Endocrine disruption by several aniline derivatives and related mechanisms in a human adrenal H295R cell line and adult male zebrafish

Bhuiyan, Nurul Huda; Kang, Habyeong; Kim, Ji Hyun; Kim, Sung-Min; Kho, Younglim; Choi, Kyungho

doi:10.1016/j.ecoenv.2019.05.003

Cited by 21 publications

(10 citation statements)

References 45 publications

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“…The 14-day fish assay was carried out according to Organization for European Economic Cooperation (OECD) Guideline No. 204 (fish prolonged toxicity test: 14-day exposure) with minor modifications [38,39]. Sixteen male zebrafish (four male fish per 3 L beaker with four replicates) were assigned to each treatment.…”

Section: Zebrafish Culture and Exposurementioning

confidence: 99%

“…As the housekeeping gene, 60S ribosomal protein L8 [RPL8] and beta-actin [β-ACTIN] were chosen for zebrafish and H295R cells, respectively. The primer sequences of the target genes used in the study were determined based on previous studies [36,39]. To isolate total RNA for zebrafish liver and H295R cells, the collected samples were extracted using the RNeasy mini kit (Qiagen, Hilden, Germany).…”

Section: Measurement Of Gene Expressionmentioning

confidence: 99%

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Investigation on Sex Hormone-Disruption Effects of Two Novel Brominated Flame Retardants (DBDPE and BTBPE) in Male Zebrafish (Danio rerio) and Two Human Cell Lines (H295R and MVLN)

et al. 2021

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Decabromodiphenyl ethane (DBDPE) and 1,2-bis(2,4,6-tribromo-phenoxy) ethane (BTBPE) are novel brominated flame retardants (NBFRs) and have been detected in variety of environment and biota. Although sex endocrine-disrupting potential has been suggested in experimental studies, their adverse effects on sex steroid hormones and underlying mechanisms are largely unclear. The purpose of the present study is to investigate the sex hormone-disrupting effects of two NBFRs using in vivo and in vitro models together. For this, male zebrafish (Danio rerio) along with human adrenocortical carcinoma (H295R) and breast carcinoma (MVLN) cell lines were employed. In male zebrafish, 14-day exposure to DBDPE significantly increased 17β-estradiol (E2) concentrations. Disruption of sex hormone regulation was also suggested after exposure to BTBPE, i.e., the increasing trend of E2 levels, E2/11-ketotestosterone (11-KT) ratio, and estrogen receptor-alpha (erα) and erβ gene expression levels. In H295R cells, an E2/T ratio showed an increasing trend by DBDPE exposure, but transcriptions of major genes in steroidogenesis pathway were not affected. Taken together, our observation implies that two NBFRs could cause the sex hormone disruption potential in male zebrafish and H295R cells but probably not through alteration of steroidogenesis pathway.

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Section: Zebrafish Culture and Exposurementioning

confidence: 99%

Section: Measurement Of Gene Expressionmentioning

confidence: 99%

Investigation on Sex Hormone-Disruption Effects of Two Novel Brominated Flame Retardants (DBDPE and BTBPE) in Male Zebrafish (Danio rerio) and Two Human Cell Lines (H295R and MVLN)

et al. 2021

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“…However, according to ISO/TR 16178 (2012), aniline derivatives in rubber are considered category 5 critical substances, which are suspected to have an effect on the wearer. Further, they are potentially hazardous materials that can be inhaled or absorbed into the human body through the skin, which can cause tissue hypoxia, met-hemoglobin, and damage to the central nervous system and cardiovascular system (Ryzhakov 2018;Bhuiyan et al 2019;Chaturvedi and Katoch 2020). Moreover, waste rubber products that contain aniline derivative residues cause soil and water pollution and affect the growth and reproduction of aquatic organisms (Miskam et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Novel poly(β-cyclodextrin) porous material as solid phase extraction sorbent for aniline derivatives in rubber samples

