Endocytosis is a significant contributor to uranium(VI) uptake in tobacco (Nicotiana tabacum) BY-2 cells in phosphate-deficient culture

John, Warren A.; Lückel, Benita; Matschiavelli, Nicole; Hübner, René; Matschi, Susanne; Hoehenwarter, Wolfgang; Sachs, Susanne

doi:10.1016/j.scitotenv.2022.153700

Cited by 15 publications

(10 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESCRT complexes), and in the secretory pathway were significantly deregulated ( Figure 8C; Figure S6 ). The involvement of endocytosis in U uptake has been recently proposed in tobacco cells (John et al, 2022). Here, a hypothetical scenario would be that uranyl ions, U target proteins or other proteins involved in U tolerance are internalized into vesicles to prevent any toxicity before being released towards the apoplast or stored/degraded in the vacuole.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

Przybyla-Toscano,

Chetouhi,

Balliau

et al. 2024

Preprint

View full text Add to dashboard Cite

Uranium (U) is a non-essential and toxic metal for plants, which have the ability to take up uranyl ions from the soil and preferentially accumulate them in the roots. We showed that the bulk of the radionuclide accumulates in the root insoluble proteome of Arabidopsis plants challenged with U. Therefore, to elucidate new molecular mechanisms related to U stress response and tolerance, we used label-free quantitative proteomics to analyze the dynamics of the root membrane- and cell wall-enriched proteome under U stress. Of the 2,802 proteins identified, 458 showed differential accumulation in response to U. Biological processes affected by U include response to stress, amino acid metabolism, and previously unexplored functions associated with membranes and the cell wall. Indeed, our analysis supports a dynamic and complex reorganization of the cell wall in response to U stress, including lignin and suberin synthesis, pectin modifications, polysaccharide hydrolysis, and Casparian strips formation. Water flux through aquaporins and vesicular trafficking were also significantly perturbed by U stress. Finally, the abundance of metal transporters and iron, calcium, and other metal-binding proteins was affected by U. These proteins may play a role in controlling the fate and toxicity of U in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

“…In addition, the calcium concentration in the nutrient medium modulates U responses in shoots (Mertens et al, 2022). Beside transporter-mediated uptake, an additional pathway implying endocytic uptake might also be important for U transport into plant cells (John et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

Przybyla-Toscano,

Chetouhi,

Balliau

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Cell cultures of Brassica napus, Daucus carota and Nicotiana tabacum have already been utilized to study the cytotoxicity and bioassociation of Cm(III), U(VI) and Eu(III) in biochemical, metabolic and spectroscopic aspects. 29,[31][32][33][34][35] Among a multitude of cell lines derived from higher plants, suspension cell cultures of Nicotiana tabacum L. cv. Bright Yellow-2 (BY-2) are widely used because of their high growth rate, high homogeneity and modest cultivation in the laboratory.…”

Section: Introductionmentioning

confidence: 99%

“…Bright Yellow-2 (BY-2) are widely used because of their high growth rate, high homogeneity and modest cultivation in the laboratory. 36 While BY-2 cells have been used to study the phytotoxicity of pharmaceuticals, the stress response in the presence of heavy metals, their interaction with actinides, or the effects of various types of nanoparticles on cells, 33,34,[37][38][39][40][41][42] reports on the effect of Ln on cell metabolism and integrity as well as on metal uptake and distribution remain scarce.…”

Section: Introductionmentioning

confidence: 99%

How tobacco (Nicotiana tabacum) BY-2 cells cope with Eu(iii) – a microspectroscopic study

Klotzsche,

Vogel,

Sachs

et al. 2023

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…In recent years, our knowledge of U toxicity and the molecular mechanisms used by land plants to respond to U stress has increased significantly (Chen 2021). Very recent studies shed light on the uptake of U through calcium channels in root cells (Sarthou 2022) or by endocytosis in cultured plant cells (John 2022), and identified uranium-binding proteins in Arabidopsis thaliana (Vallet 2023). The fate of U is less well understood in microalgae than in land plants.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a uranium-tolerant green microalga of the genusCoelastrellawith high potential for the remediation of metal-polluted waters

Beaulier,

Dannay,

Devime

et al. 2023

Preprint

View full text Add to dashboard Cite

Uranium (U) pollution of terrestrial and aquatic ecosystems poses a significant threat to the environment and human health because this radionuclide is chemotoxic. Characterization of organisms that tolerate and accumulate U is critical to decipher the mechanisms evolved to cope with the radionuclide and to propose new effective strategies for bioremediation of U-contaminated environments. Here, we isolated a unicellular green microalga of the genus Coelastrella from U-contaminated wastewater. We showed that Coelastrella sp. PCV is much more tolerant to U than Chlamydomonas reinhardtii and Chlorella vulgaris. Coelastrella is able to accumulate U very rapidly, then gradually release it into the medium, behaving as an excluder to limit the toxic effects of U. The ability of Coelastrella to accumulate U is remarkably high, with up to 600 mg U sorbed per g dry biomass. Coelastrella is able to grow and maintain high photosynthesis in natural metal-contaminated waters from a wetland near a reclaimed U mine. Over a single one-week growth cycle, Coelastrella is able to capture 25-55% of U from contaminated waters and demonstrates lipid droplet accumulation. Coelastrella sp. PCV is a very promising microalga for the remediation of polluted waters with valorization of algal biomass that accumulates lipids.

show abstract

Endocytosis is a significant contributor to uranium(VI) uptake in tobacco (Nicotiana tabacum) BY-2 cells in phosphate-deficient culture

Cited by 15 publications

References 75 publications

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress

How tobacco (Nicotiana tabacum) BY-2 cells cope with Eu(iii) – a microspectroscopic study

Characterization of a uranium-tolerant green microalga of the genusCoelastrellawith high potential for the remediation of metal-polluted waters

Contact Info

Product

Resources

About