Endocrine activity of persistent organic pollutants accumulated in human silicone implants — Dosing   assays by partitioning from silicone

Gilbert, Dorothea; Mayer, Philipp; Pedersen, Mikael; Vinggaard, Anne Marie

doi:10.1016/j.envint.2015.07.008

Cited by 17 publications

(14 citation statements)

References 48 publications

(58 reference statements)

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“…For a more sorptive chemical, hexachlorobenzene, Stadnicka-Michalak et al 7 found 2% and 8% left in medium with cells in duplicate measurements after 24 h incubation at 19 °C covered with plastic foil. This corresponds well with the 2−4% left after 24 h in the open and closed wells of the N2supplement medium in this study.…”

Section: Chemical Research In Toxicologymentioning

confidence: 99%

“…Passive dosing is a promising approach for this . It has mainly been used in larger volumes such as microplates with 24 wells and vials. ,,− However, designing passive dosing systems for in vitro tests is not straightforward because the tests require specific conditions relating to growth surface, growth medium, and CO 2 level and because contact between the cells and the passive dosing phase should be avoided (direct contact will make it hard to determine the exposure level). No such design has yet been demonstrated for well plates with 96, 384, or 1536 wells.…”

Section: Introductionmentioning

confidence: 99%

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Time-Resolved Freely Dissolved Concentrations of Semivolatile and Hydrophobic Test Chemicals in In Vitro Assays—Measuring High Losses and Crossover by Headspace Solid-Phase Microextraction

Birch

Kramer

Mayer

2019

Chem. Res. Toxicol.

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In vitro assays are normally conducted in plastic multi-well plates open to exchange with the ambient air. The concentration of test substances freely available to cells is often not known, can change over time, and is difficult to measure in the small volumes in microplates. However, even a well-characterized toxicological response is of limited value if it cannot be linked to a welldefined exposure level. The aim of this study was to develop and apply an approach for determining time resolved freely dissolved concentrations of semi-volatile and hydrophobic organic chemicals (SVHOCs) in in vitro assays: (1) free fractions were measured by a new medium dilution method and (2) time-resolved loss curves were obtained by measurements of total concentrations in 96-well plates during incubations at 37°C. Headspace solid-phase microextraction was used as an analytical technique for 24 model chemicals spanning 6 chemical groups and 4-5 orders of magnitude in K ow and K aw. Free fractions were above 30% for chemicals with log K ow < 3.5 and then decreased with increasing log K ow. Medium concentrations declined significantly (>50%) within 24 hours of incubation for all 20 chemicals having log K ow > 4 or log K aw >-3.5 in serum free medium. Losses of chemicals were lower for medium containing 10% fetal bovine serum, most significantly for chemicals with log K ow > 4. High crossover to neighboring wells was observed also below log K ow of 4 and log K aw of-3.5. Sealing the well plates had limited effect on the losses, but clearly reduced crossover. The high losses and crossover of most tested chemicals question the suitability of multi-well plates for in vitro testing of SVHOCs, and call for (1) test systems that minimize losses, (2) methods to control in vitro exposure, (3) analytical confirmation of exposure and (4) exposure control and confirmation being included in good in vitro reporting standards.

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Section: Chemical Research In Toxicologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Time-Resolved Freely Dissolved Concentrations of Semivolatile and Hydrophobic Test Chemicals in In Vitro Assays—Measuring High Losses and Crossover by Headspace Solid-Phase Microextraction

Birch

Kramer

Mayer

2019

Chem. Res. Toxicol.

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“…Contrarily, the drawback is that it leads to a single exposure level. While it is possible to vary the exposure level by concentrating or diluting the extract before loading the PD polymer, 33 for environmental mixtures this approach is associated with technical difficulties, for example, regarding sensitivity, in particular for less contaminated samples. In the following sections, we discuss the conditions for these two major strategies (i.e., total extraction/solvent spiking vs passive sampling/passive dosing) and combinations thereof (total extraction/passive dosing and passive sampling/solvent spiking) in the aquatic environment.…”

Section: Environmental Science and Technologymentioning

confidence: 99%

Strategies for Transferring Mixtures of Organic Contaminants from Aquatic Environments into Bioassays

Jahnke

Mayer

Schäfer

et al. 2016

Environ. Sci. Technol.

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“…Furthermore, in order to obtain a quantitative link between HBM data and in vitro outputs -thereby improving predictions -it would be relevant to correct the in vitro output for factors such as protein binding, evaporation, binding to test plates/tubes/pipettes etc. in order to obtain the true intracellular concentration [46,47].…”

Section: A Panel Of Human-based In Vitro Assaysmentioning

confidence: 99%

Chemical risk assessment based on in vitro and human biomonitoring data: A case study on thyroid toxicants

Johansson

Boberg

Dybdahl

et al. 2019

Current Opinion in Toxicology

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Today detailed risk assessment can only be performed for a few percent of the total number of current-use chemicals due to lack of data. Toxicity data is therefore needed for a substantial number of untested chemicals, a task that requires improved and faster chemical risk assessment strategies that are cost-efficient, human relevant and ethically responsible. In this commentary we use a case study on five known thyroid toxic chemicals (perfluorooctanesulfonic acid (PFOS), triclosan, tetrabromobisphenol A (TBBPA), decabromodiphenyl ether (BDE-209), and hexabromocyclododecane (HBCD)) to explore the use of in vitro data for hazard assessment together with human biomonitoring (HBM) data for exposure assessment when evaluating human risk. Based on the case study we conclude that in vitro and HBM data can be used for risk ranking of chemicals. We envision that an in vitro/HBM approach can use data from studies such as the big European initiative HBM4EU together with human relevant in vitro data to make alternative risk assessment more valuable to finally be able to 'stand-alone'.

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Endocrine activity of persistent organic pollutants accumulated in human silicone implants — Dosing assays by partitioning from silicone

Cited by 17 publications

References 48 publications

Time-Resolved Freely Dissolved Concentrations of Semivolatile and Hydrophobic Test Chemicals in In Vitro Assays—Measuring High Losses and Crossover by Headspace Solid-Phase Microextraction

Time-Resolved Freely Dissolved Concentrations of Semivolatile and Hydrophobic Test Chemicals in In Vitro Assays—Measuring High Losses and Crossover by Headspace Solid-Phase Microextraction

Strategies for Transferring Mixtures of Organic Contaminants from Aquatic Environments into Bioassays

Chemical risk assessment based on in vitro and human biomonitoring data: A case study on thyroid toxicants

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