Encapsulation of organophosphorus acid anhydrolase (OPAA) in nanostructured materials for the detection and decontamination of chemical warfare agents

Encapsulation of a Nerve Agent Detoxifying Enzyme by a Mesoporous Zirconium Metal–Organic Framework Engenders Thermal and Long-Term Stability

“…To the our knowledge, the OPAA loading capacity of PCN-128y is much higher than that of any other porous material yet examined. 15,33 The N 2 adsorption isotherm of OPAA@PCN-b128y a lower N 2 uptake capacity than that of PCN-128y (Figure 2a). The density functional theory (DFT) pore size distribution analysis of PCN-128y and OPAA@PCN-128y shows that the pore volume corresponding to the triangular channels (1.2−1.5 nm) of PCN-128y drops from 0.19 to 0.14 cm 3 /g, while the incremental pore volume corresponding to the hexagonal channels (3.3−4.0 nm) drops from 0.52 to 0.13 cm 3 /g after OPAA encapsulation (Figure 2b).…”

“…Nevertheless, due to a lack of long-range ordering, the loading capacity of OPAA in FMS is usually very low (<0.01 wt %). 15 In addition, enhanced thermal and long-term stability of immobilized OPAA has not yet been achieved. Therefore, the development of new host materials is important for targeting both high enzyme loading and enhanced stability for OPAA immobilization.…”

“…Significant effort has been devoted to developing useful materials as solid supports for encapsulation of OPAA to afford stable and convenient formulation for use in chemical agent detoxification and detection. Previously, various materials including polymers, silica gel, functionalized mesoporous silicas (FMS), , and porous silica (PSi) have been used to immobilize OPAA. For example, FMS encapsulated OPAA shows enhanced tolerance to organic solvents.…”

“…For example, FMS encapsulated OPAA shows enhanced tolerance to organic solvents. Nevertheless, due to a lack of long-range ordering, the loading capacity of OPAA in FMS is usually very low (<0.01 wt %) . In addition, enhanced thermal and long-term stability of immobilized OPAA has not yet been achieved.…”