Endodontic regeneration: hard shell, soft core

Widbiller, Matthias; Schmalz, Gottfried

doi:10.1007/s10266-020-00573-1

Cited by 27 publications

(40 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite in vivo evidence and promising case reports, the translation of in situ tissue engineering into humans seems still unattained. Similarly, the regeneration of the dentin pulp complex as a whole owing to the interconnection of its components [ 178 ], albeit theoretically feasible [ 179 ], is hardly implementable at a clinical level to date.…”

Section: Mscs-based Therapeutic Approachesmentioning

confidence: 99%

Oral Cavity as a Source of Mesenchymal Stem Cells Useful for Regenerative Medicine in Dentistry

et al. 2021

View full text Add to dashboard Cite

The use of mesenchymal stem cells (MSCs) for regenerative purposes has become common in a large variety of diseases. In the dental and maxillofacial field, there are emerging clinical needs that could benefit from MSC-based therapeutic approaches. Even though MSCs can be isolated from different tissues, such as bone marrow, adipose tissue, etc., and are known for their multilineage differentiation, their different anatomical origin can affect the capability to differentiate into a specific tissue. For instance, MSCs isolated from the oral cavity might be more effective than adipose-derived stem cells (ASCs) for the treatment of dental defects. Indeed, in the oral cavity, there are different sources of MSCs that have been individually proposed as promising candidates for tissue engineering protocols. The therapeutic strategy based on MSCs can be direct, by using cells as components of the tissue to be regenerated, or indirect, aimed at delivering local growth factors, cytokines, and chemokines produced by the MSCs. Here, the authors outline the major sources of mesenchymal stem cells attainable from the oral cavity and discuss their possible usage in some of the most compelling therapeutic frontiers, such as periodontal disease and dental pulp regeneration.

show abstract

Section: Mscs-based Therapeutic Approachesmentioning

confidence: 99%

Oral Cavity as a Source of Mesenchymal Stem Cells Useful for Regenerative Medicine in Dentistry

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Anatomically the dental pulp, although populated by terminally differentiated cells, including odontoblasts, also contains a resident population of adult stem as well as migratory progenitor cell populations (11) that can contribute to reparative processes, consequently a combination of replacement and regeneration could occur in the diseased and damaged pulp. The terms repair and regeneration have generally been used interchangeably when describing the nature of pulp wound healing process (12). In reality, however true regeneration and reconstitution of the native tissues is seldom achieved after damage to dentin-pulp complex using current clinically-approved treatment modalities, therefore the pulpal healing response is most appropriately described as a repair.…”

Section: The Process Of Tissue Repair In Pulpitismentioning

confidence: 99%

Deciphering Reparative Processes in the Inflamed Dental Pulp

et al. 2021

View full text Add to dashboard Cite

Research over several decades has increased our understanding of the nature of reparative and regenerative processes in the dental pulp, at both the cellular and molecular level. However, advances in scientific knowledge have not translated into novel clinical treatment strategies for caries-induced pulpitis. This narrative review explores the evidence regarding the ability of inflamed pulp tissue to heal and how this knowledge may be used therapeutically. A literature search and evidence analysis covering basic, translational and clinical pulp biology research was performed. The review focuses on (1) the regenerative and defense capabilities of the pulp during caries-induced inflammation; (2) the potential of novel biomaterials to harness the reparative and regenerative functions of the inflamed pulp; and (3) future perspectives and opportunities for conservative management of the inflamed pulp. Current conservative management strategies for pulpitis are limited by a combination of unreliable diagnostic tools and an outdated understanding of pulpal pathophysiological responses. This approach leads to the often unnecessary removal of the entire pulp. Consequently, there is a need for better diagnostic approaches and a focus on minimally-invasive treatments utilizing biologically-based regenerative materials and technologies.

show abstract

“…Therefore, cell homing has been advocated as a potential strategy and feasible alternative [ 3 , 4 ]. Cell homing does not rely on cell transplantation but aims at the recruitment of local stem cells into a custom-made scaffold material laden with bioactive molecules that are inserted into the defect or site of injury [ 5 , 6 ]. The presence of mesenchymal stem cells in the apical region has been demonstrated, along with their potential to differentiate into pulp- and dentin-forming cells [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Endogenous TGF-β1 from Root Canals for Pulp Tissue Engineering: A Translational Study

Widbiller

Rosendahl

Wölflick

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

Cell homing for dental pulp tissue engineering has been advocated as a feasible approach to regenerate dental pulp in a clinical setting. In order to develop a translational protocol for clinical application, we wanted to determine the effects of disinfectants on the availability of growth factors from the root canal, the amount that can be obtained in this context, and whether they can be processed for use in tissue engineering procedures. The extraction of growth factors should also be confirmed in a clinical setting. Root canals were prepared in 36 extracted mature teeth, and the amount of TGF-β1 in solution was quantified after different irrigation protocols (sodium hypochlorite, chlorhexidine) and after intracanal medication (calcium hydroxide). Centrifugal filters with a cut-off of 10,000 Da and 3000 Da were used for efficient concentration, and volumes and amounts of retained TGF-β1 were measured at different time points. During conventional endodontic treatment, ethylenediaminotetraacetic acid (EDTA) solution was collected after ultrasonic activation from the root canals of mature teeth of 38 patients, and growth factor content was quantified via enzyme-linked immunosorbent assay (ELISA). Irrigation with sodium hypochlorite reduced TGF-β1 release into EDTA. This effect was partially reversed by canal enlargement after the use of sodium hypochlorite and by subsequent use of calcium hydroxide. A few minutes of centrifugation with a cut-off of 10,000 Da reduced the initial volume of the irrigant by 90% and led to a continuous increase in concentration to the same extent. Furthermore, TGF-β1 was obtained from root canals of mature teeth during endodontic treatment in quantities that have been shown to elicit desirable cellular responses in a subsequent clinical application. A mixture with a suitable scaffold material and injection into the root canal has the potential to promote dental pulp regeneration.

show abstract

Endodontic regeneration: hard shell, soft core

Cited by 27 publications

References 87 publications

Oral Cavity as a Source of Mesenchymal Stem Cells Useful for Regenerative Medicine in Dentistry

Oral Cavity as a Source of Mesenchymal Stem Cells Useful for Regenerative Medicine in Dentistry

Deciphering Reparative Processes in the Inflamed Dental Pulp

Isolation of Endogenous TGF-β1 from Root Canals for Pulp Tissue Engineering: A Translational Study

Contact Info

Product

Resources

About