Endemic Angiostrongyliasis, Rio de Janeiro, Brazil

Simões, Raquel de Oliveira; Monteiro, Fernando A.; Sánchez, Elizabeth; Thiengo, Silvana Carvalho; Garcia, Juberlan Silva; Costa-Neto, Sócrates Fraga da; Luque, José L.; Maldonado, Arnaldo

doi:10.3201/eid1707.101822

Cited by 35 publications

(19 citation statements)

References 11 publications

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“…Recent studies have reported A. cantonensis infection in Brazil in Rattus rattus and R. norvergicus, with both acting as definitive hosts ( Simões et al 2011 , Moreira et al 2013 ). In the studied area, infected R. norvergicus were observed to have high prevalence and abundance of A. cantonensis and to contribute to dispersing the parasite to new areas ( Simões et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Angiostrongylus cantonensis infection in molluscs in the municipality of São Gonçalo, a metropolitan area of Rio de Janeiro, Brazil: role of the invasive species Achatina fulica in parasite transmission dynamics

Oliveira

Gentile

Júnior

et al. 2015

Mem. Inst. Oswaldo Cruz

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The aim of this study was to analyse the infection dynamics ofAngiostrongylus cantonensis in its possible intermediate hosts over two years in an urban area in the state of Rio de Janeiro where the presence ofA. cantonensis had been previously recorded in molluscs. Four of the seven mollusc species found in the study were exotic.Bradybaena similaris was the most abundant, followed byAchatina fulica, Streptaxis sp., Subulina octona, Bulimulus tenuissimus, Sarasinula linguaeformis and Leptinaria unilamellata. Only A. fulica and B. similaris were parasitised by A. cantonensis and both presented co-infection with other helminths. The prevalence of A. cantonensisin A. fulica was more than 50% throughout the study. There was an inverse correlation between the population size ofA. fulica and the prevalence of A. cantonensis and abundance of the latter was negatively related to rainfall. The overall prevalence of A. cantonensis in B. similariswas 24.6%. A. fulica was the most important intermediary host of A. cantonensis in the studied area andB. similaris was secondary in importance for A. cantonensis transmission dynamics.

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Section: Discussionmentioning

confidence: 99%

Angiostrongylus cantonensis infection in molluscs in the municipality of São Gonçalo, a metropolitan area of Rio de Janeiro, Brazil: role of the invasive species Achatina fulica in parasite transmission dynamics

Oliveira

Gentile

Júnior

et al. 2015

Mem. Inst. Oswaldo Cruz

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“…In Brazil, R. norvegicus and R. rattus have been found naturally infected by A. cantonensis in the state of Pará ( Moreira et al 2013 ), the first species also having been found infected in ES ( Caldeira et al 2007 ), RJ ( Simões et al 2011 ) and more recently in RS ( Cognato et al 2013 ), confirming the parasite’s endemicity. Different species of terrestrial gastropods and freshwater mollusks are involved in the lifecycle of A. cantonensis, acting as intermediate hosts.…”

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confidence: 86%

“…Inter and intraspecific genetic variations in the genus Angiostrongylys have been reported using partial sequencing of the cytochrome oxidase subunit I (COI) gene ( Jefferies et al 2009 ), confirming its use as a molecular marker for differentiation of geographic isolates of A. cantonensis ( Eamsobhana et al 2010 , Simões et al 2011 , Monte et al 2012 , Tokiwa et al 2012 ). Three haplotypes of A. cantonensis (ac5, ac8 and ac9) have been reported in Brazil until now, considering the eight previously related (ac1-ac8) ( Tokiwa et al 2012 ).…”

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confidence: 87%

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Monte

Gentile

Garcia

et al. 2014

Mem. Inst. Oswaldo Cruz

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Angiostrongylus cantonensis is the etiologic agent of eosinophilic meningoencephalitis in humans. Cases have been recorded in many parts of the world, including Brazil. The aim of this study was to compare the differences in the biology and morphology of two different Brazilian haplotypes of A. : ac8 and ac9. A significantly larger number of L1 larvae eliminated in the faeces of rodents at the beginning of the patent period was observed for ac9 haplotype and compared to the total of L1 larvae eliminated, there was a significant difference between the two haplotypes. The ac9 haplotype showed a significant difference in the proportion of female and male specimens (0.6:1), but the same was not observed for ac8 (1.2:1). The morphometric analysis showed that male and female specimens isolated from ac8 haplotype were significantly larger with respect to body length, oesophagus length, spicule length (male) and distance from the anus to the rear end (female) compared to specimens from ac9. The morphological analysis by light microscopy showed little variation in the level of bifurcations at the lateral rays in the right lobe of the copulatory bursa between the two haplotypes. The biological, morphological and morphometric variations observed between the two haplotypes agree with the observed variation at the molecular level using the cytochrome oxidase subunit I marker and reinforce the possible influence of geographical isolation on the development of these haplotypes.

