Endocrine disruptor responses in African sharptooth catfish (Clarias gariepinus) exposed to di-(2-ethylhexyl)-phthalate

Adeogun, Aina O.; Ibor, Oju R.; Imiuwa, Maurice E.; Omogbemi, Emmanuel D.; Chukwuka, Azubuike V.; Omiwole, Roseline A.; Arukwe, Augustine

doi:10.1016/j.cbpc.2018.07.001

Cited by 21 publications

(12 citation statements)

References 77 publications

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“…In A. tenuis, exposure to irgarol for 7 d resulted in body color changes and reduced transcriptional expression of the HSP90 gene [29]. In a previous study, irgarol exposure induced expression changes of digestion related genes and chitinase genes [17]. Thus, VTG gene expression was affected by environmental chemical pollutants such as BPA, DEHP, and irgarol.…”

Section: Discussionmentioning

confidence: 85%

“…In addition, transcriptional responses of the VTG gene in the hepatopancreas were found to be significant under relatively low concentrations of DEHP for the different exposure times, whereas VTG was expressed in the ovaries under all concentrations of DEHP tested. Adeogun reported that DEHP exposure induced time-and concentration-dependent increases of VTG levels in C. gariepinus [17]. Long exposure to low doses of DEHP reduced oocyte maturation [46] and increased the levels of hepatic VTG transcripts in G. rarus [16].…”

Section: Discussionmentioning

confidence: 99%

“…Di-(2-ethylhexyl) phthalate (DEHP), a suspected EDC, is widely used as plasticizer and may induce reproductive and developmental toxicities in aquatic environments [15]. DEHP toxicity induced changes in the VTG levels in African sharptooth catfish (Clarias gariepinus) and the Chinese rare minnow (Gobiocypris rarus) [16,17]. DEHP levels were 0.47 to 12 µg L −1 in the China river estuary [18].…”

Section: Introductionmentioning

confidence: 99%

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Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

Park

Kim

et al. 2019

Applied Sciences

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Vitellogenesis is a pivotal reproductive process of the yolk formation in crustaceans. Vitellogenin (VTG) is the precursor of main yolk proteins and synthesized by endogenous estrogens. The intertidal mud crab (Macrophthalmus japonicus) inhabits sediment and is a good indicator for assessing polluted benthic environments. The purpose of this study was to identify potential responses of M. japonicus VTG under environmental stresses caused by chemical pollutants, such as 1, 10, and 30 µg L−1 concentrations in di(2-ethylhexyl) phthalate (DEHP), bisphenol A (BPA) and irgarol. We characterized the M. japonicus VTG gene and analyzed the transcriptional expression of VTG mRNA in M. japonicus exposed to various chemicals and exposure periods. A phylogenetic analysis revealed that the M. japonicus VTG clustered closely with Eriocheir sinensis (Chinese mitten crab) VTG, in contrast with another clade that included the VTG ortholog of other crabs. The basal level of VTG expression was the highest in the hepatopancreas and ovaries, and tissues. VTG expression significantly increased in the ovaries and hepatopancreas after 24 h exposure to DEHP. Increased responses of VTG transcripts were found in M. japonicus exposed to DEHP and BPA for 96 h; however, VTG expression decreased in both tissues after irgarol exposure. After an exposure of 7 d, VTG expression significantly increased in the ovaries and hepatopancreas for all concentrations of all chemicals. These results suggest that the crustacean embryogenesis and endocrine processes are impaired by the environmental chemical pollutants DEHP, BPA, and irgarol.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

Park

Kim

et al. 2019

Applied Sciences

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“…PPARs exert the majority of their effects as heterodimers with the retinoid x receptor (RXR) and are some of the key regulators of lipid and carbohydrate metabolism that also regulate inflammatory responses [Reviewed in (104)]. In African sharptooth catfish ( Clarias gariepinus ), exposure to DEHP led to dysregulation of PPAR gene expression and protein levels which consequently affects lipid homeostasis (105). Expression of genes related to lipid accumulation, oxidative stress and proteolysis were altered in female rare minnow ( Gobiocypris rarus ) following exposed to different doses of BPA in a non-monotonic pattern (38).…”

