Encrypted antimicrobial and antitumoral peptides recovered from a protein-rich soybean (Glycine max) by-product

Freitas, Cyntia Silva; Vericimo, Mauricio Afonso; Silva, Manuela Leal da; Costa, Giovani Carlo Veríssimo da; Paschoalin, Vânia Margaret Flosi; Pereira, Patrícia Ribeiro

doi:10.1016/j.jff.2019.01.024

Cited by 37 publications

(18 citation statements)

References 51 publications

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“…The peptides in each fraction (F1-F6) were identified by nano-LC-MS/MS according to Freitas et al [56]. Nano-LC-MS/MS analyses were performed in triplicate and peptide chromatography separation was performed using a nano-LC Proxeon EASY-nLCII (Thermo Scientific) coupled to a Quadrupole-Orbitrap Q-Exactive Plus mass spectrometer (Thermo Scientific).…”

Section: Nano-liquid Chromatography and Mass Spectrometry (Nano-lc-ms...mentioning

confidence: 99%

Identification of Antibacterial Peptide Candidates Encrypted in Stress-Related and Metabolic Saccharomyces cerevisiae Proteins

et al. 2022

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The protein-rich nature of Saccharomyces cerevisiae has led this yeast to the spotlight concerning the search for antimicrobial peptides. Herein, a <10 kDa peptide-rich extract displaying antibacterial activity was obtained through the autolysis of yeast biomass under mild thermal treatment with self-proteolysis by endogenous peptidases. Estimated IC50 for the peptide pools obtained by FPLC gel filtration indicated improved antibacterial activities against foodborne bacteria and bacteria of clinical interest. Similarly, the estimated cytotoxicity concentrations against healthy human fibroblasts, alongside selective indices ≥10, indicates the fractions are safe, at least in a mixture format, for human tissues. Nano-LC-MS/MS analysis revealed that the peptides in FPLC fractions could be derived from both induced-proteolysis and proteasome activity in abundant proteins, up-regulated under stress conditions during S. cerevisiae biomass manufacturing, including those coded by TDH1/2/3, HSP12, SSA1/2, ADH1/2, CDC19, PGK1, PPI1, PDC1, and GMP1, as well as by other non-abundant proteins. Fifty-eight AMP candidate sequences were predicted following an in silico analysis using four independent algorithms, indicating their possible contribution to the bacterial inactivation observed in the peptides pool, which deserve special attention for further validation of individual functionality. S. cerevisiae-biomass peptides, an unconventional but abundant source of pharmaceuticals, may be promissory adjuvants to treat infectious diseases that are poorly sensitive to conventional antibiotics.

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Section: Nano-liquid Chromatography and Mass Spectrometry (Nano-lc-ms...mentioning

confidence: 99%

Identification of Antibacterial Peptide Candidates Encrypted in Stress-Related and Metabolic Saccharomyces cerevisiae Proteins

et al. 2022

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“…Well, practically, this cannot be achieved, but it is always good to have a lofty target to pursue. Soybean protein meal (Glycine max) has been found to yield antimicrobial and antitumoral peptides [121], while antioxidative anthraquinones can be extracted from the roots of Morinda citrifolia, a plant belonging to the coffee family [168], and waste peels of the Juçara fruit have been found to be rich in phenolic compounds [29]. Hydrothermal treatment which is known to enhance enzymatic cellulose degradation [344] has been carried out on several other undervalued waste streams like olive prunes and peanut shells, indicating a swathe of potential side streams to be harnessed in a circular bio-economy knitting the agro-food sector with the chemical-pharmafood sector.…”

Section: Discussion-systematic Reviewmentioning

confidence: 99%

Circular Bio-economy—Paradigm for the Future: Systematic Review of Scientific Journal Publications from 2015 to 2021

Govindarajan

2021

Circ.Econ.Sust.

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While ‘renewable’ is the keyword in a bioeconomy and resource conservation is the motivation behind a circular economy, a circular bioeconomy is one in which waste streams from renewable bio-resources are looped back into the technosphere—open-loop or closed-loop recycling or conversion from matter to energy. This systematic review brings together 385 publications from 2015 to 2021, originating from 50 countries and appearing in 150 journals, into a coherent account of the status quo of published research on circular bioeconomy. The numbers bear testimony to the growing interest in this field of research. Germany is the leading contributor to the scientific literature base (10%), while the Journal of Cleaner Production (9%) tops the list of journals in the fray. The methodology adopted has been clearly explained, and the discussion has been segmented into sub-sections and sub-sub-sections to do justice to the diversity of the nature of the publications. A little flexibility in organisation of the flow of the text has been availed of, to improve readability. The circular bioeconomy can be visualised as a set of ‘many through many to many’ relationships, enabling both economies of scale and scope in the longer run. This calls for extensive collaboration and cooperation among the numerous stakeholders involved. Several barriers will have to be overcome. Technology impact assessments and sustainability risk appraisals need to be carried out in order to ensure and convince stakeholders that they are on the right path. But as one knows and will appreciate, challenges lurk where there exist opportunities to be availed of, to replace the take-make-use-dispose paradigm of a linear economy to the grow-make-use-restore alternative. Graphical abstract

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“…The latter, CAMP R3 (Collection of Antimicrobial Peptides), comprises deduced and experimentally proved AMP sequences and also offers prediction tools. Encrypted AMPs were recovered from soybean meal aqueous extract after prediction through analyses using free algorithms provided by CAMP R3 [ 70 ]. In the study, hydrolysis was carried out by thermal activation of proteases already present in the extract, and the peptides were recovered through membrane ultrafiltration and chromatographic fractionation.…”

Section: Production Of Baps and Hydrolysatesmentioning

confidence: 99%

Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens

et al. 2023

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Bioactive peptides (BAPs) derived from food proteins have been extensively studied for their health benefits, majorly exploring their potential use as nutraceuticals and functional food components. These peptides possess a range of beneficial properties, including antihypertensive, antioxidant, immunomodulatory, and antibacterial activities, and are naturally present within dietary protein sequences. To release food-grade antimicrobial peptides (AMPs), enzymatic protein hydrolysis or microbial fermentation, such as with lactic acid bacteria (LAB), can be employed. The activity of AMPs is influenced by various structural characteristics, including the amino acid composition, three-dimensional conformation, liquid charge, putative domains, and resulting hydrophobicity. This review discusses the synthesis of BAPs and AMPs, their potential for controlling foodborne pathogens, their mechanisms of action, and the challenges and prospects faced by the food industry. BAPs can regulate gut microbiota by promoting the growth of beneficial bacteria or by directly inhibiting pathogenic microorganisms. LAB-promoted hydrolysis of dietary proteins occurs naturally in both the matrix and the gastrointestinal tract. However, several obstacles must be overcome before BAPs can replace antimicrobials in food production. These include the high manufacturing costs of current technologies, limited in vivo and matrix data, and the difficulties associated with standardization and commercial-scale production.

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Encrypted antimicrobial and antitumoral peptides recovered from a protein-rich soybean (Glycine max) by-product

Cited by 37 publications

References 51 publications

Identification of Antibacterial Peptide Candidates Encrypted in Stress-Related and Metabolic Saccharomyces cerevisiae Proteins

Identification of Antibacterial Peptide Candidates Encrypted in Stress-Related and Metabolic Saccharomyces cerevisiae Proteins

Circular Bio-economy—Paradigm for the Future: Systematic Review of Scientific Journal Publications from 2015 to 2021

Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens

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