We report a light-field based method that allows the optical encryption of three-dimensional (3D) volumetric information at the microscopic scale in a single 2D light-field image. The system consists of a microlens array and an array of random phase/amplitude masks. The method utilizes a wave optics model to account for the dominant diffraction effect at this new scale, and the system pointspread function (PSF) serves as the key for encryption and decryption. We successfully developed and demonstrated a deconvolution algorithm to retrieve both spatially multiplexed discrete data and continuous volumetric data from 2D light-field images. Showing that the method is practical for data transmission and storage, we obtained a faithful reconstruction of the 3D volumetric information from a digital copy of the encrypted light-field image. The method represents a new level of optical encryption, paving the way for broad industrial and biomedical applications in processing and securing 3D data at the microscopic scale.The ever-increasing amount of information that individuals and organizations are storing, processing and analyzing drives the demand for information security technologies. Various such techniques, including steganography, cryptography and digital watermarking, have been used to enhance the security and privacy of data 1-3 . Since the inception of double random phase encoding (DRPE) 4 , optical technologies have demonstrated remarkable advantages compared to other encryption methodologies. These advantages include system flexibility, multi-dimensional capabilities, and high encryption density with optical signal processing [5][6][7] . Optical systems exhibit an inherent parallel-processing nature, operating on the incident information without the need to sequentially process data [5][6][7] . The rapid development of optical cryptosystems takes advantage of the many degrees of freedom available with both real and phase-space optical parameters, such as amplitude, polarization, wavelength, and phase [8][9][10][11][12][13] . Several optical encryption techniques employing variations of the classical Fourier transform based DRPE system have been further developed, including Fresnel transform (FST), Fractional Fourier transform (FRT), Hartley Transform (HT), Gyrator Transform (GT), and Linear Canonical Transform (LCT) [14][15][16][17][18][19] . The quantum nature of light has also been explored as a security key in quantum communications 20,21 . Recently, various approaches have been reported for the optical encryption of 3D objects at the macroscopic scale (millimeters, centimeters to meters) [22][23][24][25][26][27][28][29][30][31] . However, the existing methods are ineffective at the microscopic scale, despite the ever-growing demand in this regime. This challenge is mainly caused by two factors. First, in macroscopic scenes, the light-field information can be effectively analyzed using geometrical optics, but because diffraction now plays a crucial role, a wave optics model must be considered in the recordin...