Section: Discussionmentioning

confidence: 99%

Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

et al. 2018

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Environmental contaminants are known to impair reproduction, metabolism and development in wild life and humans. To investigate the mechanisms underlying adverse effects of contaminants, fathead minnows were exposed to a number of endocrine disruptive chemicals (EDCs) including Nonylphenol (NP), bisphenol-A (BPA), Di(2-ethylhexyl) phthalate (DEHP), and a mixture of the three chemicals for 21 days, followed by determination of the liver transcriptome by expression microarrays. Pathway analysis revealed a distinct mode of action for the individual chemicals and their mixture. The results showed expression changes in over 980 genes in response to exposure to these EDC contaminants individually and in mixture. Ingenuity Pathway core and toxicity analysis were used to identify the biological processes, pathways and the top regulators affected by these compounds. A number of canonical pathways were significantly altered, including cell cycle & proliferation, lipid metabolism, inflammatory, innate immune response, stress response, and drug metabolism. We identified 18 genes that were expressed in all individual and mixed treatments. Relevant candidate genes identified from expression microarray data were verified using quantitative PCR. We were also able to identify specific genes affected by NP, BPA, and DEHP individually, but were also affected by exposure to the mixture of the contaminants. Overall the results of this study provide novel information on the adverse health impact of contaminants tested based on pathway analysis of transcriptome data. Furthermore, the results identify a number of new biomarkers that can potentially be used for screening environmental contaminants.

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“…As reported by other scholars such as Johnson et al (2009), Kosai et al (2011, Sadekarpawar and Parikh (2013), Dhanasekar et al (2018) that due to the dynamic of testes and high cell turnover its more vulnerable to a wide variety of chemical toxicants that elevated the occurrence of intersex in wild fish populations associated with exposure to human wastewater. Furthermore, Bin-Dohaish (2012) and Adeogun et al (2018;2019) mentioned that, synthetic products such as biphenol A, polychlorinated biphenol, dioxins, phtalates, pesticides, heavy metals, alkyphenols, polycyclic aromatic hydrocarbons, ethinylestradiol and estradiol. Such compounds, among others, that seeping from sewage water to aquatic environments are associated with observation of testis-ova in males and changes in secondary sex characteristics of male and female fish.…”

Section: Discussionmentioning

confidence: 99%

Histological Assessment of Gonadal Alteration in Wild Nile Tilapia Oreochromis niloticus Inhabiting Lake Manzala, Egypt

Amer¹,

Ahmed²

2019

Egypt. J. of Aquatic Biolo. and Fish.

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The aim of the study was to detect the detrimental effects of water pollutants on gonads structure of wild tilapia, O. niloticus. A total of 209 wild fish were collected from Lake Manzala during the spawning season, summer of 2018. According to the sex ratio and the body weigh fish were divided into four male and three female groups. Anatomical measurements and histological examination were performed for all specimens. Data reveled that sex ratio of collected fish was 74.64 and 25.36 for male and female, respectively. Body length for males was ranged from 11.6 to 19.5 cm and from 13.6 to 18.58 cm for females. Body weight was ranged from 30.05 to 139.73 g and from 39.60 to 125.13 g for male and female, respectively. Microscopic inspection illustrated that fish gonads both testes and ovaries were affected as indicated by the deterioration and degeneration of seminiferous tubules and germinal epithelium as well as the existent of testis-ova and testis fibrosis, and in female by increasing the percentage of atretic follicles and reabsorption of vitellogenic oocytes. The data reflected the prominent changes in male and female gonadal structure, which could be attributed to the accumulation of pollutants and prolonged period of exposure. This may affect the future spawning seasons; the rate of sex cycles or reproductive failure eventually. Such changes can damage the economic revenue of Lake Manzala. Thus, it is recommended to increase water circulation through inlet purgation to attenuate pollutants and restocking the lake.Amer M. A. and Ahmed, K. M. 186

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Endocrine disruptor responses in African sharptooth catfish (Clarias gariepinus) exposed to di-(2-ethylhexyl)-phthalate

Cited by 21 publications

References 77 publications

Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

Environmental Pollutants Impair Transcriptional Regulation of the Vitellogenin Gene in the Burrowing Mud Crab (Macrophthalmus Japonicus)

Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

Histological Assessment of Gonadal Alteration in Wild Nile Tilapia Oreochromis niloticus Inhabiting Lake Manzala, Egypt

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