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“…For example, ribosomal DNA sequences were used to assess metastrongylid nematode relationships (Carreno & Nadler, 2003) or to help survey larvae from mollusk intermediate hosts (Fontanilla & Wade, 2008; Qvarnstrom, Sullivan, Bishop, Hollingsworth, & da Silva, 2007; Qvarnstrom et al., 2010), and ~360‐bp region of the cytochrome c oxidase 1 ( CO 1) gene was used to assess relationships to other species of Angiostrongylus (Eamsobhana et al., 2010). To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, CO 1 and cytochrome b ( CYTB ), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants (Aghazadeh et al., 2015; Dalton, Fenton, Cleveland, Elsmo, & Yabsley, 2017; Dusitsittipon, Criscione, Morand, Komalamisra, & Thaenkham, 2017; Dusitsittipon, Thaenkham, Watthanakulpanich, Adisakwattana, & Komalamisra, 2015; Eamsobhana, Song, et al., 2017; Eamsobhana, Yong, et al., 2017; Lv et al., 2012; Monte et al., 2012; Moreira et al., 2013; Nakaya et al., 2013; Okano et al., 2014; Rodpai et al., 2016; Simoes et al., 2011; Tokiwa et al., 2012, 2013; Vitta et al., 2016; Yong, Eamsobhana, Song, Prasartvit, & Lim, 2015; Yong, Song, Eamsobhana, Goh, & Lim, 2015; Yong, Song, Eamsobhana, & Lim, 2016). …”

Section: Introductionmentioning

confidence: 99%

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Dusitsittipon

Criscione

Morand

et al. 2018

Evolutionary Applications

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Angiostrongylus cantonensis, the rat lungworm, is a zoonotic pathogen that is one of the leading causes of eosinophilic meningitis worldwide. This parasite is regarded as an emerging pathogen with a global range expansion out of southeastern Asia post‐WWII. To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, cytochrome c oxidase 1 (CO1) and cytochrome b (CYTB), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants. In order to look for more global evolutionary patterns, we herein provide a collective phylogenetic assessment using the six available whole mtDNA genome samples that have been tagged as A. cantonensis, A. malaysiensis, or A. mackerrasae along with all other GenBank CO1 and CYTB partial sequences that carry these species identifiers. The results reveal three important complications that researchers will need to be aware of, or will need to resolve, prior to conducting future molecular evolutionary studies on A. cantonensis. These three problems are (i) incongruence between taxonomic identifications and mtDNA variants (haplotypes or whole mtDNA genome samples), (ii) the presence of a CYTB mtDNA pseudogene, and (iii) the need to verify A. mackerrasae as a species along with other possible cryptic lineages, of which there is suggestive evidence (i.e., A. cantonensis could be a species complex). We provided a discussion of how these complications are hurdles to our understanding of the global epidemiology of angiostrongyliasis. We call for future studies to be more explicit in morphological traits used for identifications (e.g., provide measurements). Moreover, it will be necessary to repeat prior morphological and life‐history studies while simultaneously using sequence data in order to assess possible associations between critical epidemiological data (e.g., biogeography, virulence/pathology, host species use) and specific lineages.

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Endemic Angiostrongyliasis, Rio de Janeiro, Brazil

Cited by 35 publications

References 11 publications

Angiostrongylus cantonensis infection in molluscs in the municipality of São Gonçalo, a metropolitan area of Rio de Janeiro, Brazil: role of the invasive species Achatina fulica in parasite transmission dynamics

Angiostrongylus cantonensis infection in molluscs in the municipality of São Gonçalo, a metropolitan area of Rio de Janeiro, Brazil: role of the invasive species Achatina fulica in parasite transmission dynamics

Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profiles

Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

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