Endochondral mineralization in cartilage organoid culture

Zimmermann, Bernd; Somogyi, H.; Wachtel, Hannes

doi:10.1016/0922-3371(90)90086-c

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…33 Furthermore, CM from mouse calvarial cells accelerated the mineralization of mouse cultured cartilage tissue. 34 CM from MLO-Y4 cells, mouse osteocyte-like cell line, stimulated mouse MSC differentiation into osteoblasts. 35 With regard to the effect of CM on cell proliferation, CM-HPL cells decreased the proliferation of rBMSCs; 31 CM-MC3T3-E1, osteoblastic cell line, did not induce the proliferation of mouse MSCs; 35 and CM-hOB promoted the proliferation of human breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, CM‐PC‐3 cells stimulated the calcification of human osteoblastic cell line, Tak‐10, in medium supplemented with prostatic acid phosphate and α‐glycerophosphate 33 . Furthermore, CM from mouse calvarial cells accelerated the mineralization of mouse cultured cartilage tissue 34 . CM from MLO‐Y4 cells, mouse osteocyte‐like cell line, stimulated mouse MSC differentiation into osteoblasts 35 .…”

Section: Discussionmentioning

confidence: 99%

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Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells

Mizuno

Ozeki

Shiba

et al. 2008

Journal of Periodontology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells

Mizuno

Ozeki

Shiba

et al. 2008

Journal of Periodontology

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“…Cartilage organ cultures have been used as experimental models for studies related to cartilage growth [38]. Recently, Schr6ter-Kermani et al [34] and Zimmerman et al [46] reported mineralization of cartilage formed by limb bud mesenchymal cells in organoid cultures. In addition, further observations have demonstrated the capacity of mammalian bone marrow cells [4] or fetal rat calvaria [29] to form cartilage in vitro.…”

Section: Introductionmentioning

confidence: 99%

In vitro differentiation and mineralization of cartilaginous nodules from enzymatically released rat nasal cartilage cells

Sautier

Nefussi

Forest

1993

Biology of the Cell

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Nasal cartilage cells from 21-day-old rat fetuses were cultured at high density in the presence of ascorbic acid and beta-glycerophosphate over a 12-day period. Immediately after plating, the cells exhibited a fibroblastic morphology, lost their chondrocyte phenotype and expressed type I collagen. On day 3, clusters of enlarged polygonal cells were found. These cell clusters synthetized type II collagen and formed an alcian-blue-positive matrix. The following days, a progressive increase in the number of cells positive for type II collagen was noted and, on day 8, typical cartilaginous nodules were formed. These nodules increased in size and number, spreading outward, laying down a dense matrix which mineralized. Light and electron microscopy observations of cross-sections of nodules confirmed the cartilaginous nature of this tissue formed in vitro with typical chondrocytes embedded in a hyaline matrix. Furthermore, at the electron microscopic level, matrix vesicles were seen in extracellular matrix associated with the initiation of mineralization. Typical rod-like crystals were present in the intercellular spaces along the collagen fibers. These results indicated that in a specific environment, dedifferentiated chondrocytes were able to redifferentiate and to form nodular structures with morphological ultrastructure of calcified cartilage observed in vivo.

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“…Although many types of mineralizing chondrocyte cultures have been described, these cultures use either growth plate chondrocytes, embryonic chondrocytes, or embryonic growth plate chondrocytes and not articular chondrocytes. It doesn't seem appropriate to extrapolate, to calcified cartilage, observations generated from these types of mineralizing cultures as these cultures use ceils that generate cartilage which serves as a template for bone formation (3,4,9,10,16,22,25,32,36), whereas the calcified zone of articular cartilage, in mature individuals after growth has ceased, appears to be hyaline cartilage which has undergone mineralization. Furthermore, during the process of mineralization, in vivo, those cell types synthesize an extracellular matrix that will be permeated by vascular ~To whom correspondence should be addressed at Department of Pathology, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada.…”

mentioning

confidence: 99%

In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

Kandel

Boyle

Gibson

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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Study of the deep articular cartilage and adjacent calcified cartilage has been limited by the lack of an in vitro culture system which mimics this region of the cartilage. In this paper we describe a method to generate mineralized cartilagenous tissue in culture using chondrocytes obtained from the deep zone of bovine articular cartilage. The cells were plated on Millipore CMR filters. The chondrocytes in culture accumulated extracellular matrix and formed cartilagenous tissue which calcified when beta-glycerophosphate was added to the culture medium. The cartilagenous tissue generated in vitro contains both type II and type X collagens, large sulfated proteoglycans, and alkaline phosphatase activity. Ultrastructurally, matrix vesicles were seen in the extracellular matrix. Selected area electron diffraction confirmed that the calcification was composed of hydroxyapatite crystals. The chondrocytes, as characterized thus far, appear to maintain their phenotype under these culture conditions which suggests that these cultures could be used as a model to examine the metabolism of cells from the deep zone of cartilage and mineralization of cartilagenous tissue in culture.

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Endochondral mineralization in cartilage organoid culture

Cited by 18 publications

References 29 publications

Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells

Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells

In vitro differentiation and mineralization of cartilaginous nodules from enzymatically released rat nasal cartilage cells

In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

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