Endocrine Control of the Timing of Endometrial Sensitivity to a Decidual Stimulus

Finn, C. A.; Martin, L.

doi:10.1093/biolreprod/7.1.82

Cited by 170 publications

(130 citation statements)

References 7 publications

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“…Maximal proliferation of the stromal fibroblasts transformed to deciduomata in the rat requires a continued low level of estrogen along with an elevated level of progesterone (Yochim and DeFeo 1972). Administration of high estrogen doses, comparable to those secreted in estrus, results in a reduction in the mass of decidual tissue produced (Velardo and Hisaw 1951;Finn and Martin 1972). Neither the biochemical mechanism triggering decidualization nor the mechanism leading to decidual regression is known.…”

Section: Discussionmentioning

confidence: 99%

The variations of estrogen receptor, progesterone receptor, cAMP, ODC activity and RNA synthesis of deciduomata in pseudopregnant rats.

Hoshiai¹,

Uehara²,

Suzuki³

et al. 1982

Tohoku J. Exp. Med.

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Mass., USA HosHTAi, H., UEHARA, S., Suzuxi, M. and VILLEE, C.A. The Variations of Estrogen Receptor, Progesterone Receptor, CAMP, ODC Activity and RNA Synthesis of Deciduomata in Pseudopregnant Rats. Tohoku J. exp. Med., 1982, 137 (4), 349-360 The present study was done to define the conditions of implantation of fertilized ovum from serial day-to-day changes in the tissue weight of deciduomata in pseudopregnancy, estrogen receptor (ER), progesterone receptor (PR), CAMP content, RNA synthesizing capacity and ornithine decarboxylase activity (ODC). Pseudopregnancy was induced in Wistar rats in the estrus morning by vaginocervical stimulation. At the 4th day of pseudopregnancy deciduomata was induced by intraluminal instillation of olive oil with simultaneous oophorectomy and intramuscular injection of 20 mg progesterone depot (the day of induction of decidualization was arbitrarily expressed as D0 and the ensuing days, as D1, D2 and so on). The number of binding sites of ER and PR was estimated by exchange assay using dextran charcoal. cAMP content and RNA synthesizing capacity were determined by RIA and 3H-UTP uptake, respectively. ODC activity was measured by the method of Janne and Williams-Ashman. Following the induction of deciduomata the uterus weight (unilateral) increased abruptly to 2-fold at D2 and approximately 30-fold (4.5 g) at D8 and declined thereafter. ER diminished in a linear fashion from 2.77>< 10-10 M at D2 to 0.53 X 10-10 M at D,. On the other hand, PR was estimated 2.9 X 10-10 at D2, 2.5>< 10-10 M at D5 and decreased further at Ds and D,. cAMP was 77 nmoles/mg protein at D2 and decreased rapidly from D3 onward. RNA synthesizing capacity declined in a linear fashion from D3 to 1/2 at D5 and 1/3 at D,. ODC was found 1.7 CO2 nmoles/mg protein at D2, decreased at D3 and D4, but remained nearly the same at D4 and D5.-deciduomata; ODC activity; pseudopregnancy; hormone receptor; rats

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Section: Discussionmentioning

confidence: 99%

The variations of estrogen receptor, progesterone receptor, cAMP, ODC activity and RNA synthesis of deciduomata in pseudopregnant rats.

Hoshiai¹,

Uehara²,

Suzuki³

et al. 1982

Tohoku J. Exp. Med.

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“…In the nonlactating mouse, the development of the blastocyst takes place in an oestrogen-sensitized uterus (Finn & Martin, 1972) and results in elevated levels of embryonic RNA synthesis (Daentl & Epstein, 1971), protein synthesis (Epstein & Smith, 1973), and other metabolic activities (McLaren, 1973). In a lactating rodent (McLaren, 1968), or one which has been ovariectomized before the endogenous Oestrogen surge' and the pregnancy maintained with exogenous progesterone (rat: Psychoyos, 1967;mouse: Yoshinaga & Adams, 1966), the embryos enter an unsensitized uterus and become metabolically quiescent (McLaren, 1968;Weitlauf, 1973Weitlauf, ,1974a.…”

Section: Introductionmentioning

confidence: 99%

Uterine proteins and the activation of embryos from mice during delayed implantation

Pratt¹

1977

Reproduction

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Summary. Ovariectomy-induced delay of implantation was used to study the role of the uterine environment in controlling implantation in mice. Labelling studies in vivo showed that uterine protein synthesis and secretion is maximal 2\p=n-\5h and 24\p=n-\30h after the oestradiol injection which initiates implantation. Embryos removed from uteri 5,12 or 30 h after oestradiol injection were able to transport and utilize precursors of nucleic acids and proteins in short-term cultures at the same rate as normal embryos, although 'delayed' embryos had low levels of activity. These results suggest that 'delayed' embryos are metabolically activated within 5 h of release from delay, perhaps because of the hormonally-induced changes in uterine proteins which occur at this time.

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“…With the omission of oestrogen or progesterone or both, the DCR failed to develop, nor did it occur in intact virgin females untreated with exogenous hormones and which, of course, lacked corpora lutea (see Clarke et al, 1970). The regimen which has proved satisfactory for the bank vole is one that had been developed for the laboratory mouse (Finn & Martin, 1972) and successfully applied to the field vole (Milligan, 1974). The need for priming with oestrogen followed after 3 days by treatment with progesterone and very small amounts of oestrogen is an approximation to the likely endogenous endocrine changes which occur before and after mating.…”

Section: Discussionmentioning

confidence: 99%

Hormonal requirements and the influence of age on the decidual cell reaction in the bank vole (Clethrionomys glareolus)

Nerquaye-Tetteh¹,

Clarke²

1987

Reproduction

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Summary. Laboratory bred bank voles of different age and reproductive status were bilaterally ovariectomized, treated with exogenous oestrogen or progesterone or both, and given an artificial decidual stimulus (crushing with artery forceps). An hormonal regimen of oestrogen (100 ng) followed by progesterone (1 mg) and small amounts of oestrogen (10 ng) was necessary for maximal development of the decidual cell reaction (DCR). A DCR did not develop if the animals were treated with only oestrogen or progesterone. Middle-aged virgin females 5\p=n-\7months old had larger responses than did young virgin females 2\p=n-\3months old, and parous females, 15\p=n-\19months old and having had at least 10 litters, had a response similar to that of virgin females of about the same age. The size of the DCR in old parous females was inversely related to the time elapsing since the last litter. It is concluded that in its hormonal requirements for a successful DCR the bank vole is similar to the mouse. The results also suggest that the increase in fertility of bank voles up to the age of about 100 days may in part be explained by increased response of the endometrium to blastocysts. The reduction in size of the DCR in old animals is a consequence of ageing rather than of repeated pregnancies.

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Endocrine Control of the Timing of Endometrial Sensitivity to a Decidual Stimulus

Cited by 170 publications

References 7 publications

The variations of estrogen receptor, progesterone receptor, cAMP, ODC activity and RNA synthesis of deciduomata in pseudopregnant rats.

The variations of estrogen receptor, progesterone receptor, cAMP, ODC activity and RNA synthesis of deciduomata in pseudopregnant rats.

Uterine proteins and the activation of embryos from mice during delayed implantation

Hormonal requirements and the influence of age on the decidual cell reaction in the bank vole (Clethrionomys glareolus)

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