Endocrine disruptors and human reproductive failure: the in vitro effect of phthalates on human luteal cells

Romani, Federica; Tropea, Anna; Scarinci, Elisa; Federico, Alex; Russo, Cinzia Dello; Lisi, Lucia; Catino, Stefania; Lanzone, Antonio; Apa, Rosanna

doi:10.1016/j.fertnstert.2014.05.041

Cited by 46 publications

(32 citation statements)

References 41 publications

(63 reference statements)

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“…A few clinical studies also reported adverse effects of phthalates in human females, e.g., estrogenic and antiandrogenic toxicities of di-methyl phthalate, DEP, DnBP, DEHP and MEHP led to thelarche (premature breast development in girls aged less than 8 years) in Puerto Rico population (Colón et al, 2000), and thyroid dysfunction was observed in pregnant women exposed to DnBP (Huang et al, 2007). Phthalates (e.g., DEHP, DnBP and BBzP) also affected luteal steroidogenesis as well as the balance between luteotrophic and luteolytic factors in humans, suggesting the interference of phthalates in luteal function (Romani et al, 2014). In vitro luciferase reporter gene assay on MDA-kb2 cells (androgen sensitive cells) showed that DEHP and di-phenyl iso-phthalate induced luciferase activity in a GR-dependent manner, while bisphenols suppressed it (Kolšek et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Human ketosteroid receptors interact with hazardous phthalate plasticizers and their metabolites: an in silico study

Josh

Pradeep

Amma

et al. 2015

J of Applied Toxicology

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Phthalic acid esters or phthalates are ubiquitous environmental pollutants known for their adverse health effects in test animals and, of late, in humans. Thus, in this molecular docking study - using Glide (Schrödinger) - the molecular interactions of 31 ligands, including 12 diphthalates, their monophthalates and phthalic acid with selected human ketosteroid receptors, i.e., androgen (hAR), progesterone (hPR) and glucocorticoid (hGR) receptors were explored and their binding affinities were compared with that of corresponding natural steroids and a known endocrine disrupting xenobiotic, bisphenol A (BPA). Mostly, diphthalates and monophthalates showed the potential for antisteroidal activity by interacting with hAR, hPR and hGR. Of them, diphenyl phthalate showed the highest G score (-7.70 kcal mol(-1) ) with hAR, and the crucial amino acid (aa) residues in the ligand binding domain (LBD) of this receptor involved in the molecular interactions were Phe 764, Leu 704, Asn 705 and Thr 877. The mono-iso-decyl phthalate showed the highest G score (-8.36) with the hPR, and the crucial aa residues in the LBD interactions were Arg 766 Gln 725 and Phe 778. The mono-iso-decyl phthalate also showed more affinity (-8.44) towards hGR than the natural ligand, and the aa residues in the LBD interactions were Gln 570 and Met 604. In addition to these, some other phthalates established comparable interactions with certain aa residues located in the LBD of these receptors, which resulted in higher G scores. Contrastingly, BPA and some natural ligands tested in this study showed lower G scores with these receptors than certain phthalates reported herein, i.e., certain phthalates are more toxic than the proven toxic BPA. Copyright © 2015 John Wiley& Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Human ketosteroid receptors interact with hazardous phthalate plasticizers and their metabolites: an in silico study

Josh

Pradeep

Amma

et al. 2015

J of Applied Toxicology

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“…Endocrine disruptive effect of cobalt was shown by Romani et al (2014). HCG and cobalt chloride were shown to induce progesterone release, vascular endothelial growth factor release and mRNA expression in human luteal cells respectively.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Gautam¹,

Chaube²

2018

Turk. J. Fish. Aquat. Sci.

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The aim of the present study was to examine comparative effects of different concentrations of heavy metals such as cadmium (Cd), cobalt (Co), lead (Pb) and mercury (Hg) on catfish oocyte maturation and ovulation. Post-vitellogenic oocytes of the catfish H. fossilis were incubated with heavy metals at concentrations of 0.1, 1.0, 10 and 50ng/ml. Translucent follicles without germinal vesicle and opaque follicles with GV were scored after 4, 8, 16, and 24 hr of incubation. Percentage GV breakdown (GVBD) was calculated as an index of oocyte maturation. The results of present investigation suggested that the effect of heavy metals on oocyte maturation and ovulation was in the order of Pb > Hg >Cd>Co. Pb and Hg showed significant increases (P<0.05) in GVBD and ovulation at all concentrations during 4 and 8hr of the incubation. However, there was a dose -dependent decrease at 16 and 24hr. Cd and Co showed decline in GVBD and ovulation at 16 and 24 hr of the incubation. However, mercury and lead showed a stimulatory effect on ovulation at 16 and 24 hr compared to control (P<0.05). Thus, these results suggest that catfish oocytes are differentially influenced by heavy metals and are resistant to them at certain levels.

