Encapsulation of human endometrial stem cells in chitosan hydrogel containing titanium oxide nanoparticles for dental pulp repair and tissue regeneration in male Wistar rats

Hoveizi, Elham; Naddaf, Hadi; Ahmadianfar, Sina; Gutmann, James L.

doi:10.1016/j.jbiosc.2022.12.009

Cited by 9 publications

(8 citation statements)

References 43 publications

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“…Previous study used chitosan as a scaffolding to carry human endometrial stem cells (EnSCs) in VPT. [ 45 ] Chitosan is bio-compatible, bio-degradable, and non-toxic, which is capable to use in medical applications. Gelatin sponge was also reported to be used in rat direct pulp capping models.…”

Section: Discussionmentioning

confidence: 99%

METTL3 enhances dentinogenesis differentiation of dental pulp stem cells via increasing GDF6 and STC1 mRNA stability

et al. 2023

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Background The dentinogenesis differentiation of dental pulp stem cells (DPSCs) is controlled by the spatio-temporal expression of differentiation related genes. RNA N6-methyladenosine (m6A) methylation, one of the most abundant internal epigenetic modification in mRNA, influences various events in RNA processing, stem cell pluripotency and differentiation. Methyltransferase like 3 (METTL3), one of the essential regulators, involves in the process of dentin formation and root development, while mechanism of METTL3-mediated RNA m6A methylation in DPSC dentinogenesis differentiation is still unclear. Methods Immunofluorescence staining and MeRIP-seq were performed to establish m6A modification profile in dentinogenesis differentiation. Lentivirus were used to knockdown or overexpression of METTL3. The dentinogenesis differentiation was analyzed by alkaline phosphatase, alizarin red staining and real time RT-PCR. RNA stability assay was determined by actinomycin D. A direct pulp capping model was established with rat molars to reveal the role of METTL3 in tertiary dentin formation. Results Dynamic characteristics of RNA m6A methylation in dentinogenesis differentiation were demonstrated by MeRIP-seq. Methyltransferases (METTL3 and METTL14) and demethylases (FTO and ALKBH5) were gradually up-regulated during dentinogenesis process. Methyltransferase METTL3 was selected for further study. Knockdown of METTL3 impaired the DPSCs dentinogenesis differentiation, and overexpression of METTL3 promoted the differentiation. METTL3-mediated m6A regulated the mRNA stabiliy of GDF6 and STC1. Furthermore, overexpression of METTL3 promoted tertiary dentin formation in direct pulp capping model. Conclusion The modification of m6A showed dynamic characteristics during DPSCs dentinogenesis differentiation. METTL3-mediated m6A regulated in dentinogenesis differentiation through affecting the mRNA stability of GDF6 and STC1. METTL3 overexpression promoted tertiary dentin formation in vitro, suggesting its promising application in vital pulp therapy (VPT).

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Section: Discussionmentioning

confidence: 99%

METTL3 enhances dentinogenesis differentiation of dental pulp stem cells via increasing GDF6 and STC1 mRNA stability

et al. 2023

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“…177 Hoveizi et al encapsulated human endometrial stem cells in chitosan hydrogel containing titanium oxide nanoparticles for dental pulp repair. 178 Results show the combination of stem cells with chitosan/TiO 2 exhibited a synergistic effect on each other and increased the speed and quality of dentine repair. Lopes et al also fabricated a nanohydroxyapatite/chitosan biodegradable scaffold sterilized by supercritical CO 2 that enabled the production of a costeffective and environment-friendly scaffolds.…”

Section: Chitosan-based Biomaterials For Dentistrymentioning

confidence: 94%

“…A regenerative dental procedure including dentine-pulp complex regeneration is an alternative approach for treating severe pulp exposure to pulp capping and various pulpotomy procedures . Hoveizi et al encapsulated human endometrial stem cells in chitosan hydrogel containing titanium oxide nanoparticles for dental pulp repair . Results show the combination of stem cells with chitosan/TiO 2 exhibited a synergistic effect on each other and increased the speed and quality of dentine repair.…”

Section: Chitosan-based Biomaterials For Dentistrymentioning

confidence: 99%

Chitosan-Based Smart Biomaterials for Biomedical Applications: Progress and Perspectives

Budiarso,

Rini,

Tsalsabila

et al. 2023

ACS Biomater. Sci. Eng.

