Endogenous bioactive gibberellin/abscisic acids and enzyme activity synergistically promote the phytoremediation of alkaline soil by broomcorn millet (Panicum miliaceum L.)

Ma, Qian; Yuan, Yuhao; Wu, Enguo; Wang, Honglu; Dang, Ke; Feng, Yu; Ivanistau, Aliaksandr; Feng, Baili

doi:10.1016/j.jenvman.2021.114362

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…In the GA pathway, the expression of the three GA2ox genes that regulate GA 4 breakdown was significantly inhibited during maturation, and the expression of the CYP701 gene was significantly upregulated after treatment, indicating that GA2ox and CYP701 cooperate to promote the massive accumulation of GA 7 and GA 3 . In the study of alkaline stress, the authors found that alkaline stress also changed the content of endogenous GA 3 by changing the expression of GA2ox ( Ma et al., 2022 ). Bermejo et al.…”

Section: Discussionmentioning

confidence: 99%

Joint metabolome and transcriptome analysis of the effects of exogenous GA3 on endogenous hormones in sweet cherry and mining of potential regulatory genes

Chen

et al. 2022

Front. Plant Sci.

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Gibberellin (GA) is an important phytohormone that can participate in various developmental processes of plants. The study found that application of GA3 can induce parthenocarpy fruit and improve fruit set. However, the use of GA3 affects endogenous hormones in fruits, thereby affecting fruit quality. This study mainly investigates the effect of exogenous GA3 on endogenous hormones in sweet cherries. The anabolic pathways of each hormone were analyzed by metabolome and transcriptome to identify key metabolites and genes that affect endogenous hormones in response to exogenous GA3 application. Results showed that exogenous GA3 led to a significant increase in the content of abscisic acid (ABA) and GA and affected jasmonic acid (JA) and auxin (IAA). At the same time, the key structural genes affecting the synthesis of various hormones were preliminarily determined. Combined with transcription factor family analysis, WRKY genes were found to be more sensitive to the use of exogenous GA3, especially the genes belonging to Group III (PaWRKY16, PaWRKY21, PaWRKY38, PaWRKY52, and PaWRKY53). These transcription factors can combine with the promoters of NCED, YUCCA, and other genes to regulate the content of endogenous hormones. These findings lay the foundation for the preliminary determination of the mechanism of GA3’s effect on endogenous hormones in sweet cherry and the biological function of WRKY transcription factors.

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Section: Discussionmentioning

confidence: 99%

Joint metabolome and transcriptome analysis of the effects of exogenous GA3 on endogenous hormones in sweet cherry and mining of potential regulatory genes

Chen

et al. 2022

Front. Plant Sci.

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“…Currently, most of the existing studies have focused on broomcorn millet under low nitrogen, drought, and neutral salt stress (Liu et al, 2020; Yuan, Li, et al, 2021; Yuan, Liu, et al, 2021) rather than alkaline stress. The research on alkaline resistance of broomcorn millet is still at the stage of alkaline‐tolerant resource screening (Ma et al, 2021) and physiological and biochemical responses in germination (Ma et al, 2022). Additionally, breeding is a long‐term and challenging project.…”

Section: Introductionmentioning

confidence: 99%

“…Broomcorn millet (Panicum miliaceum L.) is one of the earliest domesticated cereal/bioenergy crops in the world (Ma et al, 2021;Yuan et al, 2022;Zou et al, 2019). In the northern China, broomcorn millet is an indispensable food crop and forage grass.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and metabolomic study reveal that exogenous 24‐epiandrosterone mitigate alkaline stress in broomcorn millet (Panicum miliaceum L.) via regulating photosynthesis and antioxidant capacity

Wang

et al. 2023

GCB Bioenergy

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Globally, land alkalinization affecting agricultural development. Considering the increasingly serious effects of alkaline stress on agriculture and environment, phytoremediation may be an efficient way to addressed land alkalinization. Broomcorn millet (Panicum miliaceum L.) is a saline-alkaline-tolerant crop and bioenergy crop. However, the molecular mechanism of alkaline response on broomcorn millet remains large gap. To explore the alkaline stress on broomcorn millet and the mitigation of 24-epicastasterone (BR), the effects of CK (nutrient solution only), CB (nutrient solution + 0.5 mg L −1 BR), AS (alkaline stress), and AB (alkaline stress + 0.5 mg L −1 BR) on TA289 (alkaline-tolerant) and SA223 (alkaline-sensitive) were investigated. Alkaline stress enhanced reactive oxygen species and membrane lipid peroxidation. BR boosted antioxidant enzyme activities to reduce oxidative stress. Simultaneously, BR attenuated Na + toxicity and maintained ion homeostasis. Additionally, BR significantly maintained the physiological structure and photosynthetic properties. Transcriptomic and metabolomic analyses were applied to further evaluate the effect of BR on photosynthetic and antioxidant defense. The results showed that BR significantly reduced the transcriptional responses of photosynthesis and antioxidant defense and promoted the accumulation of effective metabolites such as biliverdin, l-glutamate, and phosphoric acid. Taken together, BR application can significantly alleviate the damage of alkaline stress to broomcorn millet by altering transcriptional expression and metabolite accumulation and is a simple and effective strategy to alleviate alkaline stress. This study reveals the molecular mechanism of BR to enhance photosynthetic capacity and antioxidant defense of broomcorn millet under alkaline stress, which provides theoretical support for the cultivation of bioenergy crops on alkaline lands and the breeding of alkaline-tolerant bioenergy varieties.

