Encapsulation of adenovirus serotype 5 in anionic lecithin liposomes using a bead-based immunoprecipitation technique enhances transfection efficiency

Abstract

Oncolytic viruses (OVs) constitute a promising class of cancer therapeutics which exploit validated genetic pathways known to be deregulated in many cancers. To overcome an immune response and to enhance its potential use to treat primary and metastatic tumors, a method for liposomal encapsulation of adenovirus has been developed. The encapsulation of adenovirus in non-toxic anionic lecithin-cholesterol-PEG liposomes ranging from 140–180nm in diameter have been prepared by self-assembly around the viral capsid. The encapsulated viruses retain their ability to infect cancer cells. Furthermore, an immunoprecipitation (IP) technique has shown to be a fast and effective method to extract non-encapsulated viruses and homogenize the liposomes remaining in solution. 78% of adenovirus plaque forming units were encapsulated and retained infectivity after IP processing. Additionally, encapsulated viruses have shown enhanced transfection efficiency up to 4× higher compared to non-encapsulated Ads. Extracting non-encapsulated viruses from solution may prevent an adverse in vivo immune response and may enhance treatment for multiple administrations.

Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology

“…VLPs surface modification can be used for several purposes; polymers as polyethylenimine act as adjuvants [60] while polyethylene glycol (PEG) can increase VLPs stability [61][62]. In addition VLPs can be covered with immunostimulatory agents.…”

A Comparative Approach for the Preparation and Physicochemical Characterization of Lecithin Liposomes Using Chloroform and Non‐Halogenated Solvents

“…The present study compares liposomal preparations using solvents, namely ethanol and acetonitrile in comparison with chloroform. These solvents were used to prepare thin films of Lecithin (L-a-phosphatidylcholin) [21]. Lecithin is a negatively charged mixture of phospholipids [1].…”

Liposome-encapsulated plasmid DNA of telomerase-specific oncolytic adenovirus with stealth effect on the immune system

“…Liposome, which is composed of physiological lipids well tolerated by the human body, is one of the most popular nanoparticles used as a drug carrier and has a stealth effect in the in vivo environment by reducing the immunogenicity of the drug-lipid complexes 12 , 23 . There are some previous reports in which encapsulation of an oncolytic virus itself by lipid was tested and showed some potential for use in systemic delivery 24 , 25 . However, this strategy seems to remain problematic in terms of an effective drug delivery system.…”