Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

Arias-P閞ez, Eliana; Lecona-Guzm醤, Carlos Alberto; Guti閞rez-Miceli, Federico Antonio; Montes-Molina, Joaqu韓 Adolfo; Ruíz-Lau, Nancy

doi:10.32604/phyton.2021.016004

Cited by 1 publication

(3 citation statements)

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“…The percentage of contaminated explants was evaluated in response to three field treatments. For this study, the methodology described by Arias-Pérez et al (2021) and Ortiz-Gómez et al (2017) was adapted; each treatment corresponded to one accession. Spraying was established biweekly 60 days prior to collection, according to the BBCH scale stages for C. arabica as reported by Arcila-Pulgarín et al (2002).…”

Section: Agronomic Management Of Phytopathogens In the Fieldmentioning

confidence: 99%

“…Treatment two, which involved the intercalated spraying of PC and OC three days prior to collection, reduced in vitro contamination, 45 % fungal and 25 % bacterial, when compared to the unmanaged treatment. Arias-Pérez et al (2021) reported that a three-day pre-harvest management with Captan ® and Agrimycin ® on field plants does not fully control the presence of fungi in vitro (40 % effectiveness). Similarly, López-Gómez et al (2011) reported that spraying 1 g L -1 of Amistar ® (Syngenta, Azoxystrobin 50 %) for three days on Coffea sp.…”

Section: Aseptic Establishment Of In Vitro Explantsmentioning

confidence: 99%

“…There are protocols for the propagation of coffee plants through somatic embryogenesis using young leaves collected from plants established in greenhouses; however, reports of agronomic management in the field prior to in vitro cultivation are scarce. Arias-Pérez et al (2021) describe the application of chemical fungicides three days before the collection of explants in various coffee plantations, with contamination levels ranging from 40 to 100 %. This justifies the need to evaluate multiple disinfection protocols based on the species, phenological stage, and geographical location of mother plant (Silva et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

Dávila

Ruiz-Sesma

Lecona-Guzmán

et al. 2023

agro

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In Chiapas, Mexico, there are high-yielding coffee plants adapted to the local climatic conditions. The presence of microorganisms in the explants makes developing protocols for cloning these genotypes in vitro difficult. The objective of this work was to evaluate how field and in vitro microorganism management affected asepsis and the induction of callogenesis in leaf explants of agronomically important coffee plants. Drought-tolerant genotypes were obtained from aseptic explants and cell cultures using a participatory study, agronomic, and in vitro techniques. Five accessions (ITTGj 1-5) were selected from a 21-year-old Borbon cultivar. The present study was conducted over two flowering cycles, evaluating the effect of field management on explant asepsis on the first and the callogenic response caused by the culture medium, the transport solution, and the genotype, on the second. Sixty days before explant collection, biweekly applications of calcium polysulfide (10 % v v-1), copper oxychloride (2 g L-1), and Zingiber officinale extract (20 g L-1) reduced fungal contamination by 100 % and bacterial contamination by 90 %. Field explant transportation in 200 mg L-1 citric acid and ascorbic acid solution reduced oxidation in explants and in vitro cultures. Proembryogenic friable corns were formed with the combination of 4.4 µM BAP (6-benzylaminopurine) and 7.25 µM 2,4-D (2,4-dichlorophenoxyacetic acid) at 45 days of culture, with genotype four showing the best response. Field management was effective for the aseptic establishment of Coffea arabica cultures in vitro, and the combination of BAP and 2,4-D used was an appropriate growth regulator for the induction of callogenesis.

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Section: Agronomic Management Of Phytopathogens In the Fieldmentioning

confidence: 99%

Section: Aseptic Establishment Of In Vitro Explantsmentioning

confidence: 99%