Endocrine disrupting chemicals in fish: Developing exposure indicators and predictive models of effects based on mechanism of action

Ankley, Gerald T.; Bencic, David C.; Breen, Michael S.; Collette, Timothy W.; Conolly, Rory B.; Denslow, Nancy D.; Edwards, Stephen W.; Ekman, Drew R.; García-Reyero, Natàlia; Jensen, Kathleen

doi:10.1016/j.aquatox.2009.01.013

Cited by 227 publications

(153 citation statements)

References 47 publications

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“…The present study was conducted to enhance the interpretation of toxicogenomic data generated in experiments with small, asynchronous-spawning fish species (e.g., Ankley et al, 2009). The intent was to identify groups of genes whose expression was altered by differences in gross ovarian stage, specifically, the relative proportions of different-staged oocytes within the ovary, in the absence of any chemical stressor.…”

Section: Introductionmentioning

confidence: 99%

Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissue

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissue

et al. 2010

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“…Due to its varied effects on the vertebrate hypothalamic-pituitary-gonadal (HPG) axis, prochloraz has been used as a model compound for studies focused on different mechanisms of endocrine disruption (Gray et al, 2006;USEPA, 2007). Ankley et al (2009) describe a research effort focused on a systems-based approach for studying reproductive effects of HPG-active chemicals with differing mechanisms of action in fish. The HPG axis is a highly dynamic system, which, through various feedback mechanisms, strives to maintain physiological conditions conducive to reproduction even in potentially stressful situations.…”

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confidence: 99%

Dynamic Nature of Alterations in the Endocrine System of Fathead Minnows Exposed to the Fungicide Prochloraz

Ankley

Bencic

Cavallin

et al. 2009

Toxicological Sciences

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The vertebrate hypothalamic-pituitary-gonadal (HPG) axis is controlled through various feedback mechanisms that maintain a dynamic homeostasis in the face of changing environmental conditions, including exposure to chemicals. We assessed the effects of prochloraz on HPG axis function in adult fathead minnows (Pimephales promelas) at multiple sampling times during 8-day exposure and 8-day depuration/recovery phases. Consistent with one mechanism of action of prochloraz, inhibition of cytochrome P450 (CYP) 19 aromatase activity, the fungicide depressed ex vivo ovarian production and plasma concentrations of 17b-estradiol (E2) in female fish. At a prochloraz water concentration of 30 mg/l, inhibitory effects on E2 production were transitory and did not persist during the 8-day exposure phase. At 300 mg/l prochloraz, inhibition of E2 production was evident throughout the 8-day exposure but steroid titers recovered within 1 day of cessation of exposure. Compensation or recovery of steroid production in prochloraz-exposed females was accompanied by upregulation of several ovarian genes associated with steroidogenesis, including cyp19a1a, cyp17 (hydroxylase/lyase), cyp11a (cholesterol side-chain cleavage), and follicle-stimulating hormone receptor. In male fathead minnows, the 8-day prochloraz exposure decreased testosterone (T) production, possibly through inhibition of CYP17. However, as for E2 in females, ex vivo testicular production and plasma concentrations of T recovered within 1 day of stopping exposure. Steroidogenic genes upregulated in testis included cyp17 and cyp11a. These studies demonstrate the adaptability of the HPG axis to chemical stress and highlight the need to consider the dynamic nature of the system when developing approaches to assess potential risks of endocrine-active chemicals.

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“…This technique enabled the researchers to separate the fluorescently dyed proteomes of two samples (control and exposure treatments) within one gel, thereby reducing the gel-to-gel variation that inherently increases the error associated with conventional 2DGE expression analysis. Developed over a decade ago (1997; [20]), DiGE has not been readily used in aquatic toxicology, although Ankley et al [21] have proposed using the method within their framework for developing biomarkers of endocrine disruption. Liquid chromatography (LC) offers another means of proteome separation that is likewise rarely used with model aquatic species, although it offers several advantages over 2DGE.…”

Section: Model Fish Speciesmentioning

confidence: 99%

Review of recent proteomic applications in aquatic toxicology

Sanchez

Ralston-Hooper

Sepúlveda

2010

Enviro Toxic and Chemistry

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Abstract-Over the last decade, the environmental sciences have witnessed an incredible movement towards the utilization of highthroughput molecular tools that are capable of detecting simultaneous changes of hundreds, and even thousands, of molecules and molecular components after exposure of organisms to different environmental stressors. These techniques have received a great deal of attention because they not only offer the potential to unravel novel mechanisms of physiological and toxic action but are also amenable to the discovery of biomarkers of exposure and effects. In this article, we review the state of knowledge of one of these tools in ecotoxicological research: proteomics. We summarize the state of proteomics research in fish, and follow with studies conducted with aquatic invertebrates. A brief discussion on proteomic methods is also presented. We conclude with some ideas for future proteomic studies with fish and aquatic invertebrates. Environ. Toxicol. Chem. 2011;30:274-282. # 2010 SETAC

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Endocrine disrupting chemicals in fish: Developing exposure indicators and predictive models of effects based on mechanism of action

Cited by 227 publications

References 47 publications

Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissue

Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissue

Dynamic Nature of Alterations in the Endocrine System of Fathead Minnows Exposed to the Fungicide Prochloraz

Review of recent proteomic applications in aquatic toxicology

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