Endocrine disrupting activity in fruits and vegetables evaluated with the E-screen assay in relation to pesticide residues

Schilirò, Tiziana; Gorrasi, Ilaria Silvia Rossella; Longo, A.; Coluccia, Sara; Gilli, Giorgio

doi:10.1016/j.jsbmb.2011.03.002

Cited by 24 publications

(18 citation statements)

References 57 publications

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“…These authors reported that a relative proliferative effect (RPE) ≥ 80 corresponds to estrogenic activity and suggest that the compound may have agonistic activity to ERα. However, it is worthwhile to highlight that the MCF-7 cells can elicit an estrogen-induced response involving both genomic and non-genomic pathways [25]. Hence, such as in in vivo condition, the MCF-7 cells proliferation observed in E-screen assay is not limited to the activation of ERs [23].…”

Section: Discussionmentioning

confidence: 99%

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Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Zingue

Tchoumtchoua

Ntsa

et al. 2016

BMC Complement Altern Med

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Background Millettia macrophylla was previously reported to have estrogenic effects and to prevent postmenopausal osteoporosis in Wistar rats. So, the study deals with the identification of its secondary metabolites and the evaluation of their estrogenicity and cytotoxicity toward tumoural cells. Thus, 13 known compounds were obtained from successive chromatographic columns and identified by NMR data compared to those previously reported.MethodsIn vitro estrogenicity of the isolates and the phenolic fraction (PF) of M. macrophylla were performed by E-screen and reporter gene assays, while their cytotoxicity was evaluated by Alamar Blue (resazurin) assay. A 3-days uterotrophic assay and the ability of PF to alleviate hot flushes in ovariectomized adult rats were tested in vivo.ResultsSeven of the 13 secondary metabolites turned to be estrogenic. Only two exhibited cytotoxic effects on MCF-7 and MDA-MB-231 with CC50 values of 110 μM and 160 μM, respectively. PF induced a significant (p < 0.01) MCF-7 cells proliferation and transactivated both ERα and ERβ in the reported gene assay at 10−2 μg/mL. In vivo, PF acted more efficiently than the methanol crude extract, resulting to a significant (p < 0.01) increase in the uterine wet weight, uterine protein level, uterine and vaginal epithelial height at the dose of 10 mg/kg BW. In addition, PF reduced the average duration and frequency of hot flushes induced in rat.ConclusionThese aforementioned results indicate that PF is a good candidate for the preparation of an improved traditional medicine able to alleviate some menopausal complaints such as vaginal dryness and hot flushes.Graphical abstractEstrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth. (Fabaceae): towards a better understanding of its underlying mechanism Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1385-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…This parameter was calculated according to Schiliro’et al [25], and is the ratio between the highest cell number achieved with the sample or 17β-estradiol and the cell number in the solvent control (0.01 % DMSO): PE = max cell number of sample/cell number of DMSO control .…”

Section: Methodsmentioning

confidence: 99%

Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Zingue

Tchoumtchoua

Ntsa

et al. 2016

BMC Complement Altern Med

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“…Finally, aliquots were read in a Biotek EL800 Multiscan apparatus (Winoosky, USA) at 510 nm. The estrogenic activity results were expressed as mean ± standard error of mean (SEM) of the proliferative effect (PE), which was calculated according to Schiliro´et al [29]: PE = max cell number of sample/cell number of DMSO control . The estrogenic activity of a sample was determined as the relative proliferative effect (RPE%).…”

Section: Methodsmentioning

confidence: 99%

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Zingue

Nde

Michel

et al. 2017

BMC Complement Altern Med

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BackgroundSince the biological properties of propolis depend to the plants that can be found in a specific region, propolis from unexplored regions attracts the attention of scientists. Ethanolic extract of Cameroonian propolis (EEP) is used to treat various ailments including gynecological problems and amenorrhea. Since there were no scientific data to support the above claims, the present study was therefore undertaken to assess estrogenic properties of Cameroonian propolis.MethodsTo achieve our goal, the ability of EEP to induce MCF-7 cells proliferation in E-screen assay as well as to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney cells (HEK293T) transfected with ERs was tested. Further, a 3-day uterotrophic assay was performed and the ability of EEP to alleviate hot flushes in ovariectomized adult rats was evaluated.ResultsIn vitro, EEP showed an antiestrogenic activity in both HEK293T ER-α and ER-β cells. In vivo, EEP induced a significant increase in a bell shape dose response manner of the uterine wet weight, the total protein levels in the uterus, the uterine and vaginal epithelium height and acini border cells of mammary gland with the presence of abundant eosinophil secretions. Moreover, EEP induced a significant decrease in the total number, average duration as well as frequency of hot flushes after 3 days of treatment in rat (equivalent to a month in woman). The dose of 150 mg/kg exhibited the most potent estrogenic effects among all the tested doses. The UPLC-HRMS analysis showed the presence of caffeic acid derivatives and trirtepernoids in EEP, which are well known endowed with estrogenic properties.ConclusionThese results suggest that Ethanolic extract of Cameroonian propolis has estrogen-like effects in vivo and may alleviate some menopausal problems such as vaginal dryness and hot flushes.Graphical abstractEthanol-extracted Cameroobian propolis exhibited in vitro and in vivo estrogen-like effects. This extract may contain promising phytoestrogens.

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“…Pape-Zambito et al (2012) reported concentrations of around 10 ng/L or Kg E 2 in dairy products and Courant et al (2008) concentrations of 34 ng/L E 2 (0.12 nM E 2 Eq/L) in milk. Schilirò et al (2011) reported values of 0.04, 1.59, 1.08, and 0.41 μg E 2 Eq100g in oranges, pineapples, kiwis, and tomatoes, respectively. Although E 2 and phytoestrogens in the foods and beverages may explain their estrogenicity, it has also been attributed to anthropogenic contamination.…”

Section: Introductionmentioning

confidence: 98%

Screening of hormone-like activities in bottled waters available in Southern Spain using receptor-specific bioassays

Real

Molina-Molina

Jiménez-Díaz

et al. 2015

Environment International

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Endocrine disrupting activity in fruits and vegetables evaluated with the E-screen assay in relation to pesticide residues

Cited by 24 publications

References 57 publications

Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Screening of hormone-like activities in bottled waters available in Southern Spain using receptor-specific bioassays

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