increase their availability.…”

Section: Discussionmentioning

confidence: 99%

“…Plerixafor is known to act as a CXCR4 antagonist, and hence mediate progenitor cell mobilization. [7][8][9]18 Therapeutic treatments with plerixafor rely on the ability of the drug to mobilize stem cells and increase their availability. SDF-1 is known as a chemokine that recruit stem cells to the site of healing.…”

Section: Discussionmentioning

confidence: 99%

“…The subsequent in vivo study using flow cytometry and bioluminence demonstrated that plerixafor mobilized cells from the BM to the circulation and provided prolonged circulation. Further studies in murine models and healthy human volunteers patients have demonstrated increased mobilization of hematopoietic stem and progenitor cells (HSPC) within a few hours of plerixafor administration . By this ability of plerixafor, an improved bone healing in calvarial defects in a mice model was obtained .…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9][10] By this ability of plerixafor, an improved bone healing in calvarial defects in a mice model was obtained. 7 A phase 2 trial demonstrated a significant enhancement of HSCs mobilization when plerixafor was combined with granulocyte colony-stimulating factor (G-CSF) in patients with non-Hodgkin lymphoma (NHL) and multiple myeloma. 11 Another approach to enhance tissue regeneration would be to create concentration gradients of chemokines to attract progenitor cells.…”

Section: Introductionmentioning

confidence: 99%

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Stem cell homing using local delivery of plerixafor and stromal derived growth factor‐1alpha for improved bone regeneration around Ti‐implants

Karlsson

Harmankaya

Palmquist

et al. 2016

J Biomedical Materials Res

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Triggering of the early healing events, including the recruitment of progenitor cells, has been suggested to promote bone regeneration. In implantology, local drug release technologies could provide an attractive approach to promote tissue regeneration. In this study, we targeted the chemotactic SDF-1α/CXCR4 axis that is responsible e.g. for the homing of stem cells to trauma sites. This was achieved by local delivery of plerixafor, an antagonist to CXCR4, and/or SDF-1α, from titanium implants coated with mesoporous titania thin films with a pore size of 7.5 nm. In vitro drug delivery experiments demonstrated that the mesoporous coating provided a high drug loading capacity and controlled release. The subsequent in vivo study in rat tibia showed beneficial effects with respect to bone-implant anchorage and bone-formation along the surface of the implants when plerixafor and SDF-1α were delivered locally. The effect was most prominent by the finding that the combination of the drugs significantly improved the mechanical bone anchorage. These observations suggest that titanium implants with local delivery of drugs for enhanced local recruitment of progenitor cells have the ability to promote osseointegration. This approach may provide a potential strategy for the development of novel implant treatments. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2466-2475, 2016.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stem cell homing using local delivery of plerixafor and stromal derived growth factor‐1alpha for improved bone regeneration around Ti‐implants

Karlsson

Harmankaya

Palmquist

et al. 2016

J Biomedical Materials Res

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“…Studies have shown that both VEGF and BMPs increase the differentiation of pre-osteoblasts and that they elicit a synergistic effect on bone formation. 13 – 18 …”

Section: Introductionmentioning

confidence: 99%

Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

2016

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Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. Research has demonstrated the potential of multipotent adult progenitor cells for treating ischemic injury and cardiovascular repair; however, understanding of multipotent adult progenitor cells in orthopedic applications remains limited. In this study, we evaluate the osteogenic and angiogenic capacity of multipotent adult progenitor cells, both in vitro and loaded onto demineralized bone matrix in vivo, with comparison to mesenchymal stem cells, as the current standard. When compared to mesenchymal stem cells, multipotent adult progenitor cells exhibited a more robust angiogenic protein release profile in vitro and developed more extensive vasculature within 2 weeks in vivo. The establishment of this vascular network is critical to the ossification process, as it allows nutrient exchange and provides an influx of osteoprogenitor cells to the wound site. In vitro assays confirmed the multipotency of multipotent adult progenitor cells along mesodermal lineages and demonstrated the enhanced expression of alkaline phosphatase and production of calcium-containing mineral deposits by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells demonstrated enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrix–only control groups. The potent combination of angiogenic and osteogenic properties provided by multipotent adult progenitor cells appears to create a synergistic amplification of the bone healing process. Our results indicate that multipotent adult progenitor cells have the potential to better promote tissue regeneration and healing and to be a functional cell source for use in orthopedic applications.

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Endogenous Cell Therapy Improves Bone Healing

Abstract: The combination of a PC-mobilizing and traffic-enhancing agent improved bony regeneration of calvarial defects in mice.

Cited by 2 publications

References 31 publications

Stem cell homing using local delivery of plerixafor and stromal derived growth factor‐1alpha for improved bone regeneration around Ti‐implants

Stem cell homing using local delivery of plerixafor and stromal derived growth factor‐1alpha for improved bone regeneration around Ti‐implants

Multipotent adult progenitor cells on an allograft scaffold facilitate the bone repair process

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