Endocytosis and Intracellular Trafficking of Quantum Dot–Ligand Bioconjugates

Iversen, Tore Geir; Frerker, Nadine; Sandvig, Kirsten

doi:10.1002/9780470875780.ch4

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…Therefore, much research effort is currently devoted to determining what parameters affect the internalization pathway to avoid lysosomal degradation and/or escaping endosomal vesicles to the cytosol. Particles with varying degrees of sizes and charges have been investigated to determine what conditions favor internalization methods that are not directed toward lysosomal pathways (102,103), but there is no consensus on the optimal conditions for such nondegradative endosomal pathways (99). One approach for lysosomal escape uses the proton sponge effect, in which particles are designed to absorb protons upon the acidification of the vesicle, disrupting the membrane by increasing osmotic pressure (104); another approach uses light-sensitive molecules that can be activated to generate reactive oxygen species, which degrade the vesicle components (105).…”

Section: Intracellular Trapping and Escapementioning

confidence: 99%

Physical Chemistry of Nanomedicine: Understanding the Complex Behaviors of Nanoparticles in Vivo

Lane

Qian

Smith

et al. 2015

Annu. Rev. Phys. Chem.

157

174

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Nanomedicine is an interdisciplinary field of research at the interface of science, engineering, and medicine, with broad clinical applications ranging from molecular imaging to medical diagnostics, targeted therapy, and image-guided surgery. Despite major advances during the past 20 years, there are still major fundamental and technical barriers that need to be understood and overcome. In particular, the complex behaviors of nanoparticles under physiological conditions are poorly understood, and detailed kinetic and thermodynamic principles are still not available to guide the rational design and development of nanoparticle agents. Here we discuss the interactions of nanoparticles with proteins, cells, tissues, and organs from a quantitative physical chemistry point of view. We also discuss insights and strategies on how to minimize nonspecific protein binding, how to design multistage and activatable nanostructures for improved drug delivery, and how to use the enhanced permeability and retention effect to deliver imaging agents for image-guided cancer surgery.

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Section: Intracellular Trapping and Escapementioning

confidence: 99%

Physical Chemistry of Nanomedicine: Understanding the Complex Behaviors of Nanoparticles in Vivo

Lane

Qian

Smith

et al. 2015

Annu. Rev. Phys. Chem.

157

174

View full text Add to dashboard Cite

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The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

Frőhlich¹

2012

IJN

1,991

1,416

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Many types of nanoparticles (NPs) are tested for use in medical products, particularly in imaging and gene and drug delivery. For these applications, cellular uptake is usually a prerequisite and is governed in addition to size by surface characteristics such as hydrophobicity and charge. Although positive charge appears to improve the efficacy of imaging, gene transfer, and drug delivery, a higher cytotoxicity of such constructs has been reported. This review summarizes findings on the role of surface charge on cytotoxicity in general, action on specific cellular targets, modes of toxic action, cellular uptake, and intracellular localization of NPs. Effects of serum and intercell type differences are addressed. Cationic NPs cause more pronounced disruption of plasma-membrane integrity, stronger mitochondrial and lysosomal damage, and a higher number of autophagosomes than anionic NPs. In general, nonphagocytic cells ingest cationic NPs to a higher extent, but charge density and hydrophobicity are equally important; phagocytic cells preferentially take up anionic NPs. Cells do not use different uptake routes for cationic and anionic NPs, but high uptake rates are usually linked to greater biological effects. The different uptake preferences of phagocytic and nonphagocytic cells for cationic and anionic NPs may influence the efficacy and selectivity of NPs for drug delivery and imaging.

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Endocytosis and Intracellular Trafficking of Quantum Dot–Ligand Bioconjugates

Cited by 2 publications

References 63 publications

Physical Chemistry of Nanomedicine: Understanding the Complex Behaviors of Nanoparticles in Vivo

Physical Chemistry of Nanomedicine: Understanding the Complex Behaviors of Nanoparticles in Vivo

The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles

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