Endo-1,4-β-xylanase B from Aspergillus cf. niger BCC14405 Isolated in Thailand: Purification, Characterization and Gene Isolation

Abstract

During the screening of xylanolytic enzymes from locally isolated fungi, one strain BCC14405, exhibited high enzyme activity with thermostability. This fugal strain was identified as Aspergillus cf. niger based on its morphological characteristics and internal transcribed spacer (ITS) sequences. An enzyme with xylanolytic activity from BCC14405 was later purified and characterized. It was found to have a molecular mass of ca.

<b>Production of xylanases by an <i>Aspergillus niger</i> strain in wastes grain

“…These enzymes have applications in conversion of lignocellulosic substances to chemicals and fuels, animal feed digestion, food and textile industries, and as bleaching agents in the pulp and paper processing (KNOB et al, 2010;MOURE et al, 2006;POLIZELI et al, 2005). Filamentous fungi are widely used as enzyme producers and are generally considered as more potent xylanolytic enzymes producers than bacteria and yeast (KRISANA et al, 2005;POLIZELI et al, 2005).…”

Purification and characterization of an acid-stable and organic solvent-tolerant xylanase from Aspergillus awamori VTCC-F312

“…The purification factor for xylanases from Aspergillus strains was relatively low as 1.3-4 8,14 and also in this study, middle high: 30-33 10,15,20 or very high: 77-93 11 . The purification yield of the xylanase from A. awamori VTCC-F312 was the highest (22%) among purification yield reported; low as 3.4-4.5% (xylanase II 8 , xylanase I 11, 14 ), middle high as 10.5-14.8% (xylanase II 11 , xylanase I 8, 15 ), and high as 20.7% 9 . The specific activity of the xylanase in this study (217 U/mg) was higher than that (124 U/mg) of the A. niger C3486 xylanase 15 , relatively high as that (289 U/mg) of the xylanase from A. ficuum AF-98 U2/1 (490 U/mg) 12 and A. cf.…”

“…Xylanases from other Aspergillus strains were purified to homogeneity by using a similar purification scheme involving ammonium sulphate precipitation, gel filtration chromatography (Sephadex G-200, G-100, G-75), ion exchange chromatography (DEAESephadex A-50, DEAE Sepharose, Q-Sepharose), and affinity chromatography Phenyl Sepharose 6 Fast Flows, Sephacryl S-200 [8][9][10][11][12]15 . Xylanases have been purified to homogeneity with a molecular mass of 32-35 kDa from A. awamori 2B.361 U2/1 12 11 .…”

“…In the breeding, xylanase expedites the digestion process of food containing xylan and at the same time helps to reduce the viscosity in the digestive system followed by many positive effects such as improved food absorption, improved microorganism populations of the intestine in the advantageous direction, reduced digestion disorder, and drier excrement 5,6 . Among filamentous fungi, Trichoderma and Aspergillus have been widely studied for the xylanase production, and the genus Aspergillus is the most efficient producers of xylanolytic enzymes [7][8][9][10][11] . Thus for different applications, purification and biochemical characterization of xylanases from various Aspergillus strains have been carried out, including xylanases from A. awamori 12 , A. ficuum 10 , A. giganteus 8 , A. nidulans 7 , A. niger 3,9,[13][14][15] , and A. sydowii 11 .…”

“…Among filamentous fungi, Trichoderma and Aspergillus have been widely studied for the xylanase production, and the genus Aspergillus is the most efficient producers of xylanolytic enzymes [7][8][9][10][11] . Thus for different applications, purification and biochemical characterization of xylanases from various Aspergillus strains have been carried out, including xylanases from A. awamori 12 , A. ficuum 10 , A. giganteus 8 , A. nidulans 7 , A. niger 3,9,[13][14][15] , and A. sydowii 11 . In the previous study, cultural conditions for xy-lanase production by an A. awamori strain VTCC-F312 were optimized in shake flask cultures 16 and we developed a nutrient medium containing 6% (w/v) fish powder and 7% (w/v) of corn cobs for optimal xylanase production.…”