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“…Studies based on the plant growth, physiological, and biochemical changes under alkaline stress have included wheat (Guo et al, 2010), apple (Liu, Jin, et al, 2021), tomato (Gong et al, 2014), and alfalfa (Wang, Wang, et al, 2021). Increases in Na + concentration and pH accompanying alkaline stress may have many adverse effects on plant growth, including seed germination, morphogenesis, and organ development, thereby inhibiting crop yield (Ma et al, 2021, 2022). In addition, excess reactive oxygen species (ROS) accumulate under alkaline stress, leading to oxidative stress, malondialdehyde (MDA) accumulation, and membrane structure damage, increasing the blockage of phytochrome synthesis and reducing the photosynthetic performance (Liu, Jin, et al, 2021; Ma et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Alkaline stress inhibits the germination rate of broomcorn millet and adversely affects its root length, shoot length, and photosynthesis (Ma et al, 2021). In addition, gibberellin/abscisic acid and enzyme activities of the seeds coordinately promote broomcorn millet germination in alkaline treatments (Ma et al, 2022). The restoration of damaged land through biological processes incurs high human and financial costs.…”

mentioning

confidence: 99%

Comprehensive physiological, transcriptomic, and metabolomic analysis of the response of Panicum miliaceum L. roots to alkaline stress

Wang

et al. 2023

Land Degrad Dev

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Alkaline stress disrupts transcriptional expression and causes metabolite accumulation, thus affecting plant growth. However, there are gaps in the response mechanism of plants to alkaline stress at the molecular dimension. Broomcorn millet (Panicum miliaceum L.) is a pioneer plant for stress resistance also is a future smart food crop. To explore the physiological‐molecular response and adaptation mechanism of broomcorn millet root to alkaline stress, we conducted physiological, transcriptomic, and metabolomic analyses on roots of two broomcorn millet cultivars (alkaline‐sensitive S223 and alkaline‐tolerant T289) that were exposed to normal conditions (CK) and alkaline stress (AS) treatments (40 mM and a molar ratio of Na2CO3: NaHCO3 = 1:9) for 7 days. Alkaline stress inhibited mineral uptake in broomcorn millet roots and enhanced the antioxidant enzyme activities, malondialdehyde, and soluble substances, resulting in changes in growth, biomass, and root structure. Correspondingly, differentially expressed genes induced by alkaline stress were prominently enriched in the plant hormone signal transduction, mitogen‐activated protein kinase (MAPK) signaling, phenylpropanoid biosynthesis, ATP binding cassette (ABC) transporters, and other biological pathways. Similar results were obtained from the differentially accumulated metabolites analysis. In addition, joint transcriptome and metabolome analysis indicated that genes of phenylpropanoid and flavonoid biosynthesis pathways were upregulated or downregulated in AS groups, however, the related metabolite accumulations were inhibited. This study integrates multiple methods to uncover the mechanisms of broomcorn millet response to alkaline stress. These findings suggest a new direction for phytoremediation of alkaline lands.

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Endogenous bioactive gibberellin/abscisic acids and enzyme activity synergistically promote the phytoremediation of alkaline soil by broomcorn millet (Panicum miliaceum L.)

Cited by 7 publications

References 33 publications

Joint metabolome and transcriptome analysis of the effects of exogenous GA3 on endogenous hormones in sweet cherry and mining of potential regulatory genes

Joint metabolome and transcriptome analysis of the effects of exogenous GA3 on endogenous hormones in sweet cherry and mining of potential regulatory genes

Comparative transcriptomic and metabolomic study reveal that exogenous 24‐epiandrosterone mitigate alkaline stress in broomcorn millet (Panicum miliaceum L.) via regulating photosynthesis and antioxidant capacity

Comprehensive physiological, transcriptomic, and metabolomic analysis of the response of Panicum miliaceum L. roots to alkaline stress

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