Endocrine profile in serum and follicular fluid differs after ovarian stimulation with HP-hMG or recombinant FSH in IVF patients

Smitz, Johan; Andersen, A. Nyboe; Devroey, Paul; Arce, Joan‐Carles

doi:10.1093/humrep/del445

Cited by 173 publications

(166 citation statements)

References 57 publications

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“…In fact, different stimulation regimens cause several differences in the FF levels of hormones and peptides. The addition of LH/hCGcontaining preparations, such as hMG, from the beginning of stimulation induces higher production of E 2 and lower production of P 4 respect to stimulation with FSH alone [52]. These data support the notion that a low dosage of LH from the beginning of stimulation might be necessary to support the production of androgens by TCs and as a consequence the production of E 2 by GCs.…”

Section: Introductionsupporting

confidence: 70%

“…In fact, it has been shown that patients stimulated with hMG, presenting LH activity, show lower levels of P 4 and higher levels of E 2 compared to those stimulated with r-FSH alone [52] However, this apparent discrepancy can be explained by the fact that, in the case of hMG stimulation, when growing follicles are exposed to the LH activity present in the hMG at the beginning of stimulation, when only TCs respond to LH, there is an increase in androgen production that, in turn, stimulates estrogen synthesis. On the contrary, when the Fig.…”

Section: Discussionmentioning

confidence: 99%

“…These data support the notion that a low dosage of LH from the beginning of stimulation might be necessary to support the production of androgens by TCs and as a consequence the production of E 2 by GCs. Moreover, differences have also been found in the levels of VEGF, inhibin A and B [52] and TGF-β [28]. It is noteworthy that AMH and TGF-β are differentially regulated in the preovulatory follicle.…”

Section: Introductionmentioning

confidence: 99%

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Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Barberi

Ermini

Morelli

et al. 2012

J Assist Reprod Genet

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Purpose Down-regulation with gonadodropin-releasing agonist (GnRH-a) protocol during IVF stimulation leads to a severe endogenous LH suppression, which may affect the follicular development. The aim of the study was to evaluate the effects of recombinant LH (r-LH) administration, during late follicular development stages, in recombinant FSH (r-FSH) stimulated cycles on follicular fluid (FF) parameters and on cumulus cell quality. Methods Twenty patients undergoing IVF were stimulated in a long GnRH agonist protocol with r-FSH alone or with r-LH supplementation when the leading follicle reached diameter of 14 mm. FF was collected at the time of oocyte retrieval from 32 follicles ≥ 18 mm. Serum FSH, LH, estradiol (E 2 ), and progesterone (P 4 ) were evaluated on the day of hCG administration. Intra-follicular E 2 , P 4 , AMH and TGF-β were assayed. Total RNA from 18 individual cumuli was isolated for gene expression analyses. Results R-LH increased FF P 4 levels. FF TGF-β levels and PTGS2 and HAS2 expression in cumulus cells (CCs) positively correlated with increased P 4 levels observed in FFs, while a negative correlation was found between P 4 and AMH levels. Conclusions FF positive correlation between P 4 and TGF-β levels and CC expression of PTGS2 and HAS2 suggest an association with a better follicle quality. In addition, our data suggest that late follicular phase r-LH supplementation leads to a more advanced stage of follicular maturation.

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Section: Introductionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Barberi

Ermini

Morelli

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

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“…Using LC-MS/MS methods (Kushnir et al, , 2006a(Kushnir et al, ,b, 2008, concentrations of endogenous steroids and the patterns of distribution of the steroids in FF were determined in healthy women and in women after ovarian stimulation for IVF treatment (Kushnir et al, 2009). The difference of this study from earlier published studies (Dehennin, 1990;De Sutter et al, 1991;Andersen, 1993;Smitz et al, 2007) was in the use of more sensitive and specific methods, which allowed simultaneous measurement of a large number of bioactive steroids and the intermediates of their biosynthesis in samples from individual follicles. The majority of earlier studies of ovarian steroidogenesis used IAs for determining concentrations, and each study focused on the effect of one or few of the steroids on the follicular development.…”

Section: Steroids In Ovarian Follicles Of Healthy Womenmentioning

confidence: 94%

Liquid chromatography-tandem mass spectrometry applications in endocrinology

Kushnir

Rockwood

Bergquist

2009

Mass Spectrom. Rev.

