“…For the purpose of our study, a co-extraction method for eCBs, NAEs and eiCs was developed to be amenable for small portions of the mouse brain region, e.g., 2-5 mg tissue weight, and importantly, pertaining to the rigorous criteria of the standard operating procedure concerning tissue handling, transport, extraction and storage conditions already developed in the lab for eCBs, in order to strictly control and reduce the artificial alterations of their endogenous levels among the controls and KA-induced epileptic seizure models [34,35,37,38]. This procedure combined with targeted quantification by liquid chromatography-multiple reaction monitoring (LC/MRM) with on-line polarity switching enables simultaneous eCB, NAE and eiC quantification (Fig.…”