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“…Phthalates, especially DnBP, BBP and DEHP, have high values of log K ow (Table 2), characteristic of lipophilic compounds, which allows the accumulation of these compounds in the organic matter present in soils and sediments (Benjamin et al, 2015;Net et al, 2015;Gavala et al, 2003). The interfering nature of phthalates has been reported in a number of studies both in vivo and in vitro (Benjamin et al, 2015;Romani et al, 2014;Mankidy et al, 2013;Hashimoto et al, 2003;Picard et al, 2001;Körner et al, 1999). Phthalates became the subject of much concern once they are suspected to cause premature puberty (Tsai et al, 2016;Zhang et al, 2015;Fisher & Eugter, 2014;Colón et al, 2000).…”

Section: Phthalatesmentioning

confidence: 99%

Endocrine disruptors: strategies for determination and occurrence in marine environments

Cotrim¹,

Fahning²,

Rocha³

et al. 2016

RGCI

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Research examining the occurrence of endocrine disruptors (ED) in the marine environment has substantially increased. These contaminants have been observed in several environmental compartments and matrices, and they may cause severe adverse effects in humans and ecosystems. In this study more than 240 papers investigating the analytical developments regarding the analysis on ED in environmental matrices and the occurrence of these compounds were critically evaluated. Modern sample preparation procedures aiming the use of minimal sample manipulation, minimal amount of solvents and energy according to the Green Chemistry principles are widely used. The ED in marine environments occurs in trace concentrations and their quantification still represents a challenge. The effects of these contaminants in marine ecosystems are poorly understood. However, due to their large use, it is predicted that new analytical developments to deal with ED contamination will promote a large increase in the number of scientific publications in the near future. Regulations and mitigation measures for the presence of these contaminants in the environment are still scarce and need to be quickly implemented to reduce potential future adverse effects on ecosystem services of coastal environments. Cotrim et al. (2016) 300 RESUMO Pesquisas que examinam a ocorrência de interferentes endócrinos (IE) no ambiente marinho têm aumentado substancialmente. Estes contaminantes podem causar efeitos adversos em seres humanos e nos ecossistemas e têm sido observados em vários compartimentos e matrizes ambientais. Neste estudo, mais de 240 trabalhos que relataram o desenvolvimento analítico de IE em diversas matrizes e a ocorrência destes compostos no ambiente marinho foram criticamente avaliados. Procedimentos de preparo de amostra, visando a mínima manipulação, a mínima quantidade de solventes e energia de acordo com os princípios de Química Verde estão sendo amplamente utilizados. Os IE em ambientes marinhos ocorrem em concentrações traço e sua quantificação ainda representa um grande desafio. Como resultado, os efeitos desses contaminantes em ecossistemas marinhos ainda são mal compreendidos. No entanto, devido ao amplo uso destes compostos é previsto que novos desenvolvimentos analíticos para a determinação de IE irão promover um grande aumento no número de publicações científicas no futuro. Regulações e medidas mitigadoras para a ocorrência destes contaminantes ainda são bastante reduzida e precisam ser rapidamente implementadas para reduzir os futuros potenciais efeitos adversos nos serviços ecossistêmicos dos ecossistemas costeiros. Journal of Integrated Coastal Zone Management / Revista de Gestão Costeira Integrada, 16(3):299-326 (2016)

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Endocrine disruptors and human reproductive failure: the in vitro effect of phthalates on human luteal cells

Cited by 46 publications

References 41 publications

Human ketosteroid receptors interact with hazardous phthalate plasticizers and their metabolites: an in silico study

Human ketosteroid receptors interact with hazardous phthalate plasticizers and their metabolites: an in silico study

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Endocrine disruptors: strategies for determination and occurrence in marine environments

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