138

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Liquid chromatography-tandem mass spectrometry (LC-MS/ MS) has been recognized as a primary methodology for the accurate analysis of endogenous steroid hormones in biological samples. This review focuses on the use of LC-MS/MS in clinical laboratories to assist with the diagnosis of diverse groups of endocrine and metabolic diseases. Described analytical methods use on-line and off-line sample preparation and analytical derivatization to enhance analytical sensitivity, specificity, and clinical utility. Advantages of LC-MS/MS as an analytical technique include high specificity, possibility to simultaneously measure multiple analytes, and the ability to assess the specificity of the analysis in every sample. All described analytical methods were extensively validated, utilized in routine diagnostic practice, and were applied in a number of clinical and epidemiological studies, including a study of the steroidogenesis in ovarian follicles. #

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“…The COH protocol involved downregulation with a GnRH agonist followed by gonadotrophin stimulation with either HP-hMG or rFSH, and ultimately, oocyte maturation was flared up with hCG treatment. FF collected at the time of oocyte retrieval showed marked difference between the HP-hMG and rFSH groups in the concentrations of FSH, LH, hCG, E 2 , P 4 , androstenedione (A 4 ), testosterone (T), inhibin A and VEGF as well as in the ratios of E 2 /P 4 , E 2 /A 4 , E 2 /T and inhibin A/inhibin B [60], whereas leptin levels depended strongly on the serum levels [61], while TGFβ-1 and IGFBP-1 were unaffected [61]. A comparison between hMG, clomiphene citrate and natural cycles also revealed that the hMG cycle resulted in lower VEGF concentrations in FF than clomiphene citrate or natural cycles, but more importantly, VEGF concentrations in FF were negatively correlated with the number of follicles, irrespective of the ovulation induction protocol [62].…”

Section: Hormonal Stimulationmentioning

confidence: 99%

Factors Influencing the Biochemical Markers for Predicting Mammalian Oocyte Quality

Ola

Sun

2012

Journal of Reproduction and Development

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Abstract. The need for accurate selection of the best oocytes for in vitro fertilization protocols and thus, production of embryos has driven the search for oocyte quality markers from morphological criteria to biochemical parameters. Current studies are focused on the biochemical constituents of the follicular fluid and gene expression profiling of the cumulus cells. These parameters are, however, affected by factors that must be considered before making a judgment of the oocyte's quality. These includes factors such as the type of hormonal stimulation protocol, age of oocyte donor and heat stress on the donor, all of which have been reported to influence the concentrations of many hormones, apolipoproteins, metabolites, fatty acids and growth factors in the follicular fluid and the expression of several genes in the cumulus cells. Another important point to note is species variation in the response to these extraneous influences, which thus calls for species targeted investigations. As reports are still scanty and investigations assumed to be very keen, we employed this review paper to bring attention of researchers and clinicians to those factors that may come to bear on the outcome of their investigations on oocyte and embryo quality. Key words: Cumulus cells, Follicular fluid, Mammalian oocytes, Quality prediction (J. Reprod. Dev. 58: [385][386][387][388][389][390][391][392] 2012) I t is well known that only part of the in vivo ovulated oocytes and in vitro matured oocytes are capable of developing to viable embryos after fertilization. This led to the search for a reliable indicator of oocyte quality. Thus, one of the important puzzles for reproductive biologists today is the search for a reliable noninvasive marker of the quality of oocytes for both human and animal assisted reproduction.Initial attempts at evaluating the quality of oocytes meant for in vitro fertilization (IVF) focused on the physical/morphological characteristics of the Cumulus-oocyte-complexes (COCs) [1,2]. These criteria are both noninvasive and rapid and are still generally in use today. However, recent reports on the inadequacies of a simple morphological appearance as a measure of quality suggest that morphological markers cannot be completely relied upon [3,4]. This has led to intensification of the search for alternative or complementary criteria with focus on the follicular fluid (FF) and the cumulus cells (CCs) around the oocyte.The preovulatory oocyte communicates intimately and directly with the surrounding cumulus granulosa cells through specialized transzonal cytoplasmic projections [5] and with the more distant mural granulosa cells through the follicular fluid [6]. Many biochemical substances found in these close associates of the oocyte have been studied and correlated with the developmental competence of the oocyte. Very recent attempts are even looking beyond a single substance to the totality of the biochemical constituents of the follicular fluid. This area of research is referred to as metabolomics [7]. Revelli e...

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Endocrine profile in serum and follicular fluid differs after ovarian stimulation with HP-hMG or recombinant FSH in IVF patients

Abstract: Major differences in serum and follicular fluid endocrine profile exist after stimulation with HP-hMG or rFSH. Exogenous LH activity induces a differential endocrine environment influencing oocyte quantity and quality, which may be of relevance for clinical outcome.

Cited by 173 publications

References 57 publications

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Follicular fluid hormonal profile and cumulus cell gene expression in controlled ovarian hyperstimulation with recombinant FSH: effects of recombinant LH administration

Liquid chromatography-tandem mass spectrometry applications in endocrinology

Factors Influencing the Biochemical Markers for Predicting Mammalian Oocyte Quality

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