Encapsulation of hydrophobic drugs in polymeric micelles through co-solvent evaporation: The effect of solvent composition on micellar properties and drug loading

Development and Validation of a Method for Determination of Encapsulation Efficiency of CPT-11/DSPE-mPEG2000 Nanoparticles

“…The encapsulation of drug molecules within the "Trojan horse" nanosystems is the key in harnessing the power of nanotechnology for development of better anti-cancer therapeutics [2]. Because of this, the determination of the encapsulation efficiency (EE) which is defined as the percentage of drug molecules successfully entrapped within the NPs is regarded as a key step in characterizing the quality of the nanoformulations [3,4].…”

“…The DSPE-mPEG2000 is an amphiphilic molecule that is regarded as a GRAS (generally regarded as safe) material by FDA and has been widely used in pharmaceutical research [9]. In this paper, we examined three strategies including dialysis method, centrifugation method and a gel chromatography method for separation of untrapped drug molecule from the micellar system [3,10,11]. The amount of the drug was characterized using high performance liquid chromatography (HPLC) coupled with UVVis detector or UV spectrometry.…”

Investigation into Drug Solubilisation Potential of Sulfonated Calix[4]Resorcinarenes

“…Over the past few decades, the development of solubilisers for hydrophobic drugs as drug delivery systems has attracted much attention among researchers in both pharmaceutical and biomedical fields [1,2]. More than 40% of therapeutic agents identified in combinatorial libraries are classified as hydrophobic [3]; in the UK drugs which possess aqueous solubility below 0.1 mgmL -1 at 25°C are classed as practically insoluble according to the British Pharmacopoeia [4].…”

Investigation on design of stable etoposide-loaded PEG-PCL micelles: effect of molecular weight of PEG-PCL diblock copolymer on thein vitroandin vivoperformance of micelles

“…These micelles have demonstrated their ability to efficiently solubilize lipophilic agents with altered drug pharmacokinetics and in several cases have been reached to clinical trials (Allen et al, 2000;Matsumura et al, 2004;Zhang et al, 2004;Forrest et al, 2008;Lee et al, 2008;Xiong et al, 2008) Micelles made up of core-shell structure are normally fabricated by a self-assembly approach using amphiphilic block copolymers in a selective solvent in which the hydrophobic core part provides a cargo space for solubilization of various lipophilic drugs (Aliabadi et al, 2007;Molavi et al, 2008). The core region surrounded by hydrophilic corona part, that is, of higher molecular weight poly (ethylene glycol) (PEG) provides stabilization of micelles with no need of additional stabilizers.…”