Endocrine Disrupting Chemicals: Human Exposure and Health Risks

Yang, Mihi; Park, Mi Seon; Lee, Ho-Sun

doi:10.1080/10590500600936474

Cited by 129 publications

(73 citation statements)

References 132 publications

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“…Besides the favourable effects that sport supplements may have on body composition, the potential ED substances present in these products may also have undesirable effects. The list of detrimental effects they may cause in humans and their progeny is described in many reviews and clinical studies providing links with infertility, heart problems and different types of carcinoma [8,[30][31][32]. Furthermore, of concern is that this study obtained a positive hormonal response in more then 70% of the tested products.…”

Section: Discussionmentioning

confidence: 93%

The application of reporter gene assays for the detection of endocrine disruptors in sport supplements

Plotan

Elliott²,

Scippo³

et al. 2011

Analytica Chimica Acta

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. a b s t r a c tThe increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement market, including sport supplements, is growing across the Developed World. Therefore, the need to ensure the quality and safety of sport supplements for the consumer is essential. The development and validation of two reporter gene assays coupled with solid phase sample preparation enabling the detection of estrogenic and androgenic constituents in sport supplements is reported. Both assays were shown to be of high sensitivity with the estrogen and androgen reporter gene assays having an EC 50 of 0.01 ng mL −1 and 0.16 ng mL −1 respectively.The developed assays were applied in a survey of 63 sport supplements samples obtained across the Island of Ireland with an additional seven reference samples previously investigated using LC-MS/MS. Androgen and estrogen bio-activity was found in 71% of the investigated samples. Bio-activity profiling was further broken down into agonists, partial agonists and antagonists. Supplements (13) with the strongest estrogenic bio-activity were chosen for further investigation. LC-MS/MS analysis of these samples determined the presence of phytoestrogens in seven of them. Supplements (38) with androgen bio-activity were also selected for further investigation. Androgen agonist bio-activity was detected in 12 supplements, antagonistic bio-activity was detected in 16 and partial antagonistic bio-activity was detected in 10. A further group of supplements (7) did not present androgenic bio-activity when tested alone but enhanced the androgenic agonist bio-activity of dihydrotestosterone when combined.The developed assays offer advantages in detection of known, unknown and low-level mixtures of endocrine disruptors over existing analytical screening techniques. For the detection and identification of constituent hormonally active compounds the combination of biological and physio-chemical techniques is optimal.

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Section: Discussionmentioning

confidence: 93%

The application of reporter gene assays for the detection of endocrine disruptors in sport supplements

Plotan

Elliott²,

Scippo³

et al. 2011

Analytica Chimica Acta

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“…EDCs are linked to birth defects, decreased fertility, unbalanced sex hormones, defects of the immune system, and neuronal adverse effects 1, 2, 3. The most investigated endocrine disruptor, diethylstilbestrol (DES), is a synthetic nonsteroidal estrogen used in the United States and Europe from the mid‐1940s to the 1970s for the prevention of spontaneous abortion and premature delivery.…”

mentioning

confidence: 99%

Suppression of liver Apo E secretion leads to HDL/cholesterol immaturity in rats administered ethinylestradiol

Yamaguchi

Ishii

Maeda³

et al. 2016

FEBS Open Bio

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Ethinylestradiol (EE), a main component of the combined oral contraceptive pill, is associated with an increased risk of arterial diseases. However, the toxicity mechanism of EE is poorly understood. In this study, we found that the exposure to EE reduced the serum apolipoprotein E (Apo E) level and high‐density lipoprotein (HDL)/cholesterol concentration in adult female rats. Diethylstilbestrol showed the same effects and both reductions were suppressed by coadministration of tamoxifen (TAM). Liver perfusion experiments revealed that the secretion rate of Apo E from the liver was significantly reduced. It is concluded that EE damages the maturation of HDL/cholesterol by delaying Apo E secretion from the liver, and this may lead to an increased risk of arterial diseases, such as atheromas.

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“…Most studies on adverse health effects of environmental contaminants have focused mainly on agricultural or industrial pesticides [2], heavy metals [3], and toxic industrial wastes and residues [2]. One large class of chemicals that has received little attention in this context comprises residues of pharmaceutical products (PPs), which are used in human and veterinary medicine in quantities comparable to those of the agrochemicals.…”

Section: Introductionmentioning

confidence: 99%

Development of an enzyme‐linked immunosorbent assay and a beta‐1 adrenergic receptor–based assay for monitoring the drug atenolol

Sapir

Shalev

Skalka

et al. 2013

Enviro Toxic and Chemistry

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Abstract-Two approaches for monitoring atenolol (ATL) were applied: an immunochemical assay and a competitive-binding assay, based on the interaction between ATL and its target receptor, b1 adrenergic receptor (b1AR). Polyclonal antibodies (Abs) for ATL were generated, and a highly specific microplate immunochemical assay, that is, an enzyme-linked immunosorbent assay (ELISA), for its detection was developed. The ATL ELISA exhibited I50 and limit of detection (I20) values of 0.15 AE 0.048 and 0.032 AE 0.016 ng/ml, respectively, and the Abs did not cross-react with any of the tested beta-blocker drugs. Furthermore, a human b1AR (h-b1AR) was stably expressed in Spodoptera frugiperda cells (Sf9). The receptor was employed to develop a competitive-binding assay that monitored binding of ATL in the presence of isoproteranol by quantification of secondary messenger, cyclic adenosine monophosphate (cAMP), levels in the transfected cells. The assay showed that the recombinant h-b1AR was functional, could bind the agonistic ligand isoproterenol as well as the antagonist ATL, as indicated by a dose-dependent elevation of cAMP in the presence of isoproteranol, and decrease after ATL addition. The highly efficient and sensitive ELISA and the receptor assay represent two methods suitable for efficient and cost-effective large-scale, high-throughput monitoring of ATL in environmental, agricultural, and biological samples. Environ.

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Endocrine Disrupting Chemicals: Human Exposure and Health Risks

Cited by 129 publications

References 132 publications

The application of reporter gene assays for the detection of endocrine disruptors in sport supplements

The application of reporter gene assays for the detection of endocrine disruptors in sport supplements

Suppression of liver Apo E secretion leads to HDL/cholesterol immaturity in rats administered ethinylestradiol

Development of an enzyme‐linked immunosorbent assay and a beta‐1 adrenergic receptor–based assay for monitoring the drug atenolol

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