Endocrine Pancreatic Cells fromXenopus laevis:Light and Electron Microscopic Studies

Lozano, Marı́a Teresa; Hernández, M. P. García; Agulleiro, B.

doi:10.1006/gcen.1998.7247

Cited by 8 publications

(12 citation statements)

References 33 publications

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“…4,9,8,13 In the present study, cells stained for glucagon were found as single cells or clusters in the pancreas of the Korean golden frog (Figure 2A,B), similar to the insulin positive cells and other Salientia. 4,9,8,13 In addition, glucagons-containing cells showed the second highest abundance in the present study, 54.30±8.77/1000 cells and 26.02±1.71% of all IR cel1s was positive for glucagon (Table 2). These results are similar to those of European common frog4 and the red-bellied frog.…”

Section: Resultssupporting

confidence: 78%

“…It is well recognized that insulin positive cells are present throughout the Salientia pancreas either in single cells or as clusters based on the previous studied of the European common frog, 4,11 African clawed toad 8,12,13 and red-bellied frog. 9 Our results in the present study concurred with those of the above studies in that the cells stained for insulin were found to be localized in the pancreas of the Korean golden frog as single cells or clusters (Figure 1A,B).…”

Section: Resultsmentioning

confidence: 99%

“…In the Salientian pancreas, cells stained for somatostatin were detected in the European common frog, 4 the African clawed toad, 6–8,13 the green frog, 5 and the red-bellied frog. 9 They were present as single cells or clusters in the pancreas of these species, including, in the present study, the Korean golden frog, and in the case of cluster, they occupied marginal regions (Figure 3A−C).…”

Section: Resultsmentioning

confidence: 99%

“…12,13 However, in the red-bellied frog, 9 they were randomly dispersed in the pancreatic parenchyma only as single cells, between exocrine acinar cells. In the present study, cells stained for PP were also found as single cells or clusters in the pancreas of the Korean golden frog.…”

Section: Resultsmentioning

confidence: 99%

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An immunohistochemical study of the pancreatic endocrine cells of the Korean golden frog, Rana plancyi chosenica

Lee

Chang

2012

Eur J Histochem

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The regional distribution and quantitative frequency of pancreatic endocrine cells were demonstrated in the Korean golden frog (Rana plancyi chosenica Okada), which is known as a Korean endemic species, for the first time by immunohistochemical methods using specific mammalian antisera to insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). In the pancreas of the Korean golden frog, all four endocrine cell types were demonstrated. Insulin- and glucagon-positive cells were located in the pancreas as single cells or islet-like clusters, respectively. Somatostatin-containing cells were also dispersed in the pancreas as single cells or clusters but in the case of clusters, they are exclusively situated in the marginal regions of insulin- or glucagon-positive cell clusters. PP-containing cells were also distributed as single cells or clusters. Clusters consisted of PP-positive cells are distributed as a core type and a marginally distributed type. Overall, there were 40.84±3.81% insulin-, 26.02±1.71% glucagon-, 7.63±2.09% somatostatin- and 25.51±3.26% PP-IR cells.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

An immunohistochemical study of the pancreatic endocrine cells of the Korean golden frog, Rana plancyi chosenica

Lee

Chang

2012

Eur J Histochem

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“…3A). These cells resembled the cells of exocrine pancreas in vivo ( Lozano et al 1999 ). In addition to the acinus‐like structures, two types of cells with differing secretory granules were found around the acinus‐like structures.…”

Section: Resultsmentioning

confidence: 99%

In vitro pancreas formation from Xenopus ectoderm treated with activin and retinoic acid

et al. 2000

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In the present study, isolated presumptive ectoderm from Xenopus blastula was treated with activin and retinoic acid to induce differentiation into pancreas. The presumptive ectoderm region of the blastula consists of undifferentiated cells and is fated to become epidermis and neural tissue in normal development. When the region is isolated and cultured in vitro, it develops into atypical epidermis. Isolated presumptive ectoderm was treated with activin and retinoic acid. The ectoderm frequently differentiated into pancreas-like structures accompanied by an intestinal epithelium-like structure. Sections of the explants viewed using light and electron microscopy showed some cells clustered and forming an acinus-like structure, including secretory granules. The pancreas-specific molecular markers insulin and XIHbox8 were also expressed in the treated explants. The pancreatic hormones, insulin and glucagon, were detected in the explants using immunohistochemistry. Therefore, sequential treatment with activin and retinoic acid can induce presumptive ectoderm to differentiate into a morphological and functional pancreas in vitro. When ectoderm was immediately treated with retinoic acid after treatment with activin, well-differentiated pronephric tubules were seen in a few of the differentiated pancreases. Treatment with retinoic acid 3-5 h after activin treatment induced frequent pancreatic differentiation. When the time lag was longer than 15h, the explants developed into axial mesoderm and pharynx. The present study provides an effective system for analyzing pancreas differentiation in vertebrate development.

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Ultrastructure of endocrine pancreatic granules during pancreatic differentiation in the grass snake, Natrix natrix L. (Lepidosauria, Serpentes)

Kowalska

Rupik

2017

Journal of Morphology

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We used transmission electron microscopy to study the pancreatic main endocrine cell types in the embryos of the grass snake Natrix natrix L. with focus on the morphology of their secretory granules. The embryonic endocrine part of the pancreas in the grass snake contains four main types of cells (A, B, D, and PP), which is similar to other vertebrates. The B granules contained a moderately electron-dense crystalline-like core that was polygonal in shape and an electron-dense outer zone. The A granules had a spherical electron-dense eccentrically located core and a moderately electron-dense outer zone. The D granules were filled with a moderately electron-dense non-homogeneous content. The PP granules had a spherical electron-dense core with an electron translucent outer zone. Within the main types of granules (A, B, D, PP), different morphological subtypes were recognized that indicated their maturity, which may be related to the different content of these granules during the process of maturation. The sequence of pancreatic endocrine cell differentiation in grass snake embryos differs from that in many vertebrates. In the grass snake embryos, the B and D cells differentiated earlier than A and PP cells. The different sequence of endocrine cell differentiation in snakes and other vertebrates has been related to phylogenetic position and nutrition during early developmental stages.

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Endocrine Pancreatic Cells fromXenopus laevis:Light and Electron Microscopic Studies

Cited by 8 publications

References 33 publications

An immunohistochemical study of the pancreatic endocrine cells of the Korean golden frog, Rana plancyi chosenica

An immunohistochemical study of the pancreatic endocrine cells of the Korean golden frog, Rana plancyi chosenica

In vitro pancreas formation from Xenopus ectoderm treated with activin and retinoic acid

Ultrastructure of endocrine pancreatic granules during pancreatic differentiation in the grass snake, Natrix natrix L. (Lepidosauria, Serpentes)

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