Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties

Karlsson, Eva Nordberg; Schmitz, Eva; Linares-Pastén, Javier A.; Adlercreutz, Patrick

doi:10.1007/s00253-018-9343-4

Cited by 128 publications

(70 citation statements)

References 46 publications

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“…Carbohydrate degrading enzymes are also essentially used in the production pipelines of paper and food industries. In combination with other carbohydrate degrading enzymes, xylanases are utilized in bio-refineries, chemical and pharmaceutical industries, processing of fruit juices and production of prebiotics 3 – 8 .…”

Section: Introductionmentioning

confidence: 99%

High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca

Andaleeb

Ullah

Perbandt³

et al. 2020

Sci Rep

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Enzymatic degradation of vegetal biomass offers versatile procedures to improve the production of alternative fuels and other biomass-based products. Here we present the three-dimensional structure of a xylanase from Nectria haematococca (NhGH11) at 1.0 Å resolution and its functional properties. The atomic resolution structure provides details and insights about the complex hydrogen bonding network of the active site region and allowed a detailed comparison with homologous structures. Complementary biochemical studies showed that the xylanase can catalyze the hydrolysis of complex xylan into simple xylose aldopentose subunits of different lengths. NhGH11 can catalyze the efficient breakdown of beechwood xylan, xylan polysaccharide, and wheat arabinoxylan with turnover numbers of 1730.6 ± 318.1 min−1, 1648.2 ± 249.3 min−1 and 2410.8 ± 517.5 min−1 respectively. NhGH11 showed maximum catalytic activity at pH 6.0 and 45 °C. The mesophilic character of NhGH11 can be explained by distinct structural features in comparison to thermophilic GH11 enzymes, including the number of hydrogen bonds, side chain interactions and number of buried water molecules. The enzymatic activity of NhGH11 is not very sensitive to metal ions and chemical reagents that are typically present in associated industrial production processes. The data we present highlights the potential of NhGH11 to be applied in industrial biomass degradation processes.

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Section: Introductionmentioning

confidence: 99%

High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca

Andaleeb

Ullah

Perbandt³

et al. 2020

Sci Rep

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“…For instance, arabino-XOSs, including ␣-1,2and/or ␣-1,3-linked-Larabinofuranose substituent, and acidic XOSs, including ␣-1,2-linked-4-O-methyl-Dglucuronic acid (MeGlcA) substituent, can be generated from cereal arabinoxylan and hardwood glucuronoxylan, respectively. These branched XOSs also have a potential application in modulating gut microbiota (4)(5)(6). Furthermore, acidic XOSs are of interest as potential pharmaceutical agents due to their immunomodulating effects, prevention of iron deficiency anemia, and protection against the development of atopic dermatitis, etc.…”

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confidence: 99%

“…Xylan hydrolysis using endo-␤-1,4-xylanases (EC 3.2.1.8) is highly efficient and specific in XOS production, allowing a high control over the degree of polymerization (DP) and reduced production of undesired xylose and other by-products. Endoxylanases, belonging to glycoside hydrolase families 10 and 11 (GH10 and GH11, respectively) in the CAZy database (http://www.cazy.org/), have been well studied as major XOS-producing enzymes (4,6,(10)(11)(12). These enzymes can use both unsubstituted and substituted xylan as their substrate.…”

