Endocrine effects of environmental pollution on Xenopus laevis and Rana temporaria

Bögi, Christian; Schwaiger, Jens; Ferling, Hermann; Mallow, U.; Steineck, Cornelia; Sinowatz, Fred; Kalbfus, W.; Negele, R. D.; Lutz, Ilka; Kloas, Werner

doi:10.1016/s0013-9351(03)00082-3

Cited by 61 publications

(30 citation statements)

References 16 publications

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“…The percentage of intersex individuals at metamorphosis in this study is, however, higher than reported elsewhere. Bögi et al (2003) found that the sex ratio for control R. temporaria tadpoles was 56% females, 28% males and 16% intersex. However, the authors had kept the frogs a few weeks after metamorphosis before sampling and histological analysis.…”

Section: Gonadal Differentiationmentioning

confidence: 91%

Effects of prochloraz and ethinylestradiol on sexual development in Rana temporaria

Brande‐Lavridsen

Christensen‐Dalsgaard

Korsgaard

2008

J. Exp. Zool.

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A wide range of environmental xenobiotics that mimic hormones (endocrine-disrupting chemicals) may cause alterations in sexual development or reproductive function in aquatic organisms such as amphibians when exposed during early sensitive stages. We exposed tadpoles of the Common frog, Rana temporaria, from hatch to metamorphosis, to two different endocrine disruptors, the synthetic estrogen 17 alpha-ethinylestradiol and the fungicide prochloraz. The object of the study was to assess the effects of these two compounds on the sexual development of the tadpoles by investigating sex ratio, gonadal development, sex steroid concentrations and vitellogenin induction. Histology revealed that a large percentage of all groups were juvenile hermaphrodites at metamorphosis. Tadpoles exposed to 115 and 251 microg/L prochloraz showed a significant increased proportion of males. However, the testosterone concentrations were depressed in those groups. Ethinylestradiol in concentrations of 77 and 159 ng/L EE(2) increased whole-body calcium levels in a dose-dependent manner indicating induction of the egg yolk protein vitellogenin, verified also by gel electrophoresis. The study shows that ethinylestradiol may induce vitellogenesis and prochloraz may affect the sexual development in Common frogs.

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Section: Gonadal Differentiationmentioning

confidence: 91%

Effects of prochloraz and ethinylestradiol on sexual development in Rana temporaria

Brande‐Lavridsen

Christensen‐Dalsgaard

Korsgaard

2008

J. Exp. Zool.

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“…Exposure of R. temporaria hepatocytes to both estrone and E 2 increased vtg mRNA synthesis in a dose-dependent manner 9 . However, exogenous xenoestrogens and estrogen-mimic-containing sewage increased vtg mRNA expression levels in immature female X. laevis 21 . These different responses of vtg gene expression after E 2 injections are likely to be due to the different developmental stages of the animals assayed (immature or adult), the different experimental procedures used (in vitro or in vivo assays), or the differences among frog species.…”

Section: Discussionmentioning

confidence: 89%

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Ratanasaeng¹,

Chanchao²,

Pariyanonth³

et al. 2008

ScienceAsia

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ABSTRACT:We aimed to determine the vitellogenin gene expression in response to 17β-estradiol (E2) treatment in livers of immature female Chinese edible frogs (Hoplobatrachus rugulosus). Frogs reared during rainy or dry seasons were intramuscularly injected daily with E2 at doses of 0, 50, 500, or 5000 µg/kg body weight for five days. During the rainy season, treatment with E2 within the dose range studied significantly decreased the vitellogenin gene transcript levels compared to those of the control. In contrast, during the dry season, treatment with E2 at all doses tended to increase the vitellogenin gene transcript levels. The endogenous plasma E2 levels in frogs reared during the rainy season were higher than those reared during the dry season. There was no significant difference in liver-somatic index or gonadosomatic index among the treated frogs during the rainy season. However, during the dry season, the 500 µg/kg E2 treatment significantly increased the frog liver-somatic index. The hepatocytes from the dry season frogs appeared smaller and flatter. The histology of ovaries from rainy season frogs showed that the oocytes were bigger and of several stages. We therefore concluded that the injection of E2 into immature female H. rugulosus may stimulate vitellogenesis if administered during the dry season, when the endogenous E2 is lower. This knowledge could be used as a basis for inducing precocious maturation or mating of H. rugulosus outside the breeding season.

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“…Other laboratory studies have found that the concentrations of NP and OP were 2.80 and 0.28 mg/L, respectively, in the municipal effluent from a WWTP in the United Kingdom [27] and 23 to 250 ng/L and 2 to 11 ng/L, respectively, in the effluent of a regional sewage treatment plant in South Bavaria [7]. In China, the concentrations of OP, NP, and BPA were 1,008.4b to 2,473.5 ng/L, 34.8 to 67.7 ng/L, and 36.2 to 56.8 ng/L in the effluent, respectively [28].…”

Section: Discussionmentioning

confidence: 91%

“…Feminization was also observed in the freshwater bream (Abramis brama) and the estuarine flounder (Platichthys flesus) in an industrial harbor in The Netherlands that receives effluent from WWTPs and in the hornyhead turbot (Pleuronichthys verticalis) in South California Bay, USA, which is located near a municipal wastewater outfall [5,6]. Additionally, Xenopus laevis and Rana temporaria exposed to the effluent from WWTPs in Bavaria, Germany, were found to have an altered sex ratio [7]. Finally, male rare minnows (Gobiocypris rarus) exposed to influent and effluent from WWTPs in China were found to have increased levels of Vtg (an egg yolk protein usually associated with adult females) [8].…”

Section: Introductionmentioning

confidence: 90%

Assessment of estrogenic activity conducted by combining bioassay and chemical analyses of the effluent from wastewater treatment plants in Nanjing, China

Zhang

Chao

2010

Enviro Toxic and Chemistry

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The estrogenic activity of the effluent from three municipal wastewater treatment plants (WWTPs) in Nanjing, China, was investigated. The water samples were enriched by solid-phase extraction and then eluted with different polar solvents, which gave 11 fractionated extracts. Chemical analysis and a vitellogenin (Vtg) assay in goldfish (Carassias auratus) were then utilized to evaluate the estrogenic activities 7 d after a single injection of the effluent extracts of WWTPs and to identify causative agents that led to the induction of Vtg in male fish. The results reveal that Vtg induction occurred primarily in response to the 75 to 90% methanol extracts, and different concentrations of the natural estrogens estrone (E(1)) and 17beta-estradiol (E(2)) were detected in these extracts. As the Vtg induction increased, the plasma E(2) levels increased, and a correlation between Vtg and E(2) does exist. Furthermore, the gonadal somatic index (GSI) did not decrease significantly (p > 0.05) when the Vtg concentrations were elevated after 7 d. Different concentrations of estrogens were detected in the effluents, which demonstrated that the current treatment processes employed by the three WWTPs could not fully remove these compounds. As a result, the aquatic organisms in the receiving water (Yangtze River) were at a risk of feminization.

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Endocrine effects of environmental pollution on Xenopus laevis and Rana temporaria

Cited by 61 publications

References 16 publications

Effects of prochloraz and ethinylestradiol on sexual development in Rana temporaria

Effects of prochloraz and ethinylestradiol on sexual development in Rana temporaria

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Assessment of estrogenic activity conducted by combining bioassay and chemical analyses of the effluent from wastewater treatment plants in Nanjing, China

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