Encoding of plant odours by receptor neurons in the pine weevil (Hylobius abietis) studied by linked gas chromatography-electrophysiology

Wibe, Atle; Mustaparta, Hanna

doi:10.1007/bf00194987

Cited by 37 publications

(18 citation statements)

References 32 publications

(40 reference statements)

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“…Decisions regarding whether to progress towards an odor source, or to abort, are probably based upon a balance mechanism in which attractive and anti-attractive/repellent inputs in the combined 'odor bouquet' are weighed against each other. In the insect olfactory system, specific olfactory receptor neurons (ORNs) constitute separate input channels, each detecting compounds from different chemical and biological categories (Andersson et al, 2009;Bengtsson et al, 2009;Larsson et al, 2001;Mustaparta, 1975;Wibe and Mustaparta, 1996). ORN axons project to the glomeruli of the primary olfactory center, the antennal lobe, where integration of odor input takes place through lateral interactions between glomeruli (Olsen and Wilson, 2008;Shang et al, 2007;Silbering et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Andersson

Larsson

Blaženec

et al. 2010

Journal of Experimental Biology

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SUMMARYWe identified several compounds, by gas chromatographic-electroantennographic detection (GC-EAD), that were antennally active in the bark beetle Ips typographus and also abundant in beetle-attacked spruce trees. One of them, 1,8-cineole (Ci), strongly inhibited the attraction to pheromone in the field. Single-sensillum recordings (SSRs) previously showed olfactory receptor neurons (ORNs) on I. typographus antennae selectively responding to Ci. All Ci neurons were found within sensilla co-inhabited by a pheromone neuron responding to cis-verbenol (cV); however, in other sensilla, the cV neuron was paired with a neuron not responding to any test odorant. We hypothesized that the colocalization of ORNs had a functional and ecological relevance. We show by SSR that Ci inhibited spontaneous activity of the cV neuron only in sensilla in which the Ci neuron was also present. Using mixtures of cV and Ci, we further show that responses to low doses (1-10ng) of cV were significantly reduced when the colocalized Ci neuron simultaneously responded to high doses (1-10mg) of Ci. This indicated that the response of the Ci neuron, rather than ligand-receptor interactions in the cV neuron, caused the inhibition. Moreover, cV neurons paired with Ci neurons were more sensitive to cV alone than the ones paired with the non-responding ORN. Our observations question the traditional view that ORNs within a sensillum function as independent units. The colocalization of ORNs might sharpen adaptive responses to blends of semiochemicals with different ecological significance in the olfactory landscape.

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Section: Introductionmentioning

confidence: 99%

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Andersson

Larsson

Blaženec

et al. 2010

Journal of Experimental Biology

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“…Also the pink bollworm moth, Pectinophora gossypiella, one of the major pests on the β-caryophyllene rich cotton plants, has specific ORNs with high sensitivty to β-caryophyllene (Tömmerdal, 1995). In other studies, the use of gas chromatography linked to electrophysiology has been attempted to test single ORNs in oligophagous and polyphagous species for, in principle, all of the naturally produced constituents in the relevant plants (Blight et al, 1995;Wibe & Mustaparta, 1996;Wibe et al, 1997;Rostelien et al, 2000a,b;Stranden et al, 2002) Stensmyr et al, 2001Barata et al, 2002). Interestingly, in two beetle species (Hylobius abietis and Phoracanta semipunctata) considered as oligophagous, many different ORN types have so far been identified that respond to compounds common in many plants.…”

Section: Orns In Oligophagous Versus Polyphagous Speciesmentioning

confidence: 99%

Encoding of plant odour information in insects: peripheral and central mechanisms

