Endodontic infections increase leukocyte and lymphocyte levels in the blood

Samuel, Renata Oliveira; Gomes‐Filho, João Eduardo; Azuma, Mariane Maffei; Sumida, Dóris Hissako; Oliveira, Sandra Helena Penha de; Chiba, Fernando Yamamoto; Bomfim, Suely Regina Mogami; Ciarlini, Paulo César; Narciso, Luı́s; Cintra, Luciano Tavares Ângelo

doi:10.1007/s00784-017-2222-z

Cited by 20 publications

(23 citation statements)

References 42 publications

(65 reference statements)

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“…Samuel et al . () demonstrated that the presence of apical periodontitis increased cytokine levels in blood. However, in both studies, researchers found this alteration was significant only in multiple apical periodontitis groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Aksoy¹,

Savtekin

Şehirli

et al. 2019

Int Endodontic J

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Aim To investigate the possible therapeutic effects of alpha‐lipoic acid (ALA) in a model of chronic apical periodontitis in rats by analysing biochemical, histopathological and micro‐CT parameters. Methodology The study was approved by the Animal Ethics Committee of the Near East University. Thirty‐two Wistar rats were divided into four groups of eight rats each: Control Group; ALA Group; AP Group; AP + ALA Group. In the AP and AP + ALA groups, the pulp chambers of the mandibular first molars were surgically exposed and were left open to the oral environment for 4‐weeks to allow the establishment of periapical lesions. The rats in the Control and AP groups were treated intraperitoneally with saline solution (with a daily dose of 100 mg kg−1, for 28 days after periapical lesion induction). The rats in the ALA and AP + ALA groups were treated intraperitoneally with ALA (with a daily dose of 100 mg kg−1, for 28 days after periapical lesion induction). After decapitation, the trunk blood was collected for the assessment of biochemical parameters. The mandibles were surgically removed and dissected for histopathologic analysis and further scanned with micro‐CT. Groups of data were compared with a two‐way analysis of variance (two‐way anova) followed by Sidak's multiple comparison tests. Values of P < 0.05 were regarded as significant. Results TNF‐α, IL‐1β, MMP‐1, MMP‐2 levels were significantly lower in AP + ALA group compared with AP group (P < 0.05). There was a significant difference between the AP and AP + ALA groups according to assessment of the inflammatory scores (P < 0.05). The periapical inflammatory infiltrates were significantly more severe (P < 0.05) in the AP group. The AP + ALA group exhibited lower values both in terms of surface area and volume of resorption cavities than the AP group and this difference was significant (P < 0.05). Conclusion alpha‐lipoic acid treatment provided therapeutic effects on the inhibition of periapical bone loss.

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Section: Discussionmentioning

confidence: 99%

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Aksoy¹,

Savtekin

Şehirli

et al. 2019

Int Endodontic J

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“…Experimental induction of AP in rats is a reliable and a generally accepted model for studying AP. The use of an experimental animal model allowed us to carry out the research under standardized conditions in which rats of the same strain, sex, and weight received the same food, experienced the same stress, and shared the same housing facilities .…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study by Samuel et al . , it was demonstrated that the presence of AP stimulates elevation of cytokine levels in blood. However, in both studies, researchers found this alteration to be significant only in the presence of more than one tooth with AP.…”

Section: Discussionmentioning

confidence: 99%

“…Previous animal studies on rats have demonstrated that AP affects blood homeostasis; it increases the serum levels of inflammatory mediators and thus can affect systemic health . Alterations in expression of inflammatory markers, such as creatine kinase (CK), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), and in the activities of antioxidant enzymes, such as superoxide dismutase (SOD), have previously been studied in cardiovascular diseases and marginal periodontitis .…”

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confidence: 99%

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Protective effect of alpha‐lipoic acid against apical periodontitis‐induced cardiac injury in rats

Şehirli¹,

Aksoy²,

Basmacı³

et al. 2019

European J Oral Sciences

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This study was designed to assess the possible protective effect of alpha‐lipoic acid (ALA) on apical periodontitis (AP)‐induced cardiac injury. Wistar albino rats were randomized into four groups: control; ALA; AP; and ALA + AP. Rats of the control and ALA groups were not endodontically treated, but saline and ALA (100 mg kg−1) were administered. In rats of the AP and ALA + AP groups, the pulp chambers of mandibular first molar teeth were exposed and left open for 30 d to induce AP. Saline and ALA (100 mg kg−1) were administered intraperitoneally every 24 h during the experiment. At the end of the experiment, the rats were killed. Establishment of AP was verified by radiographic and histopathological evaluation. Serum alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), and superoxide dismutase (SOD) activities were determined using an automated biochemical analyzer, and the structural cardiac injury was assessed pathologically. Serum ALP, LDH, and CK activities were elevated, and SOD activities were decreased, in the AP group. The changed enzyme activities were significantly normalized by treatment with ALA. We conclude that ALA administration alleviated the AP‐induced heart injury and improved cardiac structure and function, and therefore this agent may be of potential therapeutic value in protecting cardiac tissue from systemic injury caused by AP.

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“…The following parameters of the complete blood count were analysed using an automatic analyser (ABX Micros ABC Vet; Horiba ABX Diagnostics, Montpellier, France): haemoglobin, haematocrit, mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), fibrinogen, total leucocytes, neutrophils, lymphocytes, eosinophils and monocytes (Samuel et al 2018). The samples were placed in EDTA, homogenized and immediately transferred to a technician, who was blinded to the case status, for processing.…”

Section: Blood Sample Collectionmentioning

confidence: 99%

Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

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Aim To evaluate the effect of systemic administration of probiotics on the severity of apical periodontitis (AP). Methodology Twenty‐four male Wistar rats were used. AP was induced in the maxillary left/right first molars. The animals were arranged into groups: Control, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Probiotics were administered orally for gavage (109 colony‐forming units diluted in 5 mL of water for 30 days) during the development of AP. After 30 days, cardiac puncture was performed to analyse the complete blood count. Moreover, microbiological analysis of the root canal contents and saliva was performed. Then, the animals were euthanized and the jaw removed for histopathological and IL‐10, IL‐1β and IL‐6 immunolabeling analyses. After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn's test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05). Results No significance difference was observed in the blood profiles and in the counts of microorganisms from the saliva samples among the groups (P > 0.05). Total microorganism counts in the root canal, the inflammatory infiltrate and the immunostaining for IL‐1β and IL‐6 in AP were significantly lower in the probiotic groups when compared with the control group (P < 0.05). IL‐10 was significantly more immunolabled in the probiotic groups than in the control group (P < 0.05). Conclusion Supplementation with probiotics (Lactobacillus rhamnosus and Lactobacillus acidophilus) had a significant effect on the severity of apical periodontitis in rats, demonstrating the anti‐inflammatory effect of probiotics on the development of apical periodontitis.

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Endodontic infections increase leukocyte and lymphocyte levels in the blood

Abstract: The presence of endodontic infections can interfere with the blood profile, altering systemic health.

Cited by 20 publications

References 42 publications

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

Protective effect of alpha‐lipoic acid against apical periodontitis‐induced cardiac injury in rats

Systemic administration of probiotics reduces the severity of apical periodontitis

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