End effects in field-flow fractionation channels:  theory and means for reducing incremental zone broadening

Giddings, J. Calvin; Schure, Mark R.; Myers, Marcus N.; Velez, George

doi:10.1021/ac00276a028

Cited by 24 publications

(23 citation statements)

References 18 publications

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“…The triangular endpieces of FFF channels lead to crescent-shaped bands which are effectively broader than straight bands. A theoretical examination shows that the extra band broadening can be "diluted" if necessary by increasing L [16]. (End effects can also be reduced by decreasing b as will be described in IG.…”

Section: Ifmentioning

confidence: 97%

Micro‐FFF: Theoretical and practical aspects of reducing the dimensions of field‐flow fractionation channels

Giddings

1993

J Microcolumn Separations

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Abstract. The value of miniaturizing field-flow fractionation (FFF) channels is examined based on theoretical and experimental evidence. While FFF and chromatography are dynamically similar, the dimension analogous to capillary or particle diameter in chromatography is the Brownian excursion distance (t), a "soft" dimension controlled by field strength and thus subject to significant reduction by increasing the field strength. Optimization of the "hard" channel dimensions (length, breadth, thickness) is a more complex matter involving diverse factors including sample dilution and detectability , clogfree operation, edge effects, relaxation time, compatibility with pump and detector systems, and only in some cases the influence of these dimensions on the achievable E.

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Section: Ifmentioning

confidence: 97%

Micro‐FFF: Theoretical and practical aspects of reducing the dimensions of field‐flow fractionation channels

Giddings

1993

J Microcolumn Separations

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“…If is small, the approximation = 24 3 can be used [23,24]. The third term is the sum of instrumental contributions (H i ) such as injection, detection, system dead volume, and flow irregularities [25][26][27]. For a well-constructed FFF apparatus that is being properly operated, the third term will also be small.…”

Section: Separation Efficiencymentioning

confidence: 99%

Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and bioparticles

Yohannes

Jussila

Hartonen

et al. 2011

Journal of Chromatography A

137

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“…Nonionic surfactant molecules adsorb to the particle surface and stabilize the particles by a steric-hindrance mechanism rather than by mutually repulsive negative charge. Therefore, particles stabilized by a nonionic surfactant should be relatively insensitive to electrolyte P6 <50, tR must be calculated by numerical integration of Equation 7.…”

Section: Figure 1 Calculated Total Interaction Energy (Using Equatiomentioning

confidence: 99%

“…The triangular ends that are present in the classical configuration are avoided. These have been shown to be a possible cause of additional band broadening [7,8]. With the geometry used, the axial (elution) flow decreases along the channel, but this is not believed to cause any significant concern, provided that the appropriate theoretical description is used.…”

Section: Introductionmentioning

confidence: 99%

Effects of ionic strength of eluent on retention behavior and on the peak broadening process in hollow fiber flow field‐flow fractionation

Carlshaf

Jönsson

1991

J Microcolumn Separations

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Abstract. The influence of forces on the particles other than the applied cross flow is studied in hollow fiber flow field-flow fractionation (HF'). Such forces can be of either attractive or repelling in nature. It is shown by injecting polystyrene latex beads (0.091 pm in diameter) at different cross flows and by using eluents with different ionic strengths, that there will be disturbances in the concentration profile of the sample irrespective of the type of interacting forces. The retention time and peak broadening were either increased or decreased compared to the theoretical value, depending on the nature of the dominating interaction force. It is, nevertheless, possible to miuimize the total interaction energy between particles and between the particles and the channel wall by choosing a suitable ionic strength.

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End effects in field-flow fractionation channels: theory and means for reducing incremental zone broadening

Cited by 24 publications

References 18 publications

Micro‐FFF: Theoretical and practical aspects of reducing the dimensions of field‐flow fractionation channels

Micro‐FFF: Theoretical and practical aspects of reducing the dimensions of field‐flow fractionation channels

Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and bioparticles

Effects of ionic strength of eluent on retention behavior and on the peak broadening process in hollow fiber flow field‐flow fractionation

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