Endocytosis in freshwater amebas.

Chapman-Andresen, C

doi:10.1152/physrev.1977.57.3.371

Cited by 29 publications

(12 citation statements)

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“…Treatment of cells with 0 .1% Alcian blue caused the cells to stop moving (19,20) and to form pinocytotic channels (5,16) (Figs. 3 c and 9, black arrow).…”

Section: Redistribution Of Actin After Experimental Manipulationmentioning

confidence: 99%

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

Taylor¹,

Wang²,

Heiple³

1980

The Journal of Cell Biology

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The technique of molecular cytochemistry has been used to follow the distribution of fluorescently labeled actin in living Chaos carolinensis and Amoeba proteus during ameboid movement and various cellular processes. The distribution of 5-iodoacetamidofluorescein-labeled actin was compared with that of Lissamine rhodamine B sulfonyl chloride-labeled ovalbumin microinjected into the same cell and recorded with an image intensification microscope system. Actively motile cells demonstrated a rather uniform distribution of actin throughout most of the cytoplasm, except in the tail ectoplasm and in plasma gel sheets, where distinct actin structures were observed. In addition, actin-containing structures were induced in the cortex during wound healing, concanavalin A capping, pinocytosis, and contractions elicited by phalloidin injections. The formation of distinct fluorescent actin structures has been correlated with contractile activities.

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“…Treatment of cells with 0 .1% Alcian blue caused the cells to stop moving (19,20) and to form pinocytotic channels (5,16) (Figs. 3 c and 9, black arrow).…”

Section: Redistribution Of Actin After Experimental Manipulationmentioning

confidence: 99%

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

Taylor¹,

Wang²,

Heiple³

1980

The Journal of Cell Biology

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“…A great deal of quantitative information is available on both the physiology and morphology of free-living amebac (19) and extensive analysis of contractility and of endocytic mechanisms in these cells has been carried out over the last fifty years (7,30). The exceptionally large size of C. carolinensis…”

mentioning

confidence: 99%

pH changes in pinosomes and phagosomes in the ameba, Chaos carolinensis.

Heiple¹,

Taylor²

1982

The Journal of Cell Biology

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Changes in pH are measured in pinosomes and phagosomes of single specimens of the giant, free-living ameba, Chaos carolinensis. Measurements of pH are made microfluorometrically, as previously described (Heiple and Taylor. 1980. J. Cell Biol. 86:885-890.) by quantitation of fluorescence intensity ratios (Ex489nm,/Ex452nm, Ems2o-s6onm from ingested fluorescein thiocarbamyl (FTC)-ovalbumin. After I h of pinocytosis (induced in acid solution), FTCovalbumin is found in predominantly small (--<5 #m in diameter), acidic (pH -< 5.0-6.2) vesicles of various shape and density. As the length of ingestion time increases (up to 24 h), the probe is also found in vesicles of increasing size (up to 100 #m in diameter), increasing pH (up to pH -8.0), and decreasing density. Co-localization of fluorescein and rhodamine fluorescence, after a pulse-chase with fluorescein-and rhodamine-labeled ovalbumin, suggests vesicle growth, in part, by fusion. The pH in a single phagosome is followed after ingestion of ciliates in neutral solutions of FTC-ovalbumin. A dramatic acidification (A pH > -2.0) begins within 5 min of phagosome formation and appears to be complete in -20 min. Phagosomal pH then slowly recovers to more neutral values over the next 2 h. pH changes observed in more mature populations of pinosomes within a single cell may reflect those occurring within a single phagosome. Phagosomal and pinosomal pH changes may be required for lysosomal fusion and may be involved in regulation of lysosomal enzyme activity.Little is known about the regulation of intracellular vesicle movements after internalization of macromoleculcs or particles by endocytosis (30). Quantitative information about the normal sequence of ionic changes within endosomes is needed to understand the regulation of endosomal events such as endosome-lysosome fusion and the degradation or protection of internalized substances. Until recently, no satisfactory quantitative technique existed for the continuous measurement of pH within specific subcellular compartments of a single, moving eucaryotic cell. The novel microfluorometric technique we designed and applied to cytoplasmic pH measurements in single motile amebae (11) is here extended to a study of pH changes in pinosomes and phagosomes of these amebac.The free-living, fresh-water amebae, including Chaos carolinensis, are ideal cells for the study of a variety of fundamental processes. A great deal of quantitative information is available on both the physiology and morphology of free-living amebac (19) and extensive analysis of contractility and of endocytic mechanisms in these cells has been carried out over the last fifty years (7,30). The exceptionally large size of C. carolinensis

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“…Finally, as an echo from the early days of pinocytosis and its sequels at Carlsberg Laboratorium, I was interested to notice, among the first references in a paper from 1983 "Phagocytepathogenic microbe interactions" (59), two reviews on pinocytosis, the first by HOLTER from 1959 (32), the second by a former co-worker (14).…”

Section: Resultsmentioning

confidence: 99%

“…However, some of these studies continue in Copenhagen, at the University's Institute of Cell Biology and Anatomy, where the first observations on vacuole formation in Tetrahymena (23) have led to a series of papers by NILSSON (55,56,57,61), on various aspects of uptake, combined with physiological and fine structural studies. Fresh water amoebae are still cultured at the same Institute, at which studies on pinocytosis still proceed (13,14). These studies at the University of Copenhagen have been made possible by the generous support of the Carlsberg Foundation.…”

Section: Studies On Pinocytosis Atmentioning

confidence: 99%

The early days of pinocytosis

Chapman-Andresen

1984

Carlsberg Res. Commun.

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Endocytosis in freshwater amebas.

Cited by 29 publications

References 0 publications

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

pH changes in pinosomes and phagosomes in the ameba, Chaos carolinensis.

The early days of pinocytosis

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