Endocrine Complications Following Radiotherapy and Chemotherapy for Nasopharyngeal Carcinoma

Darzy, Ken

doi:10.5772/32572

Cited by 2 publications

(1 citation statement)

References 81 publications

(117 reference statements)

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“…Patients with NPC often present in the late stages of the disease at the time of diagnosis ( 14 ). Radiotherapy is the primary modality of treatment for this type of cancer, however this type of therapy risks damage to the hypothalamic-pituitary axis due to the location of the nasopharynx ( 15 ). If GnRH has effects on NPC cells, alteration of secretion of GnRH may affect the progression of NPC.…”

Section: Introductionmentioning

confidence: 99%

Gonadotropin-releasing hormone inhibits the proliferation and motility of nasopharyngeal carcinoma cells

Teng

Ahmad

Sabaratnam

et al. 2015

Molecular Medicine Reports

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Gonadotropin-releasing hormone (GnRH), or its analogues have been demonstrated to exhibit anti-proliferative effects on tumour cells in ovarian, endometrial and breast cancer through GnRH-receptors (GnRH-R). However, the role of GnRH in nasopharyngeal carcinoma (NPC) remains to be elucidated. In order to investigate the effects of GnRH in NPC, the present study examined the expression of the GnRH-R transcript in NPC and investigated the phenotypic changes in HK1 cells, a recurrent NPC-derived cell line, upon receiving GnRH treatment. Firstly, the GnRH-R transcript was demonstrated in the NPC cell lines and four snap frozen biopsies using reverse transcription-quantitative polymerase chain reaction. In addition, immunohistochemistry revealed the expression of GnRH-R in two of the eight (25%) NPC specimens. Treatment with GnRH induced a rapid increase in intracellular ionised calcium concentration in the NPC cells. GnRH and its agonists, triptorelin and leuprolide, exerted anti-proliferative effects on the NPC cells, as determined using an MTS assay. GnRH did not induce any cell cycle arrest in the HK1 cells under the conditions assessed in the present study. Time-lapse imaging demonstrated a reduction in cell motility in the GnRH-treated cells. In conclusion, GnRH, or its analogues may have antitumour effects on NPC cells. The consequences of alterations in the levels of GnRH on the progression of NPC require further examination.

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Section: Introductionmentioning

confidence: 99%