Introduction: Irrigation of the root canals is an important step during endodontic treatment. Irrigants serve to eradicate microbial infection and to remove the resulting smear layer. NaOCl/EDTA irrigation is the gold standard irrigation protocol. However, it has several side effects. So, it was urgent call to investigate herbal alternatives as potential endodontic irrigants. Aim: To evaluate the antimicrobial action and smear layer removal ability of apple, dates and pomegranate vinegars compared to NaOCl/ EDTA irrigation. Materials &Methods: Sixty-five sterile roots were infected with E.faecalis, incubated for three weeks then grouped into five groups; apple vinegar group, dates vinegar group, pomegranate vinegar group, NaOCl/EDTA group and control group, respectively. Three Microbial samples were obtained from the root canals: before irrigation, immediately after irrigation and one week after irrigation. The reduction% of mean Log10 CFU was calculated. Five roots from each group were split bucco-lingually, scanned with SEM and processed using ImageJ software. Statistical analysis was performed by applying the t-test, ANOVA (one and two way) followed by post hoc Tukey's test at 95% significance level. Results: Dates vinegar group showed high antimicrobial action and smear layer removal ability comparable to NaOCl/EDTA group and the difference was statistically insignificant. Pomegranate and apple vinegar groups showed less antimicrobial action and smear layer removal ability than dates vinegar and NaOCl/EDTA which was statistically significant. Conclusion: Phenolic compounds in dates vinegar are responsible for its antimicrobial action and smear layer removal ability making it potential alternative to NaOCl/EDTA.