Endocytosis of tight junction proteins and the regulation of degradation and recycling

Stamatovic, Svetlana M.; Johnson, Allison M.; Sladojević, Nikola; Keep, Richard F.; Andjelkovic, Anuska V.

doi:10.1111/nyas.13346

Cited by 83 publications

(72 citation statements)

References 117 publications

(297 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the underlying intestinal layers may not appear exposed to luminal contents since the mucosa is no longer denuded, the unrestricted permeability pathway via poorly formed tight junctions allows for microorganisms and macromolecules to cross the epithelial barrier. In order for the tight junction barrier and cell polarity to be restored, tight junction proteins internalized during injury, such as the endocytosis of occludin that accompanies anoxic injury in Caco-2 cells, must be reinserted back into the membrane via recycling endosomes [51][52][53]. Ultimately, following these acute repair mechanisms, crypt cells can proliferate and differentiate to restore the proper number of epithelial cells to the monolayer in order to regain full homeostatic function.…”

Section: Acute Mechanisms Of Repair In Injured Intestinal Epitheliummentioning

confidence: 99%

The Integral Role of Tight Junction Proteins in the Repair of Injured Intestinal Epithelium

Slifer

Blikslager

2020

IJMS

142

View full text Add to dashboard Cite

The intestinal epithelial monolayer forms a transcellular and paracellular barrier that separates luminal contents from the interstitium. The paracellular barrier consists of a highly organized complex of intercellular junctions that is primarily regulated by apical tight junction proteins and tight junction-associated proteins. This homeostatic barrier can be lost through a multitude of injurious events that cause the disruption of the tight junction complex. Acute repair after injury leading to the reestablishment of the tight junction barrier is crucial for the return of both barrier function as well as other cellular functions, including water regulation and nutrient absorption. This review provides an overview of the tight junction complex components and how they link to other plasmalemmal proteins, such as ion channels and transporters, to induce tight junction closure during repair of acute injury. Understanding the components of interepithelial tight junctions and the mechanisms of tight junction regulation after injury is crucial for developing future therapeutic targets for patients experiencing dysregulated intestinal permeability.

show abstract

Section: Acute Mechanisms Of Repair In Injured Intestinal Epitheliummentioning

confidence: 99%

The Integral Role of Tight Junction Proteins in the Repair of Injured Intestinal Epithelium

Slifer

Blikslager

2020

IJMS

142

View full text Add to dashboard Cite

show abstract

“…The unique pinocytosis process of forming membrane extension or ru es as a result of cytoskeleton reorganization is macropinocytosis. As a consequence of these membrane changes the large amount of extracellular fluid with dissolved molecules is collected, regardless of the presence of any specific receptors [68,69]. Luther et al showed that Fe NPs were successfully taken up by microglial cells chiefly through macropinocytosis and clathrin-mediated endocytosis, in the course of which absorbed particles were directed into the lysosomal compartment [70].…”

Section: Cellular Uptakementioning

confidence: 99%

Toxicity of metallic nanoparticles in the central nervous system

Sawicki

Czajka

Matysiak‐Kucharek

et al. 2019

Nanotechnology Reviews

View full text Add to dashboard Cite

Metallic nanoparticles due to their small size and unique physico-chemical characteristics have found excellent applications in various branches of industry and medicine. Therefore, for many years a growing interest has been observed among the scientific community in the improvement of our understanding of the impact of nanoparticles on the living organisms, especially on humans. Considering the delicate structure of the central nervous systemit is one of the organs most vulnerable to the adverse effects of metallic nanoparticles. For that reason, it is important to identify the modes of exposure and understand the mechanisms of the effect of nanoparticles on neuronal tissue. In this review, an attempt is undertaken to present current knowledge about metallic nanoparticles neurotoxicity based on the selected scientific publications. The route of entry of nanoparticles is described, as well as their distribution, penetration through the cell membrane and the blood-brain barrier. In addition, a study on the neurotoxicity in vitro and in vivo is presented, as well as some of the mechanisms that may be responsible for the negative effects of metallic nanoparticles on the central nervous system. Graphical abstract: This review summarizes the current knowledge on the toxicity of metallic NPs in the brain and central nervous system of the higher vertebrates.

show abstract

“…However, on the basis of other reports, we have a few speculations or theories. First, internalization or endocytosis of TJ molecules [32]-the mechanisms of endocytosis and triggered signaling pathways following endocytosis are rather complicated. The process of endocytosis results in changes to the barrier function and plasticity of TJ molecules.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression and Function of Bicellular Tight Junctions in Skin and Oral Wound Healing

Leonardo

Shi

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

Bicellular tight junctions are multiprotein complexes that are required for maintenance of barrier function and fence function in epithelial tissues. Wound healing in the oral cavity leads to minimal scar formation compared to the skin, and the precise mechanisms for this regenerative response remain to be elucidated. We hypothesized that oral and skin tissues express a different tight junction repertoire both at baseline and during the wound healing response, and that these molecules may be critical to the differential repair between the two tissues. We re-analyzed a mouse skin and palate epithelium microarray dataset to identify the tight junction repertoire of these tissue types. We then re-analyzed a skin and tongue wound healing microarray dataset to see how expression levels of tight junction genes change over time in response to injury. We performed in vitro scratch assays on human oral and skin keratinocyte cell lines to assay for tight junction expression over time, tight junction expression in response to lipopolysaccharide and histamine treatment, and the effects of siRNA knockdown of claudin 1 or occludin on migration and proliferation. Our data showed that oral and skin epithelium expressed different tight junction genes at baseline and during the wound healing response. Knockdown of claudin 1 or occludin led to changes in proliferation and migration in human skin keratinocytes but not oral keratinocytes. Furthermore, we also showed that skin keratinocytes were more permeable than oral keratinocytes upon histamine treatment. In conclusion, this study highlights a specific subset of functional tight junction genes that are differentially expressed between the oral and skin tissues, which may contribute to the mechanisms leading to distinct healing phenotypes in response to injury in the two tissues.

show abstract

Endocytosis of tight junction proteins and the regulation of degradation and recycling

Cited by 83 publications

References 117 publications

The Integral Role of Tight Junction Proteins in the Repair of Injured Intestinal Epithelium

The Integral Role of Tight Junction Proteins in the Repair of Injured Intestinal Epithelium

Toxicity of metallic nanoparticles in the central nervous system

Differential Expression and Function of Bicellular Tight Junctions in Skin and Oral Wound Healing

Contact Info

Product

Resources

About