Endogenous and exogenous citrate transport and release in prostatic preparations: semi-polarized two-dimensional cultures of human PNT2-C2 cells and isolated tubules and segments of rat prostate

Mycielska, Maria E.; Krasowska, Monika; Grzywna, Zbigniew J.; Djamgoz, Mustafa B.A.

doi:10.1002/pros.20274

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…Short‐circuit current ( I sc ) of tissue segments was measured in an Ussing chamber maintained at 37 °C and gassed with 95% O 2 /5% CO 2 as described (Liao et al ., ). Rat prostatic segments have been used before for the study of I sc (Mycielska et al ., ). In brief, the tissue was mounted on a silicon ring with 0.2 cm 2 area, clamped vertically between two halves of an Ussing chamber and short circuited with the transepithelial potential difference clamped at zero by a voltage‐clamp amplifier (DVC‐1000; World Precision Instruments, New Haven, CT, USA).…”

Section: Methodsmentioning

confidence: 97%

Adrenomedullin increases the short‐circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion

Liao

Cheung

et al. 2014

Andrology

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SUMMARYIn this study, we have investigated the effects of adrenomedullin on chloride and fluid secretion in the rat prostate. The presence of adrenomedullin (ADM) in rat prostate was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with an enzyme-linked assay for ADM. The effects of ADM on fluid secretion were studied by short-circuit current technique in a whole mount preparation of the prostate in an Ussing chamber. The results indicated that the ADM level was higher in the ventral than the dorso-lateral prostate and the major molecular species was the active peptide. ADM increased the short-circuit current through both the cAMP-and calcium-activated chloride channels in the ventral lobe, but only through the calcium-activated channels in the dorso-lateral lobe. These stimulatory effects were blocked by the calcitonin generelated peptide (CGRP) receptor antagonist, hCGRP8-37. We conclude that ADM may regulate prostatic fluid secretion through the chloride channels, which may affect the composition of the seminal plasma bathing the spermatozoa and hence fertility.

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Section: Methodsmentioning

confidence: 97%

Adrenomedullin increases the short‐circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion

Liao

Cheung

et al. 2014

Andrology

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“…In the beginning, this model was used in the investigations of carcinogenesis from a hormonal perspective, but now it is still practiced as a useful model for investigations of androgen-independent growth of prostatic adenocarcinoma cells and the molecular basis of metastases. Though Dunning's rat prostatic cancer model is one of the first ones that was accepted, it is still widely used, having many subcellular strains, grown both in vivo and in vitro (75)(76)(77)(78). Prostatic cancer is formed by the inoculation of highly metastatic Mat-LyLu cells from these strains into Copenhagen rats and metastasizes to the lungs and the lymphatic system (76,79,80).…”

Section: Animal Models Of Prostate Cancermentioning

confidence: 99%

New Insight Into Metformin Action: Diabetes, Prostate Cancer, Ion Channels

Karabulut-Bulan¹

2016

Marmara Pharm J

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Diabetes Mellitus is a life-long chronic metabolic disease, requiring continuous follow-up and therapy, it reduces the quality of life of patients with acute and chronic complications, mortality, and its economic burden is high. Cancer is the second cause of death, according to the data from World Health Organization. Prostate cancer is one of the most common cancers in the developed world and the second leading cause of male cancer-related death. As with the other cancer types, metastasis is an essential problem that we are facing and it is not clear whether a tumor will metastasize or not in localized state. It has been reported that there are high levels of voltage-gated sodium channels in metastatic prostate cancer cases. Cancer, ever growing with diabetes, is a major health problem. Studies have shown that diabetic patients have higher cancer rates than those of non-diabetics. Metformin is the drug of choice for the treatment of diabetes. Recently, there are studies in the literature regarding metformin reducing the risk of cancer besides its effect on diabetes This review will explain the possible role of the metformın on the three dimensional relationship of prostate cancer, diabetes and ion channels, and provide a significant contribution to clinical trials.

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“…(34) This mechanism is designed primarily to transport citrate in outward direction. (34,35) This putative transporter has been called 'KCitT1' (K þ -dependent citrate transport mechanism) (Figs. 2a).…”

Section: Cellular Physiology Of Citrate In Prostatementioning

confidence: 99%

“…The proposed ionic cycling is consistent with the electrolytic characteristics of blood and prostatic fluid. The data have been integrated from published works (31,34,35,38,45) . A1, Na þ /K þ -ATPase.…”

Section: System Organization and Ionic Cyclingmentioning

confidence: 99%

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Citrate transport and metabolism in mammalian cells

et al. 2009

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Citrate, an organic trivalent anion, is a major substrate for generation of energy in most cells. It is produced in mitochondria and used either in the Krebs' cycle or released into cytoplasm through a specific mitochondrial carriers. Citrate can also be taken up from blood through different plasma membrane transporters. In the cytoplasm, citrate can be used ultimately for fatty acid synthesis, which is increased in cancer cells. Here, we review the ways in which citrate can be transported and discuss the changes in transport and metabolism that occur in cancer cells. The primary focus is on the prostate gland, which is known to produce and release large amounts of citrate during its normal secretory function. The significant changes that occur in citrate-related metabolism and transport in prostate cancer are the second focus. This review strives to relate these mechanisms to molecular biology on the one hand and to clinical applications on the other.

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Endogenous and exogenous citrate transport and release in prostatic preparations: semi-polarized two-dimensional cultures of human PNT2-C2 cells and isolated tubules and segments of rat prostate

Abstract: Citrate release from prostatic cells and tissues (rat and human) was K(+)-dependent, consistent with the previous electrophysiological data.

Cited by 5 publications

References 36 publications

Adrenomedullin increases the short‐circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion

Adrenomedullin increases the short‐circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion

New Insight Into Metformin Action: Diabetes, Prostate Cancer, Ion Channels

Citrate transport and metabolism in mammalian cells

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