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Over the past decade, smart and functional biomaterials have escalated as one of the most rapidly emerging fields in the life sciences because the performance of biomaterials could be improved by careful consideration of their interaction and response with the living systems. Thus, chitosan could play a crucial role in this frontier field because it possesses many beneficial properties, especially in the biomedical field such as excellent biodegradability, hemostatic properties, antibacterial activity, antioxidant properties, biocompatibility, and low toxicity. Furthermore, chitosan is a smart and versatile biopolymer due to its polycationic nature with reactive functional groups that allow the polymer to form many interesting structures or to be modified in various ways to suit the targeted applications. In this review, we provide an up-to-date development of the versatile structures of chitosan-based smart biomaterials such as nanoparticles, hydrogels, nanofibers, and films, as well as their application in the biomedical field. This review also highlights several strategies to enhance biomaterial performance for fast growing fields in biomedical applications such as drug delivery systems, bone scaffolds, wound healing, and dentistry.

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“…Rouget discovered the compound in 1859 by utilizing a hot potassium hydroxide solution. Later, Gilson identified the occurrence of glucosamine in it, and concurrently, a similar term, “Chitosan”, was used by Hopper-Seyler [ 20 , 21 , 22 , 23 ]. Structural analysis of chitin and chitosan was conducted in the 1930s by diverse techniques, including X-ray diffraction, enzyme-related methods and infrared spectroscopy.…”

Section: Historical Perspective Of Chitosanmentioning

confidence: 99%

“…The chitosan produced from the mushrooms exhibits a small molecular mass disparity and degree of deacetylation in comparison to the product obtained from seafood. Deproteinization, demineralization, and decolorization are the three key stages that make up the extraction of chitin [ 20 ]. Acids are used to remove inorganic components in the conventional chemical process, strong inorganic alkalis are used to extract proteins (often at 50–60 °C), and oxidizers are used to remove color.…”

Section: Production Of Hydrogel Chitinmentioning

confidence: 99%

Role of Chitosan Hydrogels in Clinical Dentistry

Arora,

Das,

Alqarni

et al. 2023

Gels

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Biopolymers are organic polymers that can be treated into intricate designs with porous characteristics that mimic essential biologic components. Due to their superior biosafety, biodegradability, biocompatibility, etc., they have been utilized immensely in biomedical engineering, regeneration, and drug delivery. To obtain the greatest number of results, a literature search was undertaken in scientific search engines utilizing keywords. Chitosan is used in a variety of medical sectors, with the goal of emphasizing its applications and benefits in the clinical dental industry. Chitosan can be dissolved in liquid form and combined with other substances to create a variety of products, including fibers, hydrogels, membranes, microspheres, resins, sponges, pastes, tablets, and micro granules. Chitosan has been studied in a variety of dental applications. Chitosan is used in the prevention of caries and wear, in pulpotomy to accelerate osteogenesis in guided tissue regeneration due to its hemostatic property, and primarily to benefit from its antimicrobial activity by adding it to materials, such as glass ionomer cement, calcium hydroxide, and adhesive systems. With its antibacterial activity and biocompatibility, chitosan is leading the pack as a promising ingredient in the production of dental materials. The current review provides an update on the background, fundamentals, and wide range of uses of chitosan and its gels in dental science.

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Encapsulation of human endometrial stem cells in chitosan hydrogel containing titanium oxide nanoparticles for dental pulp repair and tissue regeneration in male Wistar rats

Cited by 9 publications

References 43 publications

METTL3 enhances dentinogenesis differentiation of dental pulp stem cells via increasing GDF6 and STC1 mRNA stability

METTL3 enhances dentinogenesis differentiation of dental pulp stem cells via increasing GDF6 and STC1 mRNA stability

Chitosan-Based Smart Biomaterials for Biomedical Applications: Progress and Perspectives

Role of Chitosan Hydrogels in Clinical Dentistry

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