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confidence: 99%

GH30-7 Endoxylanase C from the Filamentous Fungus Talaromyces cellulolyticus

Nakamichi

Fujii

Fouquet³

et al. 2019

Appl Environ Microbiol

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Glycoside hydrolase family 30 subfamily 7 (GH30-7) enzymes include various types of xylanases, such as glucuronoxylanase, endoxylanase, xylobiohydrolase, and reducing-end xylose-releasing exoxylanase. Here, we characterized the mode of action and gene expression of the GH30-7 endoxylanase from the cellulolytic fungus Talaromyces cellulolyticus (TcXyn30C). TcXyn30C has a modular structure consisting of a GH30-7 catalytic domain and a C-terminal cellulose binding module 1, whose cellulose-binding ability has been confirmed. Sequence alignment of GH30-7 xylanases exhibited that TcXyn30C has a conserved Phe residue at the position corresponding to a conserved Arg residue in GH30-7 glucuronoxylanases, which is required for the recognition of the 4-O-methyl-α-d-glucuronic acid (MeGlcA) substituent. TcXyn30C degraded both glucuronoxylan and arabinoxylan with similar kinetic constants and mainly produced linear xylooligosaccharides (XOSs) with 2 to 3 degrees of polymerization, in an endo manner. Notably, the hydrolysis of glucuronoxylan caused an accumulation of 22-(MeGlcA)-xylobiose (U4m2X). The production of this acidic XOS is likely to proceed via multistep reactions by putative glucuronoxylanase activity that produces 22-(MeGlcA)-XOSs (XnU4m2X, n ≥ 0) in the initial stages of the hydrolysis and by specific release of U4m2X from a mixture containing XnU4m2X. Our results suggest that the unique endoxylanase activity of TcXyn30C may be applicable to the production of linear and acidic XOSs. The gene xyn30C was located adjacent to the putative GH62 arabinofuranosidase gene (abf62C) in the T. cellulolyticus genome. The expression of both genes was induced by cellulose. The results suggest that TcXyn30C may be involved in xylan removal in the hydrolysis of lignocellulose by the T. cellulolyticus cellulolytic system. IMPORTANCE Xylooligosaccharides (XOSs), which are composed of xylose units with a β-1,4 linkage, have recently gained interest as prebiotics in the food and feed industry. Apart from linear XOSs, branched XOSs decorated with a substituent such as methyl glucuronic acid and arabinose also have potential applications. Endoxylanase is a promising tool in producing XOSs from xylan. The structural variety of XOSs generated depends on the substrate specificity of the enzyme as well as the distribution of the substituents in xylan. Thus, the exploration of endoxylanases with novel specificities is expected to be useful in the provision of a series of XOSs. In this study, the endoxylanase TcXyn30C from Talaromyces cellulolyticus was characterized as a unique glycoside hydrolase belonging to the family GH30-7, which specifically releases 22-(4-O-methyl-α-d-glucuronosyl)-xylobiose from hardwood xylan. This study provides new insights into the production of linear and branched XOSs by GH30-7 endoxylanase.

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“…Furthermore, xylanase has been reported to improve Lactobacilli and Bifidobacteria counts in the caecum of broilers fed a corn/soy-based diet [ 28 ]; the study’s findings are in congruence with this study, as it indicates that LS also exerted xylanase activity. The reason the LS-produced xylanase has increases the population of Lactobacillus in the GIT of the broilers might be because the xylanase could produce xylooligosaccharides from xylan (a type of NSP that is abundant in WB) that acts as prebiotics [ 39 , 40 , 41 ]. Furthermore, according to the gene coding, xylosidase of GH3 and GH43 are present in the Lactobacillus genomes and GH43 is characterized by its high efficiency in utilizing xylobiose, xylotriose, and xylotetraose, which reasoned the promoted population of the Lactobacillus strains in the LS-supplemented group [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Laetiporus sulphureus-Fermented Wheat Bran on Growth Performance, Intestinal Microbiota and Digesta Characteristics in Broiler Chickens

Lin

Lee

2020

Animals

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This study investigated the effects of a Laetiporus sulphureus-fermented wheat bran (LS) supplementation on the microbiota and digesta characteristics of broiler chickens. Two hundred and forty male broilers (Ross 308) were randomly allocated into three groups fed with a corn–soybean-based diet (control), and the control diet being replaced with 5% wheat bran (WB) and 5% LS, respectively. Each group had four replicates and 20 birds per pen. Metagenomics analysis results of the ileum microbiota showed that, at the family level, the 5% LS groups had over 40% higher Lactobacillaceae compared to the control group in a mean difference comparison. Heat maps showed that, at the phylum level, the population of Firmicutes was higher and Proteobacteria was lower in the ileum of 5% LS compared to the control group. Results of the stack column plots of the top ten OTUs at the family level showed that a 5% LS and 5% WB supplementation altered the broiler microbiota distribution by increasing the relative abundance of Lactobacillaceae. Cecal microbiota analysis showed that the 5% LS-supplemented group had approximately 5% and 3% higher Veillonellaceae and Lactobacillaceae, respectively. Stack column plots of the top ten OTUs indicated that the distribution of cecal bacteria in each group was not markedly different. Both the ileum and cecum digesta in the 5% LS supplementation group had a slight and not significant elevation on the total VFA, while the pH values and ammonia nitrogen were significantly lowered compared to the control and 5% WB groups (p < 0.05). In addition, the 5% LS supplementation group had a significantly higher lactic acid concentration in both the ileum and cecum compared to the control and 5% WB groups (p < 0.05). In conclusion, a 5% LS supplementation could potentially enhance the feed conversion ratio and European Broiler Index (EBI) of broilers by elevating the family Lactobacillaceae and suppressing the phylum Proteobacteria’s population, thus creating changed intestinal environments that may potentially favor the growth and health of the broilers.

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Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties

Cited by 128 publications

References 46 publications

High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca

High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca

GH30-7 Endoxylanase C from the Filamentous Fungus Talaromyces cellulolyticus

Effects of Laetiporus sulphureus-Fermented Wheat Bran on Growth Performance, Intestinal Microbiota and Digesta Characteristics in Broiler Chickens

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