Encapsulation of Berberine in Nano-Sized PLGA Synthesized by Emulsification Method

Abstract

Nanoparticles of PLGA (polylactide glycolic acid) were prepared using biodegradable poly (D, L-lactide-co-glycolide)-75 : 25, by emulsification method using PVA (Mol. Wt. 9000) or didodecyl dimethyl ammonium bromide (DMAB) as surfactant. Nanoparticles were morphologically characterized using scanning electron microscope (SEM) and particle size analyzer. The distribution of size of PLGA nanoparticles was in the range of 48-211 nm. Berberine, a yellow isoquinoline alkaloid that is used as traditional anticancer drug, was loaded on to PLGA nanoparticles by single emulsion as well as multiple emulsion solvent evaporation techniques. Particle size analysis showed an increase in berberine loaded PLGA NP size to 180-310 nm when PVA was used as a stabilizer. Whereas use of DMAB as a stabilizer led to precipitation. In vitro drug release analysis revealed that acidic pH of 5.5 was more suitable for release of berberine than pH 7.4.

Dual-functionalised shellac nanocarriers give a super-boost of the antimicrobial action of berberine

“…The infrared spectrum of free BRB (red line) is characterised by a principal peak at 1602 cm À1 relating to the -C]Nquaternary iminium ion, and a peak at 1504 cm À1 (C]C stretching vibration of aromatic ring). 54 The intensity of these peaks declined when BRB was loaded into shellac nanoparticles and most BRB peaks overlap with the shellac NPs bands, this proves the reaction between BRB and shellac, the broad band for O-H stretching seen for shellac still exists although it has shied slightly as can be seen in Fig. 3A (green line).…”

Nanopharmaceutics: Phytochemical-Based Controlled or Sustained Drug-Delivery Systems for Cancer Treatment

“…The authors theorized that the faster release in acidic medium was due to berberine being an alkaloid and therefore, basic. 102 Lin et al 103 generated liposomal berberine using different lipid compositions. The liposomes produced by a thinfilm hydration/extrusion method and containing 5 mol% PEG exhibited the highest encapsulation efficiency (14%) and a size of 121.6 nm.…”

“…Some adverse reactions such as anaphylactic shock and drug rash have also been associated with intramuscular and intravenous administration. 45 Berberine-loaded PLGA nanoparticles were first prepared by Khemani et al 102 by emulsification method using PVA as surfactant. The resultant spherical NPs were in the range of 180-310 nm with encapsulation efficiencies around 35%.…”

Improvement of the Biological Activity of a New Cobalt(III) Complex through Loading into a Nanocarrier, and the Characterization Thereof

“…To avoid nanoparticle aggregation, a surface‐active polymer PVA (polyvinyl alcohol) was consumed. This stabilizer is mostly used for the preparation of the PLGA nanoparticle because it attaches to PLGA via hydrogen bonding, resulting in the decrement of nanoparticle size. The loading efficiency and in‐vitro release of complex 1 from NPs were also investigated.…”