Endocrine disruptors and female fertility: Focus on (bovine) ovarian follicular physiology

Petro, Evi M.L.; Leroy, Jo; Cruchten, Steven Van; Covaci, Adrian; Jorssen, E.P.A.; Bols, P.E.J.

doi:10.1016/j.theriogenology.2012.06.011

Cited by 48 publications

(28 citation statements)

References 142 publications

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“…The current study offers the first thorough insight into the transcriptome analysis of primary bovine GCs during heat stress using DGE technology. The granulosa cells of the ovarian follicle support and nurture the oocyte and secrete estrogens which establish a functional bidirectional crosstalk with the oocyte through gap junctions and paracrine factors, and GCs are necessary for normal reproductive function (Langhout et al 1991;Petro et al 2012). Several studies have been published, demonstrating that the gene expression of GCs may differ in relation to the potential development of the embryo (McKenzie et al 2004;Wathlet et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian follicle development is a complex process. During follicle development, granulosa cells (GCs) replicate, secrete hormones, and provide a critical microenvironment for follicular growth (Petro et al 2012). Evidence indicates that multiple granulosa cell dysfunctions lead to disordered ovulatory and ovarian function (Tatone et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

Luo

et al. 2016

Cell Stress and Chaperones

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Summer heat stress (HS) is a major contributing factor in low fertility in lactating dairy cows in hot environments. Heat stress inhibits ovarian follicular development leading to diminished reproductive efficiency of dairy cows during summer. Ovarian follicle development is a complex process. During follicle development, granulosa cells (GCs) replicate, secrete hormones, and support the growth of the oocyte. To obtain an overview of the effects of heat stress on GCs, digital gene expression profiling was employed to screen and identify differentially expressed genes (DEGs; false discovery rate (FDR) ≤ 0.001, fold change ≥2) of cultured GCs during heat stress. A total of 1211 DEGs including 175 upregulated and 1036 downregulated ones were identified, of which DEGs can be classified into Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results suggested that heat stress triggers a dramatic and complex program of altered gene expression in GCs. We hypothesized that heat stress could induce the apoptosis and dysfunction of GCs. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the expression of steroidogenic genes (steroidogenic acute regulatory protein (Star), cytochrome P-450 (CYP11A1), CYP19A1, and steroidogenic factor 1 (SF-1)) and apoptosis-related genes (caspase-3, BCL-2, and BAX). Radio immunoassay (RIA) was used to analyze the level of 17β-estradiol (E 2 ) and progesterone (P 4 ). We also assessed the apoptosis of GCs by flow cytometry. Our data suggested that heat stress induced GC apoptosis through the BAX/BCL-2 pathway and reduced the steroidogenic gene messenger RNA (mRNA) expression and E 2 synthesis. These results suggest that the decreased function of GCs may cause ovarian dysfunction and offer an improved understanding of the molecular mechanism responsible for the low fertility in cattle in summer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

Luo

et al. 2016

Cell Stress and Chaperones

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“…However, there are always several issues that alter the balance of coastal dynamics, causing environmental impacts, largely motivated by conflicts of interest among uses such as recreation, port activity, and mining (Johnson-Restrepo et al, 2008;Gavio et al, 2010). Human impacts on nature, combined with natural processes, have triggered a largely ignored emergent phenomenon on organisms, such as changes in reproductive function derived from exposure to endocrine disruptors (Petro et al, 2012;Giusti and Joaquim-Justo, 2013;Halem et al, 2014). These studies point to the need for further research to determine the extent of which the human population may be affected.…”

Section: Introductionmentioning

confidence: 99%

Imposex in Stramonita haemastoma from coastal sites of Cartagena, Colombia

Sierra-Marquez

Rosa

et al. 2017

Braz. J. Biol.

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Imposex is the development of male sexual characteristics caused by the toxic effects of some chemicals that acts as an endocrinal disruptor. Antifouling paints contain these chemicals. Cartagena lacks studies to indicate the extent of imposex in its coastal waters. The aim of this study was to determine the prevalence of imposex in the gastropod Stramonita haemastoma in Cartagena, Colombia. Specimens were collected during 2013 from locations of high and low influence of port activity. Morphometric measurements and the frequency of the occurrence of imposex were registered. The comparison among morphometric variables showed statistically significant differences between the two sites studied. Furthermore, the females of the S. haemastoma species presented an imposex frequency of 93.1% in Birds' Island, Cartagena Bay, compared to 31.8% in La Bocana. The relative penis size index or RPLI (10.145 and 3.231) and vas deferens sequence index or VDSI (2.83 and 1.16), showed possible contamination by organotin compounds in both places.Keywords: imposex, bioaccumulation, boat paint, organotin compounds, penis development, toxicity. Palavras-chave: imposex, bioacumulação, pintura para barco, compostos organoestânicos, desenvolvimento do pênis, toxicidade. Imposex em

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“…EDC are found even in products considered inert, such as plastic (Hunt et al, 2003), and they are associated with some health problems in humans and animals, such as female and male subfertility (Annamalai and Namasivayam, 2015;Hond et al, 2015), certain types of cancers (Annamalai and Namasivayam, 2015), and neurological, developmental and reproductive disorders (Ness et al, 1993;Gore, 2008). These chemicals are claimed to be contributing to the fall in global fertility or be interacting with other causes for it, such as dietary factors (Petro et al, 2012). A worrying feature of EDC is that they usually have long half-lives, so they are persistent in environment (Bergeron et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Effects of 4-Nonylphenol on reproduction of exposed females during puberty

et al. 2016

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Abstract4-Nonylphenol (NP) is considered an endocrine disruptor due to its capacity to interact with endocrine system, mimicking estrogen activity. It is a ubiquitous pollutant since it has been found in many human tissue samples and its precursor identified in sewage, sediments, rivers and drinking water. Thus, this work aimed to evaluate reproductive parameters in female Balb/C mice treated with 50 mg/kg body weight of NP for 21 days, a period in which they reach sexual maturity. Body weight during treatment, day of vaginal opening, organs weight, fertility rate and proportion of different follicle types were some of these parameters. Additionally, for analysis of some effect in littermates, a group of animals underwent two attempts at pregnancy after treatment. NP had no effect on body weight during treatment, nor did it alter the day of vaginal opening and fertility rate of females. The proportion of preantral, antral and degenerated ovarian follicles was not modified by treatment with NP, and no morphological or pathological alterations were observed. The number of pups did not differ between treatments in any attempt at pregnancy. Also the weight of pups on birth and female rate in littermates was similar among experimental groups after both trials of pregnancy. It is suggested that the NP available or accumulated during the period of sexual maturation (21 days) did not reach threshold levels capable of producing significant alterations in the reproductive parameters analyzed.

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Endocrine disruptors and female fertility: Focus on (bovine) ovarian follicular physiology

Cited by 48 publications

References 142 publications

The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

Imposex in Stramonita haemastoma from coastal sites of Cartagena, Colombia

Effects of 4-Nonylphenol on reproduction of exposed females during puberty

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