Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s

Nguyen, To Nguyen Thi; Parry, Christopher M.; Campbell, James; Vinh, Phat Voong; Kneen, Rachel; Baker, Stephen

doi:10.1099/mgen.0.000861

Cited by 2 publications

(1 citation statement)

References 55 publications

(74 reference statements)

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“… 20 Plasmid pNG3 was recently described by Nguyen Thi Nguyen et al as an erythromycin resistance conferring plasmid in C. diphtheriae isolates from Vietnam. 21 Erythromycin treatment would then lead to co-selection of tetracycline resistance. Tetracycline resistance is prevalent in C. diphtheria e with 18% of resistant strains reported in the study of Hennart et al 20 …”

Section: Resultsmentioning

confidence: 99%

Novel tetracycline resistance gene tet(65) located on a multi-resistance Corynebacterium plasmid

Kittl,

Brodard,

Tresch

et al. 2024

Journal of Antimicrobial Chemotherapy

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Background Corynebacterium (C.) sp. 22KM0430 related to C. oculi and isolated from a dog exhibited resistance to tetracycline, and its WGS analysis revealed a putative resistance gene on a 35 562-bp plasmid also harbouring the MLSB resistance gene erm(X). Objectives To characterize the novel tetracycline resistance gene tet(65) and demonstrate its functionality by expression in C. glutamicum and Escherichia coli and plasmid curing of the host strain. Methods tet(65) was cloned with and without its repressor tetR(65) and expressed in C. glutamicum DSM20300 and E. coli DH5α. Plasmid was cured by non-selective passages. Minimal inhibitory concentrations (MICs) of tetracyclines were determined according to CLSI guidelines. Association of tet(65) with efflux was shown by the addition of reserpine to MIC assays. Phylogenetic position and transmembrane structure of Tet(65) were analysed using MEGA11 and DeepTMHMM. Results Tet(65) shows 73% amino acid identity with the closest related Tet(Z), contains 12 transmembrane domains and is structurally related to the Major Facilitator Superfamily. The tetracycline MICs decreased in the plasmid-cured strain and increased when tet(65) was expressed in C. glutamicum and in E. coli. The MICs of tetracycline decreased in the presence of reserpine indicating that tet(65) functions as an efflux pump. A GenBank search also identified tet(65) in C. diphtheriae and Brevibacterium (B.) casei and B. luteolum. Conclusions A novel tetracycline efflux gene tet(65) was identified in a C. oculi related species and was also present in the human pathogen C. diphtheriae and in Brevibacterium species indicating broader potential for dissemination.

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Section: Resultsmentioning

confidence: 99%

Novel tetracycline resistance gene tet(65) located on a multi-resistance Corynebacterium plasmid

Kittl,

Brodard,

Tresch

et al. 2024

Journal of Antimicrobial Chemotherapy

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Corynebacterium oculi-related bacterium may act as a pathogen and carrier of antimicrobial resistance genes in dogs: a case report

Tresch,

Watté,

Stengard

et al. 2023

BMC Vet Res

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Background The genus Corynebacterium comprises well-known animal and human pathogens as well as commensals of skin and mucous membranes. Species formerly regarded as contaminants are increasingly being recognized as opportunistic pathogens. Corynebacterium oculi has recently been described as a human ocular pathogen but has so far not been reported in dogs. Case presentation Here we present two cases of infection with a novel Corynebacterium sp., a corneal ulcer and a case of bacteriuria. The two bacterial isolates could not be identified by MALDI-TOF MS. While 16 S rRNA gene (99.3% similarity) and rpoB (96.6% identity) sequencing led to the preliminary identification of the isolates as Corynebacterium (C.) oculi, whole genome sequencing revealed the strains to be closely related to, but in a separate cluster from C. oculi. Antimicrobial susceptibility testing showed high minimal inhibitory concentrations of lincosamides, macrolides, tetracycline, and fluoroquinolones for one of the isolates, which also contained an erm(X) and tet-carrying plasmid as well as a nonsynonymous mutation leading to an S84I substitution in the quinolone resistance determining region of GyrA. Conclusions While the clinical signs of both dogs were alleviated by antimicrobial treatment, the clinical significance of these isolates remains to be proven. However, considering its close relation with C. oculi, a known pathogen in humans, pathogenic potential of this species is not unlikely. Furthermore, these bacteria may act as reservoir for antimicrobial resistance genes also in a One Health context since one strain carried a multidrug resistance plasmid related to pNG3 of C. diphtheriae.

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Endemic erythromycin resistant Corynebacterium diphtheriae in Vietnam in the 1990s

Cited by 2 publications

References 55 publications

Novel tetracycline resistance gene tet(65) located on a multi-resistance Corynebacterium plasmid

Novel tetracycline resistance gene tet(65) located on a multi-resistance Corynebacterium plasmid

Corynebacterium oculi-related bacterium may act as a pathogen and carrier of antimicrobial resistance genes in dogs: a case report

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