Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring

Role of Trichloroethylene in Parkinson’s Disease

“…Like MPTP, it freely crosses the BBB , and it specifi cally inhibits mitochondrial Complex 1-though TaClo and its metabolites are at least an order of magnitude more potent (Janetzky et al 1995 ;Bringmann et al 1995 ). TaClo is a dopaminergic toxin both in culture and in vivo Bringmann et al 1995Bringmann et al , 1996Riederer et al 2002 ;Heim and Sontag 1997 ;Grote et al 1995 ). Injection of TaClo into the SNpc of rats resulted in a 15 % decrease in neuronal count .…”

Identification of the Dopaminergic Neurotoxin 1-Trichloromethyl-1,2,3,4-tetrahydro-β-carboline in Human Blood after Intake of the Hypnotic Chloral Hydrate

“…After centrifugation (30 min), the clear sample solution was carefully transferred to a new vial. After addition of 50 l of 0.1 M sodium hydroxide, further sample workup was accomplished subsequently on a 100-mg sorbent C 18 cartridge (cleanup, C18 octadecyl encapped, United Chemical Technologies Inc.) according to a procedure, the reliability of which has been outlined in detail previously (18). Prior to solid-phase extraction, the cartridge was conditioned with 2 ml of methanol followed by 3 ml of water.…”

“…We wish to emphasize particularly that every GC run of a purified human blood sample was performed after several injections of pure toluene to really avoid any memory effects on the capillary column used. First, we focused on the investigation of the clot fractions because former studies on blood samples of TaClo-treated rats had revealed this substance to mainly occur in the fraction of lysed erythrocytes (5,6,18), presumably due to the unpolar character of the TaClo molecule. Indeed, the chromatograms (Fig.…”