Mustaparta

2002

Entomologia Exp Applicata

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Insects are suitable model organisms for studying mechanisms underlying olfactory coding and olfactory learning, by their unique adaptation to host plants in which the chemical senses are essential. Recent molecular biological studies have shown that a large number of genes in insects and other organisms are coding for olfactory receptor proteins. In general, one receptor type seems to be expressed in each neurone. The functional characterisations of olfactory receptor neurones have been extensive in certain insect species, demonstrating a fine-tuning of single neurones to biologically relevant odourants; both insect and plant produced volatiles. Stained neurones of the same functional type have been shown to project in one and the same glomerular unit in the primary olfactory centre, the antennal lobe. This corresponds to molecular biological studies, showing projections in one glomerulus by neurones expressing the same receptor type. Comparison of these findings with physiological and morphological characterisations of antennal lobe neurones has indicated correspondence between input and output of the glomerular units. Examples are presented from studies of heliothine moths. From the antennal lobe, the olfactory information is further conveyed to the mushroom bodies, particularly important for learning, and the lateral protocerebrum, a premotoric area. The three brain areas are regions of synaptic plasticity important in learning of odours, which is well studied in the honeybee but also in species of moths.

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“…During recent years the use of GC-SCR has provided knowledge about the molecular receptive ranges of plant odour RNs in a few species of weevils, other beetles and heliothine moths (Blight et al 1995;Wibe and Mustaparta 1996;Wibe et al 1997;Røstelien et al 2000aRøstelien et al , b, 2005Stensmyr et al 2001;Barata et al 2002;Stranden et al 2002;2003a, b;Bichão et al 2003Bichão et al , 2005. The present results on the oligophagous weevil Anthonomus rubi, whose larvae and adults use strawberry as host for feeding and reproduction, are interesting both to understand olfaction in this species, and to make comparisons with other species.…”

Section: Discussionmentioning

confidence: 99%

“…Wibe and Mustaparta 1996;Wibe et al 1997;Røstelien et al 2000a, b;Barata et al 2002;Bichão et al 2003Bichão et al , 2005Stranden et al 2002Stranden et al , 2003a. First, the recordings of each neurone were highly reproducible i.e.…”

Section: Criteria For Classification Of Functional Rn Typesmentioning

confidence: 97%

Molecular receptive ranges of olfactory receptor neurones responding selectively to terpenoids, aliphatic green leaf volatiles and aromatic compounds, in the strawberry blossom weevil Anthonomus rubi

et al. 2005

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An important question in insect-plant interactions is which of the numerous plant compounds contribute to the perception of odour qualities in herbivorous insects and are likely to be used as cues in host-searching behaviour. In order to identify which plant-produced volatiles the strawberry blossom weevil Anthonomus rubi detects, we have used electrophysiological recordings from single olfactory neurones linked to gas chromatography and mass spectrometry. We here present 15 receptor neurone types specialised for naturally produced compounds present in the host and nonhost plants and two types for two aggregation pheromone components. The active compounds were terpenoids, aromatic and aliphatic esters, alcohols and aldehydes, some of which are induced by feeding activity of the weevils. The neurones were characterised by a strong response to one or two primary odorants and weaker responses to a few others having similar chemical structure. With one exception, the molecular receptive range of each neurone type was within one chemical group. Enantiomers of linalool separated on a chiral column activated two neurone types with different enantioselectivity. Inhibition by linalool of another neurone type, excited by α-pinene, indicated an additional mechanism for coding the information about this compound. Altogether, detection of 54 compounds by olfactory receptor neurones is shown, of which 40 have been chemically identified in this study. Thus A. rubi has the ability to detect a large number of odorants that may be used in host selection behaviour.

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Encoding of plant odours by receptor neurons in the pine weevil (Hylobius abietis) studied by linked gas chromatography-electrophysiology

Cited by 37 publications

References 32 publications

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Encoding of plant odour information in insects: peripheral and central mechanisms

Molecular receptive ranges of olfactory receptor neurones responding selectively to terpenoids, aliphatic green leaf volatiles and aromatic compounds, in the strawberry blossom weevil Anthonomus rubi

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