Dai¹,

Yin

et al. 2021

BioRes

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Aniline derivates are widely used in rubber products as anti-aging agents, but their biological toxicity causes harm in the use and recycling of rubber products. Therefore, in this study, a high-performance poly(3,4,5,6-tetra-fluorophthalonitrile-β-cyclodextrin)(P-TFPN-β-CD) solid-phase extraction (SPE) adsorbent was synthesized and applied to extract and detect aniline derivatives in rubber specimens. After crosslinking with 3,4,5,6-tetrafluorophthalonitrile (TFPN), the polymer exhibited mesopores (7.88 nm) and a large specific area (55.2 m2/g). The porous structure significantly improved the extraction efficiency (recovery was between 90.1% and 110.5%) and rate (60% in 10 s) of 4 aniline derivatives (aniline, N-ethyl aniline, 1,2-phenylenediamine, and p-phenylenediamine). With a combined gas chromatographic-flame ionization detector (GC-FID), the extracted aniline derivatives were accurately detected (RSD = 1.60% to 9.90%). Due to the weak interaction between P-TFPN-β-CD and analyte, the prepared poly-TFPN-β-CD SPE adsorbent was regenerated via mild washing with methanol. The high absorption capacities remained after 4 extracting-washing cycles. The novel porous SPE adsorbent showed good extracting and recycling performance for the analysis and detection of aniline compounds in rubber. Thus, it has good prospects for application to improve detection efficiency and reduce cost.

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“…Zebrafish, as an animal model has many advantages including rapid propagating and high homology with human genes, has been successfully applied in developmental biology, genetics and human diseases studies [8][9][10]. In fact, zebrafish are especially suitable for the study of thrombus due to ease observation of circulation system without invasive methods and its good response to anti-coagulant drugs commonly used in clinical treatment [11,12].…”

Section: Introductionmentioning

confidence: 99%

Antithrombotic effect and action mechanism of Salvia miltiorrhiza and Panax notoginseng herbal pair on the zebrafish

et al. 2020

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Background: Salvia miltiorrhiza (Danshen, DS) and Panax notoginseng (Sanqi, SQ) are famous traditional Chinese herbs, and their herbal pair (DS-SQ) has been popular used as anti-thrombotic medicines. However, there is still a lack of sufficient scientific evidence to illustrate the optimum combination ratio of these two herbs as well as its action mechanisms. The purpose of this study is to investigate the anti-thrombotic effects of DS-SQ on zebrafish and explore its possible action mechanism. Methods: Firstly, the chemical components in DS-SQ extract were analyzed by LC-ESI-MS/MS. Then, a phenylhydrazine (PHZ)-induced zebrafish thrombosis model was developed for evaluating the anti-thrombotic effects of DS-SQ extracts with different combination ratios and their nine pure compounds. Followed, Real-time quantitative PCR (RT-qPCR) assays were performed to investigate the potential antithrombotic mechanisms of DS-SQ. Results: Thirty-three components were tentatively identified by LC-MS analysis. DS-SQ at the ratio of 10:1 presented the best anti-thrombotic effect, and rosmarinic acid, lithospermic acid and salvianolic acid B of DS showed good antithrombotic activity on zebrafish thrombosis model. The RT-qPCR assays indicated that DS-SQ (10:1) could cure the PHZ-induced thrombosis by downregulating the expression of PKCα, PKCβ, fga, fgb, fgg and vWF in zebrafish. Conclusions: DS-SQ with the combination ratio of 10:1 showed optimum anti-thrombotic effect on PHZ-induced zebrafish thrombosis model, which provided a reference for reasonable clinical applications of DS-SQ herbal pair.

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Endocrine disruption by several aniline derivatives and related mechanisms in a human adrenal H295R cell line and adult male zebrafish

Cited by 21 publications

References 45 publications

Investigation on Sex Hormone-Disruption Effects of Two Novel Brominated Flame Retardants (DBDPE and BTBPE) in Male Zebrafish (Danio rerio) and Two Human Cell Lines (H295R and MVLN)

Investigation on Sex Hormone-Disruption Effects of Two Novel Brominated Flame Retardants (DBDPE and BTBPE) in Male Zebrafish (Danio rerio) and Two Human Cell Lines (H295R and MVLN)

Novel poly(β-cyclodextrin) porous material as solid phase extraction sorbent for aniline derivatives in rubber samples

Antithrombotic effect and action mechanism of Salvia miltiorrhiza and Panax notoginseng herbal pair on the zebrafish

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