Endemik ve Tıbbi Bir Bitki Olan Hypericum adenotrichum Spach’ın In Vitro Çimlenme ve Fide Gelişimi

Abstract

In this study, it has been aimed to describe an appropriate procedure for in vitro seed germination and seedling development of Hypericum adenotrichum Spach which is an endemic plant under in vitro conditions. At he end of experiments, It has been found that seed germination of Hypericum adenotrichum is effected by stratification, photoperiod, dark, different temperature and Murashige Skoog media (MS) with macro salt ingradient in different levels (MS, ½ MS and ¼ MS) and vitamin additions (MS vitamins, B5 vitamins and Galzy vitamins) to these media. The highest germination value has been held when sterilized seeds were incubated in ¼ MS/Galzy nutrient medium under 16/8 photoperiod and 18 ºC conditions after stratification at 4ºC for 2 months. All seeds were observed during 8 weeks (22 ºC and 16/8 photoperiod) and radicula emergence was mostly in 7th days of incubation. At the end of these processes, parameters such as the highest number of shoots per seedling, mean number of lateral roots, mean length of shoots and number of leaves per shoot were evaluated by one-way analysis of variance and it has been concluded that ¼ MS/Galzy medium is the most proper media for seedling development.